The impact of in ovo injection of l ‐arginine on hatchability, immune system and caecum microflora of broiler chickens

2019 ◽  
Vol 104 (1) ◽  
pp. 178-185 ◽  
Author(s):  
Somayeh Omidi ◽  
Marziyeh Ebrahimi ◽  
Hossein Janmohammadi ◽  
Gholamali Moghaddam ◽  
Zolfaghar Rajabi ◽  
...  
Keyword(s):  
Immune System ◽  
Broiler Chickens ◽  
In Ovo ◽  
In Ovo Injection ◽  
The Impact

Effects of in ovo injection of Astragalus kahericus polysaccharide on early growth, carcass weights and blood metabolites in broiler chickens

2021 ◽  
pp. 1-7
Author(s):  
Hussin H. El-Fakhrany ◽  
Zenat A. Ibrahim ◽  
Elwy A. Ashour ◽  
Ali Osman ◽  
Mahmoud Alagawany
Keyword(s):  
Broiler Chickens ◽  
Early Growth ◽  
Blood Metabolites ◽  
In Ovo ◽  
In Ovo Injection

scholarly journals Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 476
Author(s):  
Marianna Szczypka ◽  
Agnieszka Suszko-Pawłowska ◽  
Maciej Kuczkowski ◽  
Michał Gorczykowski ◽  
Magdalena Lis ◽  
...  
Keyword(s):  
Immune System ◽  
Lactococcus Lactis ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Lymphocyte Subsets ◽  
Double Positive ◽  
In Ovo ◽  
Lactococcus Lactis Subsp ◽  
Egg Incubation ◽  
Stimulation Of

The effects of in ovo-delivered prebiotics and synbiotics on the lymphocyte subsets of the lymphoid organs in non-immunized 7-day-old broiler chickens and in non-immunized, sheep red blood cells (SRBC)-immunized, and dextran (DEX)-immunized 21- and 35-day-old birds were studied. The substances were injected on the 12th day of egg incubation: Prebiotic1 group (Pre1) with a solution of inulin, Prebiotic2 group (Pre2) with a solution of Bi2tos (non-digestive transgalacto-oligosaccharides), Synbiotic1 group (Syn1) with inulin and Lactococcus lactis subsp. lactis IBB SL1, and Synbiotic2 group (Syn2) with Bi2tos and Lactococcus lactis subsp. cremoris IBB SC1. In 7-day-old chicks, a decrease in T splenocytes was noticed in all groups. The most pronounced effect in 21- and 35-day-old birds was an increase in TCRγδ+ cells in Syn1 and Syn2 groups. A decrease in bursal B cells was observed in DEX-immunized Pre1 group (21-day-old birds), and in the Syn1 group in non-immunized and SRBC-immunized 35-day-old birds. An increase in double-positive lymphocytes was observed in Pre1 (35-day-old birds) and Pre2 (immunized 21-day-old birds) groups. In Pre1 and Syn1 groups (21- and 35-day-old), an increase in B splenocytes and a decrease in T splenocytes were observed. We concluded that Syn1 was the most effective in the stimulation of the chicken immune system.

scholarly journals Splenic Gene Expression Signatures in Slow-Growing Chickens Stimulated in Ovo with Galactooligosaccharides and Challenged with Heat

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 474 ◽  
Author(s):  
Elzbieta Pietrzak ◽  
Aleksandra Dunislawska ◽  
Maria Siwek ◽  
Marco Zampiga ◽  
Federico Sirri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Broiler Chickens ◽  
Physiological Saline ◽  
Negative Effects ◽  
In Ovo ◽  
Stress Related Genes ◽  
The Impact ◽  
Slow Growing ◽  
Resistance To Heat

Galactooligosaccharides (GOS) that are delivered in ovo improve intestinal microbiota composition and mitigate the negative effects of heat stress in broiler chickens. Hubbard hybrids are slow-growing chickens with a high resistance to heat. In this paper, we determined the impact of GOS delivered in ovo on slow-growing chickens that are challenged with heat. The experiment was a 2 × 2 × 2 factorial design. On day 12 of incubation, GOS (3.5 mg/egg) was delivered into the egg (n = 300). Controls (C) were mock-injected with physiological saline (n = 300). After hatching, the GOS and C groups were split into thermal groups: thermoneutral (TN) and heat stress (HS). HS (30 °C) lasted for 14 days (days 36–50 post-hatching). The spleen (n = 8) was sampled after acute (8.5 h) and chronic (14 days) HS. The gene expression of immune-related (IL-2, IL-4, IL-6, IL-10, IL-12p40, and IL-17) and stress-related genes (HSP25, HSP90AA1, BAG3, CAT, and SOD) was detected with RT-qPCR. Chronic HS up-regulated the expression of the genes: IL-10, IL-12p40, SOD (p < 0.05), and CAT (p < 0.01). GOS delivered in ovo down-regulated IL-4 (acute p < 0.001; chronic p < 0.01), IL-12p40, CAT and SOD (chronic p < 0.05). The obtained results suggest that slow-growing hybrids are resistant to acute heat and tolerant to chronic heat, which can be supported with in ovo GOS administration.

