Bta‐miR‐152 affects intracellular triglyceride content by targeting the UCP3 gene

2019 ◽  
Vol 103 (5) ◽  
pp. 1365-1373 ◽  
Author(s):  
Binglei Shen ◽  
Shuo Han ◽  
Yuxuan Wang ◽  
Zhuonina Yang ◽  
Ziwen Zou ◽  
...  
Keyword(s):  
Ucp3 Gene ◽  
Triglyceride Content ◽  
Intracellular Triglyceride

NS5ATP6 modulates intracellular triglyceride content through FGF21 and independently of SIRT1 and SREBP1

2016 ◽  
Vol 475 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Zhongshu Li ◽  
Shenghu Feng ◽  
Li Zhou ◽  
Shunai Liu ◽  
Jun Cheng
Keyword(s):  
Triglyceride Content ◽  
Intracellular Triglyceride

Anti-obesity Effect of Gold Nanoparticles from Dendropanax morbifera Léveille by Suppression of Triglyceride Synthesis and Downregulation of PPARγ and CEBPα Signaling Pathways in 3T3-L1 Mature Adipocytes and HepG2 Cells

2020 ◽  
Vol 16 (2) ◽  
pp. 196-203 ◽  
Author(s):  
Myoung Hi Yi ◽  
Shakina Yesmin Simu ◽  
Sungeun Ahn ◽  
Verónica Castro Aceituno ◽  
Chao Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Fatty Liver ◽  
Lipid Accumulation ◽  
Hepg2 Cells ◽  
Cellular Level ◽  
Dendropanax Morbifera ◽  
Immunoblotting Assay ◽  
Triglyceride Content ◽  
20 Nm ◽  
Proliferation Assays

Background: Biosynthesis of gold nanoparticles from medicinal plants has become an interesting strategy in biomedical research due to its exclusive properties including less toxic cellular level through its ecofriendly biological function. Objective: To examine the anti-lipid accumulation effect of spherical gold nanoparticles (size 10-20 nm) synthesized from Dendropanax morbifera Léveille (D-AuNPs) in both 3T3-L1 and HepG2 cells. Method: 3T3-L1 preadipocytes and HepG2 hepatocytes were stimulated with cocktail media to generate obese and fatty liver disease models. Cell cytotoxicity and cell proliferation assays were performed in adipocytes at different stages of growth. An anti-lipid accumulation assay was performed in 3T3-L1 obese and HepG2 fatty liver models using different doses of D-AuNPs. Expression of adipogenic genes of PPARγ, CEBPα, Jak2, STAT3, and ap2 and hepatogenic genes PPARα, FAS, and ACC was measured by real-time PCR. In addition, protein expression of PPARγ and CEBPα was evaluated by immunoblotting assay. Result: We found that D-AuNPs (size 10–20 nm) at concentrations up to 100 µg/ml were nontoxic to 3T3-L1 and HepG2 at post-confluent and mature stages. In addition, pretreatment of D-AuNPs at post-confluent stage reduced triglyceride content. In addition, the adipogenesis process was negatively controlled by D-AuNPs, with downregulated PPARγ, CEBPα, Jak2, STAT3, and ap2 expression in 3T3-L1 cells and FAS and ACC levels in HepG2 cells. Conclusion: These data indicated that D-AuNPs exert antiadipogenic properties. We hypothesize that Dendropanax contains a large amount of phenolic compound that coats the surface of gold nanoparticles and has the ability to reduce the excess amount of lipid in both cell lines.

scholarly journals Influence of xanthine oxidoreductase inhibitor, topiroxostat, on body weight of diabetic obese mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takashi Nakamura ◽  
Mai Nampei ◽  
Takayo Murase ◽  
Etsuko Satoh ◽  
Seigo Akari ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Ketone Body ◽  
The Body ◽  
Xanthine Oxidoreductase ◽  
Diabetic Mellitus ◽  
Triglyceride Content ◽  
Xanthine Oxidoreductase Inhibitor ◽  
Single Regression ◽  
Muscle Triglyceride

AbstractPlasma xanthine oxidoreductase (XOR) activity is high in metabolic disorders such as diabetic mellitus, obesity, or overweight. Thus, this study investigated whether the XOR inhibitor, topiroxostat, affected body weight. Male db/db mice were fed standard diets with or without topiroxostat for 4 weeks. Body weight and food intake were constantly monitored, along with monitoring plasma biochemical markers, including insulin and XOR activity. Additionally, hepatic hypoxanthine and XOR activity were also documented. Single regression analysis was performed to determine the mechanism. Topiroxostat treatment suppressed weight gain relative to the vehicle without any impact on food intake. However, the weight of fat pads and hepatic and muscle triglyceride content did not change. Topiroxostat decreased the plasma uric acid and increased hepatic hypoxanthine in response to the inhibition of XOR activity. Plasma ketone body and free fatty acid were also increased. Moreover, fat weight was weakly associated with plasma XOR activity in the diabetic state and was negatively associated with ketone body by topiroxostat. These results suggested that topiroxostat amplified the burning of lipids and the salvage pathway, resulting in predisposing the body toward catabolism. The inhibition of plasma XOR activity may contribute to weight loss.

