scholarly journals Influence of outdoor rearing and oleic acid supplementation on lipid characteristics of muscle and adipose tissues from obese Alentejano pigs

2017 ◽  
Vol 102 (2) ◽  
pp. e578-e590 ◽  
Author(s):  
J. M. Martins ◽  
A. Albuquerque ◽  
J. A. Neves ◽  
A. B. Freitas ◽  
R. Charneca ◽  
...  
2017 ◽  
Vol 85 (12) ◽  
Author(s):  
Sabrina J. Nolan ◽  
Man Shun Fu ◽  
Isabelle Coppens ◽  
Arturo Casadevall

ABSTRACT Many microbes exploit host cellular lipid droplets during the host-microbe interaction, but this phenomenon has not been extensively studied for fungal pathogens. In this study, we analyzed the role of lipid droplets during the interaction of Cryptococcus neoformans with macrophages in the presence and the absence of exogenous lipids, in particular, oleate. The addition of oleic acid increased the frequency of lipid droplets in both C. neoformans and macrophages. C. neoformans responded to oleic acid supplementation by faster growth inside and outside macrophages. Fungal cells were able to harvest lipids from macrophage lipid droplets. Supplementation of C. neoformans and macrophages with oleic acid significantly increased the rate of nonlytic exocytosis while having no effect on lytic exocytosis. The process for lipid modulation of nonlytic exocytosis was associated with actin changes in macrophages. In summary, C. neoformans harvests lipids from macrophages, and the C. neoformans-macrophage interaction is modulated by exogenous lipids, providing a new tool for studying nonlytic exocytosis.


animal ◽  
2015 ◽  
Vol 9 (10) ◽  
pp. 1721-1730 ◽  
Author(s):  
J.M. Martins ◽  
J.A. Neves ◽  
A. Freitas ◽  
J.L. Tirapicos

Author(s):  
Kim Margarette Nogoy ◽  
Hyoun Ju Kim ◽  
Dong Hoon Lee ◽  
Stephen Smith ◽  
Hyun A Seong ◽  
...  

1981 ◽  
Vol 27 (12) ◽  
pp. 1283-1289 ◽  
Author(s):  
James E. Urban ◽  
W. E. Klopfenstein ◽  
K. Ahmad ◽  
J. D. Baines

Escherichia coli 15T− was grown with glucose, succinic acid, aspartic acid, oleic acid, and oleic plus aspartic acids as carbon sources, and a fatty acid auxotroph derived from 15T− was grown on oleic acid and oleic plus aspartic acids. The doubling time, phospholipid composition, phosphorus content, and the fatty acid composition of the phospholipids of cells in each of the media were determined. In all cases, phosphatidylethanolamine was the major phospholipid present; but with 15T− its concentration was inversely proportional to the doubling time in unsupplemented media. With the auxotroph the phosphatidylethanolamine concentration was essentially unchanged with growth. Total lipid phosphorus was inversely proportional to doubling time, an effect particularly evident with the auxotroph. Without oleic acid supplementation, the major effects of carbon source on fatty acid composition are decreases in the content of palmitoleic acid and increases in the content of cis-9,10-methylene hexadecanoic acid as growth rate decreases. Oleic acid supplementation elevated 18:1 fatty acid content in both 15T− and the auxotroph.


2009 ◽  
Vol 234 (9) ◽  
pp. 1047-1055 ◽  
Author(s):  
Tsz Yan Lam ◽  
Sai Wang Seto ◽  
Alice Lai Shan Au ◽  
Christina Chui Wa Poon ◽  
Rachel Wai Sum Li ◽  
...  

The effects of folic acid (5.7 and 71 μg/kg, 4 weeks) consumption on the β-adrenoceptors (β-ARs)–elicited lipolysis in vitro of the abdominal adipocytes of lean/control (+ m/+ db) and obese/diabetic (+ db/+ db) mice (female) were investigated. β-AR agonists (salbutamol, a β2-AR agonist; BRL 37344 and CGP 12177, β3-AR agonists; adrenaline, a β-AR agonist)–mediated lipolysis, β2-, and β3-ARs protein expression of the adipose tissues after folic acid consumption were evaluated. Our results demonstrate that a smaller magnitude of the basal (spontaneous) and the β-AR agonists–triggered lipolysis was observed in + db/+ db mice, and folic acid supplementation (71 μg/kg) resulted in an improvement of both the baseline and the β-ARs–mediated lipolysis. In controls, a lower β2-and β3-ARs protein expression of the adipose tissues was detected in + db/+ db mice, compared to + m/+ db mice. In both strains fed with folic acid (71 μg/kg), a reduction of β2-AR protein expression was observed compared to the respective controls. In + db/+ db mice, folic acid (5.7 and 71 μg/kg) consumption caused a dose-dependent increase of β3-AR protein expression compared to controls. We demonstrate that lipolysis elicited by β-AR (β2- and β3-ARs) agonists was blunted in + db/+ db mice. Folic acid consumption has significant modulatory effects on β-ARs protein expression and lipolysis.


1989 ◽  
Vol 17 (3) ◽  
pp. 505-506 ◽  
Author(s):  
M. A. BARRADAS ◽  
J. A. CHRISTOFIDES ◽  
J. Y. JEREMY ◽  
D. P. MIKHAILIDIS ◽  
D. E. FRY ◽  
...  

1993 ◽  
Vol 156 (1) ◽  
pp. 24-34 ◽  
Author(s):  
C. Michael Hart ◽  
Sharon P. Andreoli ◽  
Carolyn E. Patterson ◽  
Joe G. N. Garcia

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Adilson Costa ◽  
Aline Siqueira Talarico ◽  
Carla de Oliveira Parra Duarte ◽  
Caroline Silva Pereira ◽  
Ellem Tatiani de Souza Weimann ◽  
...  

Background. Acne is a dermatosis that involves an altered sebum pattern.Objectives. (1) To evaluate if a treatment based on antibiotics (lymecycline) can alter fatty acids contents of the sebum of patients with acne; (2) to evaluate if oral supplementation of fatty acids can interfere with fatty acids contents of the sebum of patients with acne; (3) to evaluate if there is any interaction in fatty acids contents of the sebum of patients with acne when they use both antibiotics and oral supplementation of fatty acids.Methods. Forty-five male volunteers with inflammatory acne vulgaris were treated with 300 mg of lymecycline per day, with 540 mg ofγ-linolenic acid, 1,200 mg of linoleic acid, and 510 mg of oleic acid per day, or with both regimens for 90 days. Every 30 days, a sample of sebum from the forehead was collected for fatty acids’ chromatographic analysis.Results. Twelve fatty acids studied exhibited some kind of pattern changes during the study: C12:0, C14:0, C15:0, C16:1, C18:0, C18:1n9c+C18:1n9t, C18:2n6t, C18:3n6, C18:3n3, C20:1, C22:0, and C24:0.Conclusions. The daily administration of lymecycline and/or specific fatty acids may slightly influence some fatty acids levels present in the sebum of patients with inflammatory acne vulgaris.


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