scholarly journals Mitochondrial function is controlled by melatonin and its metabolites in vitro in human melanoma cells

2021 ◽  
Vol 70 (3) ◽  
Author(s):  
Bernadetta Bilska ◽  
Fiona Schedel ◽  
Anna Piotrowska ◽  
Joanna Stefan ◽  
Michal Zmijewski ◽  
...  
Keyword(s):  
Mitochondrial Function ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells

A fluorometric rapid microassay to identify anti-proliferative compounds for human melanoma cells in vitro

1991 ◽  
Vol 1 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Ch. C. Zouboulis ◽  
C. Garbe ◽  
K. Krasagakis ◽  
S. Krüger ◽  
C. E. Orfanos
Keyword(s):  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells

Effect of prostaglandin A1 on proliferation and telomerase activity of human melanoma cells in vitro

1998 ◽  
Vol 8 (4) ◽  
pp. 323-328 ◽  
Author(s):  
O Franzese ◽  
A Comandini ◽  
E Cannav?? ◽  
R Pepponi ◽  
S Falcinelli ◽  
...  
Keyword(s):  
Telomerase Activity ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
Prostaglandin A1

NUCLEAR DAMAGE INDUCED BY LIPOSOMES CONTAINING FITC-LABELLED SAPORIN ON HUMAN MELANOMA CELLS IN VITRO

2001 ◽  
Vol 11 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Anna Poma ◽  
Patrizia Cesare ◽  
Giordana Marcozzi ◽  
Laura Spanò
Keyword(s):  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
Nuclear Damage

AMPK activation by GSK621 inhibits human melanoma cells in vitro and in vivo

2016 ◽  
Vol 480 (4) ◽  
pp. 515-521 ◽  
Author(s):  
Lezi Chen ◽  
Quan Chen ◽  
Guosan Deng ◽  
Shifeng Kuang ◽  
Jihong Lian ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Human Melanoma ◽  
Ampk Activation ◽  
Human Melanoma Cells

scholarly journals Treatment of melanoma cells with the synthetic retinoid CD437 induces apoptosis via activation of AP-1 in vitro, and causes growth inhibition in xenografts in vivo.

1996 ◽  
Vol 135 (6) ◽  
pp. 1889-1898 ◽  
Author(s):  
D Schadendorf ◽  
M A Kern ◽  
M Artuc ◽  
H L Pahl ◽  
T Rosenbach ◽  
...  
Keyword(s):  
Cell Death ◽  
Malignant Melanoma ◽  
Programmed Cell Death ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Mrna Levels ◽  
Synthetic Retinoid ◽  
Human Melanoma Cells

Human malignant melanoma is notoriously resistant to pharmacological modulation. We describe here for the first time that the synthetic retinoid CD437 has a strong dose-dependent antiproliferative effect on human melanoma cells (IC50: 5 x 10(-6) M) via the induction of programmed cell death, as judged by analysis of cell morphology, electron microscopical features, and DNA fragmentation. Programmed cell death was preceded by a strong activation of the AP-1 complex in CD437-treated cells as demonstrated by gel retardation and chloramphenicol transferase (CAT) assays. Northern blot analysis showed a time-dependent increase in the expression of c-fos and c-jun encoding components of AP-1, whereas bcl-2 and p53 mRNA levels remained constant. CD437 also exhibited a strong growth inhibitory effect on MeWo melanoma cells in a xenograft model. In tissue sections of CD437-treated MeWo tumors from these animals, apoptotic melanoma cells and c-fos overexpressing cells were colocalized by TdT-mediated deoxyuridine triphosphate-digoxigenin nick end labeling (TUNEL) staining and in situ hybridization. Taken together, this report identifies CD437 as a retinoid that activates and upregulates the transcription factor AP-1, leading eventually to programmed cell death of exposed human melanoma cells in vitro and in vivo. Further studies are needed to evaluate whether synthetic retinoids such as CD437 represent a new class of retinoids, which may open up new ways to a more effective therapy of malignant melanoma.

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