Changes in the regulation and activity of glutathione redox system, and lipid peroxidation processes in short-term aflatoxin B1 exposure in liver of laying hens

2018 ◽  
Vol 102 (4) ◽  
pp. 947-952 ◽  
Author(s):  
M. Erdélyi ◽  
K. Balogh ◽  
C. Pelyhe ◽  
B. Kövesi ◽  
M. Nakade ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aflatoxin B1 ◽  
Laying Hens ◽  
Redox System ◽  
Short Term ◽  
Glutathione Redox System ◽  
Peroxidation Processes ◽  
Glutathione Redox

scholarly journals Lack of Dose- and Time-Dependent Effects of Aflatoxin B1 on Gene Expression and Enzymes Associated with Lipid Peroxidation and the Glutathione Redox System in Chicken

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 84 ◽  
Author(s):  
Benjámin Kövesi ◽  
Mátyás Cserháti ◽  
Márta Erdélyi ◽  
Erika Zándoki ◽  
Miklós Mézes ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aflatoxin B1 ◽  
Control Diet ◽  
Dose Range ◽  
Thiobarbituric Acid ◽  
Redox System ◽  
Time Dependent ◽  
Treatment Groups ◽  
Glutathione Redox System ◽  
Glutathione Redox

Effects of aflatoxin B1 (AFB1) on lipid peroxidation and glutathione system were investigated in chicken liver. In a three-week feeding trial, different doses (<1.0 μg/kg (control diet), 17.0 µg (diet A1), 92.0 µg (diet A2), and 182.0 µg (diet A3) AFB1 kg/feed) were used. Markers of lipid peroxidation, conjugated dienes and trienes showed higher values in A3, while amounts of thiobarbituric acid reactive substances were increased in the A1 group at day 21. Glutathione content was lower at day 14 in Group A2. Glutathione peroxidase 4 activity was increased at days 7 and 21 in the A3 group but reduced in the A2 and A3 groups at day 14. The GPX4 gene was downregulated at day 7 in the A2 group, but overregulated at days 14 and 21, and at day 14 in the A3 group. GSS was downregulated at day 14 in the A1 group but overregulated at day 21 in A1 and A2 groups. GSR was downregulated at days 7 and 21 in all treatment groups, but on day 14, induction was observed in the A3 group. The results indicated that AFB1 did not induce dose- or time-dependent effects on the glutathione redox system and its encoding genes at the dose range used, which means that oxidative stress is not the primary effect of AFB1 toxicity.

scholarly journals Individual and Combined Effects of Aflatoxin B1 and Sterigmatocystin on Lipid Peroxidation and Glutathione Redox System of Common Carp Liver

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 109
Author(s):  
Benjamin Kövesi ◽  
Szabina Kulcsár ◽  
Zsolt Ancsin ◽  
Erika Zándoki ◽  
Márta Erdélyi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Common Carp ◽  
Aflatoxin B1 ◽  
Regulatory Gene ◽  
Redox System ◽  
Glutathione Biosynthesis ◽  
Glutathione Redox System ◽  
One Year ◽  
Encoding Genes ◽  
Glutathione Redox

The purpose of the study was to evaluate the short-term effects of aflatoxin B1 (AFB1 100 µg/kg feed) and sterigmatocystin (STC 1000 μg/kg feed) exposure individually and in combination (100 μg AFB1 + 1000 μg STC/kg feed) on the parameters of lipid peroxidation and glutathione redox system both in biochemical and gene expression levels in one-year-old common carp. Lipid peroxidation parameters were slightly affected, as significant differences were observed only in conjugated diene and triene concentrations. Reduced glutathione content decreased more markedly by STC than AFB1 or AFB1+STC, but glutathione peroxidase activity did not change. Expression of gpx4a, gpx4b, gss, and gsr genes was down-regulated due to STC compared to AFB1 or AFB1+STC, while an induction was found as effect of AFB1+STC in the case of gpx4a, but down-regulation for gpx4b as compared to AFB1. Expression of the glutathione biosynthesis regulatory gene, gss, was higher, but glutathione recycling enzyme encoding gene, gsr, was lower as an effect of AFB1+STC compared to AFB1. These results are supported by the changes in the expression of transcription factors encoding genes, nrf2, and keap1. The results revealed that individual effects of AFB1 and STC on different parameters are synergistic or antagonistic in multi-toxin treatment.

scholarly journals Effects of Fusarium Mycotoxin Exposure on Lipid Peroxidation and Glutathione Redox System in the Liver of Laying Hens

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1313
Author(s):  
Szabina Kulcsár ◽  
Benjámin Kövesi ◽  
Krisztián Balogh ◽  
Erika Zándoki ◽  
Zsolt Ancsin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Laying Hens ◽  
Fumonisin B1 ◽  
Redox System ◽  
Fusarium Mycotoxins ◽  
Glutathione Redox System ◽  
Glutathione Redox ◽  
Gene Expression Levels