scholarly journals Validation of a Method Based on Triglycerides for the Detection of Low Percentages of Hazelnut Oil in Olive Oil by Column Liquid Chromatography

2007 ◽  
Vol 90 (5) ◽  
pp. 1346-1353 ◽  
Author(s):  
Diego L García-González ◽  
María Viera-Macías ◽  
Ramón Aparicio-Ruiz ◽  
Maria T Morales ◽  
Ramón Aparicio
Keyword(s):  
Liquid Chromatography ◽  
Olive Oil ◽  
Carbon Number ◽  
Hazelnut Oil ◽  
Equivalent Carbon Number ◽  
Experimental Conditions ◽  
Mathematical Algorithm ◽  
Relative Standard ◽  
Triglyceride Content ◽  
The Difference

Abstract The difference between theoretical and empirical triglyceride content is a powerful tool to detect the presence of any vegetable oil in olive oil. The current drawback of the method is the separation between equivalent carbon number ECN42 compounds, which affects the reliability of the method and, hence, its cutoff limit. The determination of the triglyceride profile by liquid chromatography using propionitrile as the mobile phase has recently been proposed to improve their quantification, together with a mathematical algorithm whose binary response determines the presence or absence of hazelnut oil. Twenty-one laboratories from 9 countries participated in an interlaboratory study to evaluate the performance characteristics of the whole analytical method. Participants analyzed 12 samples in duplicate, split into 3 intercomparison studies. Statistically significant differences due to the experimental conditions were found in some laboratories, which were detected as outliers by use of Cochran's and Grubbs' tests. The relative standard deviations (RSD) for repeatability and reproducibility were determined following the AOAC Guidelines for Collaborative Studies. The analytical properties of the method were determined by means of the sensitivity (0.86), selectivity (0.94), and reliability (72) for a cutoff limit of 8 (probability 94).

Downregulation of interleukin 32 reduce intracellular triglyceride levels in liver spheroids

2021 ◽  
Vol 331 ◽  
pp. e143
Author(s):  
K. Sasidharan ◽  
A.M. Caddeo ◽  
O. Jamialahmadi ◽  
G. Baselli ◽  
F. Malvestiti ◽  
...  
Keyword(s):  
Intracellular Triglyceride

Antidiabetic agent pioglitazone enhances adipocyte differentiation of 3T3-F442A cells

1993 ◽  
Vol 264 (6) ◽  
pp. C1600-C1608 ◽  
Author(s):  
T. Sandouk ◽  
D. Reda ◽  
C. Hofmann
Keyword(s):  
Cell Differentiation ◽  
Energy Homeostasis ◽  
Combined Treatment ◽  
Transcript Abundance ◽  
Dependent Manner ◽  
Antidiabetic Agent ◽  
Triglyceride Accumulation ◽  
Genes Encoding ◽  
Lower Plasma Glucose ◽  
Triglyceride Content

Adipocytes play an important role in normal physiology as a major site for systemic energy homeostasis. In disorders such as diabetes, adipocyte function is markedly altered. In this study, we investigated the effect of pioglitazone, a novel antidiabetic agent known to lower plasma glucose in animal models of diabetes mellitus, on cellular differentiation and expression of adipose-specific genes. Treatment of confluent 3T3-F442A preadipocyte cultures for 7 days with pioglitazone (Pio; 1 microM) and insulin (Ins; 0.17 microM) resulted in > 95% cell differentiation into lipid-accumulating adipocytes in comparison with 60-80% cell differentiation by treatment with either agent alone. Analysis of triglyceride accumulation showed increases of triglyceride content over time above untreated preadipocytes by treatment of the cells with Ins, Pio, and especially with Ins + Pio. Basal glucose transport, as measured by cellular uptake of 2-deoxy-D-[14C]glucose, was likewise enhanced in a time-dependent manner by treatment of preadipocytes with Ins, Pio, or Ins + Pio, such that a synergistic effect resulted from the combined treatment with both agents. It was further determined that RNA transcript abundance for genes encoding glucose transporters GLUT-1 and GLUT-4, as well as the adipose-specific genes encoding adipsin and aP2, were increased by the Ins, Pio, or Ins + Pio treatment. Taken together, these findings indicate that pioglitazone is a potent adipogenic agent. By promoting differentiation, this agent may move cells into a state active for glucose uptake, storage, and metabolism.

Hepatic Leptin Signalling Modulates ApoB Containing Lipoprotein Size and Triglyceride Content

2012 ◽  
Vol 36 (5) ◽  
pp. S65-S66
Author(s):  
Frank K. Huynh ◽  
Ursula H. Neumann ◽  
Ying Wang ◽  
Brian Rodrigues ◽  
Timothy J. Kieffer ◽  
...  
Keyword(s):  
Triglyceride Content ◽  
Lipoprotein Size
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