Performance of an Escherichia coli phytase expressed in Lactococcus lactis on nutrient retention, bone traits and intestinal morphology in broiler chickens

2020 ◽  
Vol 104 (3) ◽  
pp. 909-917
Author(s):  
Masoud Mohammadi Ziarat ◽  
Hassan Kermanshahi ◽  
Hassan Nasiri Mogaddam ◽  
Reza Majidzadeh Heravi
Keyword(s):  
Escherichia Coli ◽  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Nutrient Retention ◽  
Intestinal Morphology

scholarly journals Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3293
Author(s):  
Monika Bogusławska-Tryk ◽  
Ewa Ziółkowska ◽  
Anna Sławińska ◽  
Maria Siwek ◽  
Joanna Bogucka
Keyword(s):  
Small Intestine ◽  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Intestinal Morphology ◽  
Intestinal Histology ◽  
In Ovo ◽  
Final Body Weight ◽  
Sterile Saline ◽  
Lactococcus Lactis Subsp ◽  
Bioactive Substance

The aim of the study was to determine the effect of probiotics, prebiotics and synbiotics administered in ovo on selected morphological parameters of the small intestine (duodenum, jejunum, ileum) in broiler chickens (Ross 308) and native chickens (Green-legged Partridge, GP). On the 12th day of embryonic development (the incubation period), an aqueous solution of a suitable bioactive substance was supplied in ovo to the egg’s air cell: probiotic—Lactococcus lactis subsp. cremoris (PRO), prebiotic—GOS, galacto-oligosaccharides (PRE) or symbiotic—GOS + Lactococcus lactis subsp. cremoris (SYN). Sterile saline was injected into control (CON) eggs. After hatching, the chicks were placed in pens (8 birds/pen, 4 replicates/group) and housed for 42 days. On the last day of the experiment, all birds were individually weighed and slaughtered. Samples for histological analysis were taken directly after slaughter from three sections of the small intestine. In samples from the duodenum, jejunum and ileum, the height and width of the intestinal villi (VH) were measured and their area (VA) was calculated, the depth of the intestinal crypts (CD) was determined, the thickness of the muscularis was measured and the ratio of the villus height to the crypt depth (V/C) was calculated. On the basis of the obtained data, it can be concluded that the applied substances administered in ovo affect the production parameters and intestinal morphology in broiler chickens and GP. The experiment showed a beneficial effect of in ovo stimulation with a prebiotic on the final body weight of Ross 308 compared to CON, while the effect of the administered substances on the intestinal microstructure is not unequivocal. In GP, the best effect in terms of villi height and V/C ratio was found in the in ovo synbiotic group. Taking into account the obtained results, it can be concluded that chickens of different genotypes react differently to a given substance; therefore, the substances should be adapted to the genotype.

Effect of in ovo injected prebiotics and synbiotics on the caecal fermentation and intestinal morphology of broiler chickens

2017 ◽  
Vol 57 (9) ◽  
pp. 1884 ◽  
Author(s):  
D. Miśta ◽  
B. Króliczewska ◽  
E. Pecka-Kiełb ◽  
V. Kapuśniak ◽  
W. Zawadzki ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Intestinal Morphology ◽  
Jejunal Mucosa ◽  
In Ovo ◽  
Lactococcus Lactis Subsp ◽  
Crypt Depth ◽  
Molar Proportion ◽  
Depth Ratio ◽  
And Performance

Manipulations of the intestinal microbiota composition may improve the health and performance of chickens. In ovo technology allows the administration of a bioactive substance to enter directly into the incubating egg. The objective of the present study was to investigate the effect of in ovo administered prebiotics or synbiotics on microbial activity products in the caeca and the development of the small intestine of broiler chickens. Ross 308 male chickens hatched from eggs injected in ovo with prebiotics or synbiotics were used in this study. Five experimental groups were formed: C (Control) – injected with 0.9% NaCl, the Pre-1 and Pre-2 groups – injected with prebiotics: inulin or Bi2tos, respectively, and the Syn-1 and Syn-2 groups – injected with synbiotics: inulin with Lactococcus lactis subsp. lactis IBB SL1 or Bi2tos with Lactococcus lactis subsp. cremoris IBB SC1, respectively. At the age of 7, 21 and 35 days, 10 chicks of each group were randomly selected, weighed and slaughtered, and the jejunal samples were collected for histological examinations, whereas caecal samples were collected to analyse the end products of microbial fermentation. Synbiotic treatment increased bodyweight, as observed in the Syn-1 group (P < 0.05). The propionate molar proportion was highest in the groups treated with synbiotics, especially in the Syn-1 group (P < 0.01). Furthermore, the molar proportion of acetate was also lowest in the Syn-1 group (P < 0.05). In ovo synbiotics treatment increased the villus length : crypt depth ratio in the jejunal mucosa, which might improve nutrient absorption and contribute to the increased weight of chickens. These effects suggest that the in ovo administration of synbiotics may be an effective method to increase bodyweight, improve the short-chain fatty acid caecal profile and increase the villus length : crypt depth ratio in the jejunal mucosa. These effects were more pronounced in the Syn-1 group than the Syn-2 group.

scholarly journals Research Note: Application of an Escherichia coli spray challenge model for neonatal broiler chickens

2021 ◽  
Vol 100 (4) ◽  
pp. 100988
Author(s):  
C.M. Selby ◽  
B.D. Graham ◽  
L.E. Graham ◽  
K.D. Teague ◽  
B.M. Hargis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Research Note
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