Lactobacillus casei TH14 and additives could modulate the quality, gas kinetics and the in vitro digestibility of ensilaged rice straw

2020 ◽  
Vol 104 (6) ◽  
pp. 1690-1703
Author(s):  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Waroon Khota
2020 ◽  
Vol 44 (3) ◽  
Author(s):  
Cuk Tri Noviandi ◽  
Dibya Ratnopama ◽  
Ali Agus ◽  
Ristianto Utomo

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.


Author(s):  
Ali Ameen Saeed ◽  
Saja Intisar Abid

This study was conducted in Nutrition Lab. to investigate the effect of the type and level of substitution of urea with ruminant manure, M (sheep, cow and buffalo) on basis of nitrogen (N) content on the nutritive value of rice straw silage (RSS). Accordingly silages were nominated as, S-RSS, C-RSS and B-RSS. Urea (U) was substituted with dried manure at 6 combinations, 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50 of U:M. Silage samples were prepared by treating chopped straw with pre-treated solution contained 10% low quality debis and 2% urea. Results showed that lower (P˂0.01) DM loss (11.4%) was observed in S-RSS, and with addition of urea only (3.6%).        Samples of S-RSS and C-RSS recorded higher (P˂0.01) Fleig points (Fp) as compared with those prepared by the addition of B-RSS, 60.42, 55.58 and 49.59 respectively. Reduction (P˂0.01) in this parameter was noticed in samples prepared with a combination of 100:0. Aerobic stability (AS) was a reduced (P˂0.01) in samples prepared by addition of S-RSS by 15 and 13 hours in comparison with samples of C-RSS and B-RSS respectively. Samples prepared with combination of 100:0 were prior (P˂0.01) as compared with other samples.        Results also showed an increase (P˂0.01) in in vitro digestibility of organic matter (IVOMD) in samples of S-RSS in comparison with samples prepared by addition of C-RSS and B-RSS, 49.99, 44.59 and 42.77% respectively. Samples prepared with combination of 100:0 recorded lower (P˂0.05) in vitro digestibility of dry matter (IVDMD) as compared with combinations of 70:30 and 60:40 of U: M, 40.52, 45.36 and 45.94% respectively.


2020 ◽  
Vol 6 (3) ◽  
pp. 109 ◽  
Author(s):  
Chanon Suntara ◽  
Anusorn Cherdthong ◽  
Suthipong Uriyapongson ◽  
Metha Wanapat ◽  
Pin Chanjula

The objective of this study was to compare the effects of Crabtree-negative ruminal yeast and Crabtree-positive yeast in ensiled rice straw (RS) on the ensilage quality, nutritive value, and microorganism composition, including the evaluation of the ensiled RS using the in vitro gas production technique. The experiment was conducted in a 4 × 3 factorial arrangement in a randomized complete design. Factor A was yeast species with no inoculant, Crabtree-negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20), and Crabtree-positive yeast (Saccharomyces cerevisae), whereas factor B was ensilage times (7, 14, and 21 days). The rate of growth was revealed to be lower in Crabtree-positive yeasts than the other Crabtree-negative yeast strains (p < 0.01). RS ensiled with S. cerevisiae showed decreased dry matter (DM) content by 9.0% when compared to the sample without a yeast inoculant. In addition, organic matter (OM) content was greater (p < 0.01) for P. kudriavzevii KKU20 than C. tropicalis KKU20 and without an inoculant. Neutral detergent fiber (NDF) content was significantly decreased (p < 0.01) by yeast inoculants by about 2.75% when compared to the control group. Lactic acid bacteria (LAB) and aerobic bacteria were low (p < 0.05) when yeasts were added. However, no interaction was found between yeast and ensilage times on the quality of ensiled RS (p > 0.05). The P. kudriavzevii KKU20 addition was associated with the highest value (p < 0.01) of gas produced—an insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h—when compared with S. cerevisiae or the control group. The highest in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed in RS ensiled with P. kudriavzevii KKU20 for 14 days (p < 0.01, p < 0.05). The maximum total volatile fatty acids (VFAs) at 4 and 8 h of incubation and the mean value were observed in RS ensiled with P. kudriavzevii KKU20 (p < 0.01). RS ensiled with all yeast strains showed an increased propionate concentration at 8 h (p < 0.01). In conclusion, ensiling RS with isolated Crabtree-negative ruminal yeasts could benefit feed digestion and in vitro gas production more than Crabtree-positive yeast does. P. kudriavzevii KKU20, an isolated Crabtree-negative ruminal yeast used to treat RS, had the highest potential for increasing cumulative gas production and enhancing in vitro digestibility.


Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 674
Author(s):  
Asavela Ngalavu ◽  
Hailong Jiang ◽  
Saeed El-Ashram ◽  
Guillermo Tellez-Isaias ◽  
Mohammed Hamdy Farouk ◽  
...  

Feed fiber composition is usually considered as one of the factors that have an impact on digestive tract microbiota composition. The investigations on the level of fermentation and in-vitro digestibility of different fibers are not well understood. The aim of the current study is to determine the effect of different fiber sources on intestinal nutrient digestibility, hindgut fermentation, and microbial community composition under in vitro conditions using pigs’ hindgut as a model. The experimental treatment diets contained alfalfa hay, cornstalk, and rice straw. Cornstalk treatment displayed higher digestibility compared to alfalfa hay and rice straw; similar results were observed with in-vitro digestibility using intestinal digesta. Firmicutes were the most abundant phyla (Firmicutes = 89.2%), and Lactobacillus were the prominent genera (75.2%) in response to alfalfa compared to rice straw and cornstalk treatments. In simulated in-vitro digestion, corn stalk fiber improved dry matter digestibility; rice straw fiber improved volatile fatty acid content and fermentation efficiency. Alfalfa fiber improved the thickness of deposited Firmicutes and Lactobacillus.


1992 ◽  
Vol 119 (1) ◽  
pp. 27-33 ◽  
Author(s):  
J. Vadiveloo

SummaryFour varieties of rice straw, MRl, MR71, MR84 and MR27, were separated into the following botanical fractions; inflorescence, stem, leaf blade and leaf sheath. The relative proportion of each fraction in whole straw was estimated and analysed for its chemical composition and in vitro digestibility (IVD). The composition and IVD of whole straw was then calculated. Cluster analysis showed that MRl was distinct from the other varieties. This could be attributed to a high proportion of stem (32·3%), high IVD (36·9%), low insoluble ash (4·0%) and a low silica content (2·7%) in the whole straw. The genetic distance between any two varieties ranged from 5·4 × 10-3 to 47·8 × 10-3 and was largest between MRl and the other varieties. Cluster analysis also showed that within a variety, the chemical composition and IVD of the stem, leaf blade and leaf sheath were similar, suggesting that sampling errors may be reduced if the inflorescence, which comprises 7–13% of the whole straw, is excluded when the material is analysed. Stepwise and canonical discriminant analyses indicated that the important variables to be measured when comparing straw varieties were IVD, crude protein and insoluble ash.


2021 ◽  
Vol 788 (1) ◽  
pp. 012052
Author(s):  
D Muthia ◽  
E B Laconi ◽  
M Ridla ◽  
A Jayanegara ◽  
R Ridwan ◽  
...  

2020 ◽  
Vol 01 (02) ◽  
pp. 36-44
Author(s):  
R. Sultana ◽  
D. K. Jamee ◽  
S. Islam ◽  
S. M. A. Islam ◽  
M. R. Amin ◽  
...  

An experiment of treated and ensiled wet rice straw with urea and molasses was performed to explore the chemical composition, physical quality, in vitro digestibility and its potentiality as a quality feed for ruminants. In this experiment, plastic containers were used to preserve chopped wet rice straw under airtight condition based on the treatment as T1 (wet rice straw only), T2 (straw with 5% molasses), T3 (straw with 5% urea), T4 (straw with 5% urea and 5% molasses) and T5 (straw with 10% urea and 5% molasses) to analyze chemical composition, physical quality, metabolizable energy (ME) content, in vitro organic matter digestibility (OMD) and in vitro gas production (IVGP) at five different ensiling times of 0, 30, 45, 60 and 90 days. The physical quality (color, smell, softness characteristics) of wet rice straw was improved with urea and molasses treatment. Treatments T5 was found better as there was no fungal growth till 90 days of ensiling. The addition of urea and molasses improved the physical quality, nutritive value and preservation quality of wet rice straw. Urea and molasses treated and ensiled (T5) straw showed better color, nutritional quality, softness and longer preservation capacity compared with all treatments followed by T4, T2 and T3. The crude protein (CP) content was increased (P<0.05) but the crude fibre (CF), dry matter (DM), ash contents and ether extract (EE) were decreased (P<0.05) in all of the treatments (T2, T3, T4 and T5) compared to control (T1). The OMD, IVGP and ME contents were increased in all of the treatments (T2, T3, T4 and T5) compared to control (T1). The highest OMD, IVGP and ME values were observed in treatment T5 and the lowest values were in control (T1) Through the Consideration of all the chemical and physical properties, among all of the treatments, 10% urea and 5% molasses are found acceptable for the preservation of rice straw. Thus, environment friendly and cost effective feed can be formulated.


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