It has been proven by several studies that Fusarium mycotoxins induce oxidative stress in animals, consequently inducing lipid peroxidation, which the glutathione system can neutralize. A short-term (3-day) in vivo feeding trial was performed with laying hens using a double dose of the EU recommendation for mycotoxin contamination (T-2 toxin 0.5 mg/kg feed; deoxynivalenol (DON) 10 mg/kg feed; fumonisin B1 (FB1) 40 mg/kg feed). Some lipid peroxidation and glutathione redox system parameters and gene expression levels were measured in the liver. The results show that FB1 significantly decreased the reduced glutathione (GSH) content and the activity of glutathione peroxidase (GPx) compared to the control and the two other mycotoxin-treated groups on day 3. Lipid peroxidation was affected by all three mycotoxins. Significantly lower values were observed in the case of conjugated dienes for all of the three mycotoxins and malondialdehyde concentration as an effect of DON on day 3. T-2 toxin and DON upregulated the expression of the GPX4 gene. The results show that Fusarium mycotoxins had different effects at the end of the trial. The FB1 exposure caused a decrease in the glutathione redox markers, while DON decreased the formation of malondialdehyde. The results suggest that the Fusarium mycotoxins investigated individually differently activated the antioxidant defense and caused low-level oxidative stress at the dose applied.

scholarly journals Short-term effects of deoxynivalenol, T-2 toxin, fumonisin B1 or ochratoxin on lipid peroxidation and glutathione redox system and its regulatory genes in common carp (Cyprinus carpio L.) liver

2020 ◽  
Vol 46 (6) ◽  
pp. 1921-1932
Author(s):  
Benjámin Kövesi ◽  
Szabina Kulcsár ◽  
Erika Zándoki ◽  
Judit Szabó-Fodor ◽  
Miklós Mézes ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Glutathione Peroxidase ◽  
Common Carp ◽  
Fumonisin B1 ◽  
Redox System ◽  
Short Term ◽  
Initiation Phase ◽  
Glutathione Redox System ◽  
Antioxidant Defence System ◽  
Glutathione Redox

Abstract The effects of a single oral dose of 1.82 mg kg−1 bw of T-2 and HT-2 toxin (T-2), 1.75 mg kg−1 bw deoxynivalenol (DON) and 15-acetyl DON, 1.96 mg kg−1 bw fumonisin B1 (FB1) or 1.85 mg kg−1 bw ochratoxin A (OTA) were investigated in common carp juveniles on lipid peroxidation, the parameters of the glutathione redox system including the expression of their encoding genes in a short-term (24 h) experiment. Markers of the initiation phase of lipid peroxidation, conjugated dienes, and trienes, were slightly affected by DON and OTA treatment at 16-h sampling. The termination marker, malondialdehyde, concentration increased only as an effect of FB1. Glutathione content and glutathione peroxidase activity showed significantly higher levels in the T-2 and FB1 groups at 8 h, and in the DON and FB1 groups at 16 h. The expression of glutathione peroxidase genes (gpx4a, gpx4b) showed a dual response. Downregulation of gpxa was observed at 8 h, as the effect of DON, FB1, and OTA, but an upregulation in the T-2 group. At 16 h gpx4a upregulated as an effect of DON, T-2, and FB1, and at 24 h in the DON and T-2 groups. Expression of gpx4b downregulated at 8 h, except in the T-2 group, and upregulation observed as an effect of T-2 at 24 h. The lack of an increase in the expression of nrf2, except as the effect of DON at 8 h, and a decrease in the keap1 expression suggests that the antioxidant defence system was activated at gene and protein levels through Keap1–Nrf2 independent pathways.

Age-dependent effects of short-term exposure of T-2 toxin or deoxynivalenol on lipid peroxidation and glutathione redox system in broiler chickens

2018 ◽  
Vol 11 (4) ◽  
pp. 611-624 ◽  
Author(s):  
Cs. Pelyhe ◽  
B. Kövesi ◽  
J. Szabó-Fodor ◽  
E. Zándoki ◽  
M. Erdélyi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lipid Peroxidation ◽  
Age Groups ◽  
Redox System ◽  
Glutathione Synthetase ◽  
Short Term ◽  
Glutathione Redox System ◽  
Short Term Exposure ◽  
Age Dependent ◽  
Glutathione Redox

Purpose of this study was to investigate the age-dependent, short-term effects of T-2 toxin (5.77 mg T-2 and 1.40 mg HT-2 toxin/kg feed) or deoxynivalenol (DON) (4.86 mg DON and 1.39 mg 15-acetyl-DON/kg feed) in one and three weeks old broiler chicken to observe the changes in parameters of lipid peroxidation, glutathione redox system, and expression of genes related to glutathione redox system in the first 24 h of mycotoxin exposure. Glutathione-redox system responsed to T-2 toxin exposure in both age groups for T-2 toxin in the first 8 h of exposure, while a reactivation was observed in the 3-week-old group after 20 h, although lipid peroxidation did not change significantly. DON did not alter these parameters, only at gene expression level. Gene expression of phospholipid hydroperoxide glutathione peroxidase (GPX4) showed minor, but significant, changes in both age- and mycotoxin exposure groups. Glutathione reductase (GSR) showed a dual response for the mycotoxin exposure, which was not consequent in either age groups, or treatments. Glutathione synthetase (GSS) showed a decreasing tendency in the younger animals while in the older group elevating tendency was observed as effect of both mycotoxins. Time, treatment and their combined effect also showed relation with the changes in the parameters.

scholarly journals Combined effects of aflatoxin B1 and deoxynivalenol on the expression of glutathione redox system regulatory genes in common carp

2020 ◽  
Vol 104 (5) ◽  
pp. 1531-1539
Author(s):  
Benjamin Kövesi ◽  
Csilla Pelyhe ◽  
Erika Zándoki ◽  
Miklós Mézes ◽  
Krisztián Balogh
Keyword(s):  
Common Carp ◽  
Aflatoxin B1 ◽  
Redox System ◽  
Regulatory Genes ◽  
Combined Effects ◽  
Glutathione Redox System ◽  
Glutathione Redox
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