scholarly journals Injection of Raffinose Family Oligosaccharides at 12 Days of Egg Incubation Modulates the Gut Development and Resistance to Opportunistic Pathogens in Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 592
Author(s):  
Katarzyna Stadnicka ◽  
Joanna Bogucka ◽  
Magdalena Stanek ◽  
Radomir Graczyk ◽  
Krzysztof Krajewski ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Lupinus Luteus ◽  
Gut Development ◽  
Production Environment ◽  
Opportunistic Pathogens ◽  
Raffinose Family Oligosaccharides ◽  
Feed Conversion ◽  
In Ovo ◽  
Blood Profile ◽  
The Impact

The aim was to investigate the impact of an automatic in ovo injection of the raffinose family oligosaccharides (RFO) extracted from the seeds of Lupinus luteus L, on the chicken performance and resistance in a production environment. At day 12 of incubation, a total of 57,900 eggs (Ross 308) were divided into two groups: 1/ Control, injected with 0.9% NaCl and 2/ RFO group, injected with 1.9 mg/egg of the lupin seed extract, dissolved in 0.2 mL NaCl. The performance parameters, biochemical indices (lipid profile, hepatic parameters), gut histomorphology and duodenum structure, oxidative stability of the meat and microbiological counts of the major commensal microbiota species were analyzed. Mortality, body weight, and feed conversion ratio (FCR) were not affected. By day 42, several health indices were improved with RFO and were reflected in a beneficial lipid blood profile, increased villi surface and better combating opportunistic pathogens through reduction of Clostridia and decreased coccidia counts. The RFO increased meat oxidation, but only at the beginning of the storage. The RFO sourced from local legumes can be considered a promising prebiotic for broiler chickens. In ovo delivery of prebiotics and/or synbiotics should be further optimized as an important strategy for the earliest possible modulation of chicken resistance.

Post hatch recovery of a probiotic Enterococcus faecium strain in the yolk sac and intestinal tract of broiler chickens after in ovo injection

2019 ◽  
Vol 366 (7) ◽  
Author(s):  
Line Skjøt-Rasmussen ◽  
Dorthe Sandvang ◽  
Alfred Blanch ◽  
Jette Mundus Nielsen ◽  
Tina Styrishave ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Broiler Chickens ◽  
Intestinal Tract ◽  
Yolk Sac ◽  
In Ovo ◽  
In Ovo Injection ◽  
Faecium Strain

scholarly journals Effects of in ovo injection with selenium on immune and antioxidant responses during experimental necrotic enteritis in broiler chickens

2014 ◽  
Vol 93 (5) ◽  
pp. 1113-1121 ◽  
Author(s):  
S.H. Lee ◽  
H.S. Lillehoj ◽  
S.I. Jang ◽  
M.S. Jeong ◽  
S.Z. Xu ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Necrotic Enteritis ◽  
In Ovo ◽  
Antioxidant Responses ◽  
In Ovo Injection

scholarly journals In ovo administration of CpG ODN induces expression of immune response genes in neonatal chicken spleen

2017 ◽  
Vol 61 (4) ◽  
pp. 451-458 ◽  
Author(s):  
Joanna Sajewicz-Krukowska ◽  
Monika Olszewska-Tomczyk ◽  
Katarzyna Domańska-Blicharz
Keyword(s):  
Immune Response ◽  
Immune System ◽  
High Dose ◽  
Cpg Odn ◽  
In Ovo ◽  
Chicken Spleen ◽  
Class B ◽  
Synthetic Ligand ◽  
The Impact ◽  
Experimental Group

AbstractIntroduction:Due to their immunostimulatory properties TLR ligands are used prophylactically to protect against a variety of viral and bacterial pathogens in mammals. Knowledge of the molecular and functional aspects of TLRs is essential for a better understanding of the immune system and resistance to diseases in birds. For that reason, this study attempted to determine the impact of TLR21 stimulation by its synthetic ligand (CpG ODN, class B) on the chicken immune system.Material and Methods:Sixty embryonated chicken eggs were randomly allocated into three groups (control and two experimental groups). On day 18 of embryonic development, chickens in one experimental group were administeredin ovoa low dose of CpG ODN and the birds of the second experimental group were given a high dose of the ligand. Spleens were collected at 1, 2, 5, and 10 days post-hatching (dph) for analysis of IFN-α, IFN-β, IFN-γ, IL-6, and IL-10 expression using qRT-PCR.Results:Significant differences were observed in mRNA expression levels of all the measured cytokines associated with the modulation and regulation of the immune response at different time points.Conclusion:The obtained data clearly demonstrate that immune response induction takes place afterin ovoadministration of class B CpG ODN, and that the ligand has the ability to induce cytokine responses in neonatal chicken spleen.

scholarly journals Effects of in ovo injection of prebiotics and synbiotics on the productive performance and microstructural features of the superficial pectoral muscle in broiler chickens

2019 ◽  
Vol 98 (10) ◽  
pp. 5157-5165 ◽  
Author(s):  
A Dankowiakowska ◽  
J Bogucka ◽  
A Sobolewska ◽  
S Tavaniello ◽  
G Maiorano ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Pectoral Muscle ◽  
Productive Performance ◽  
In Ovo ◽  
In Ovo Injection
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