Melatonin suppresses milk fat synthesis by inhibiting themTORsignaling pathway via theMT1 receptor in bovine mammary epithelial cells

2019 ◽  
Vol 67 (3) ◽  
Author(s):  
Yujuan Wang ◽  
Wenli Guo ◽  
Haichao Xu ◽  
Keqiong Tang ◽  
Linsen Zan ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Effects of Insulin on Milk Fat Synthesis by Regulating the Expressions of SREBP1 and mTOR in Bovine Mammary Epithelial Cells

2020 ◽  
Author(s):  
Nan Li ◽  
Peng-Xia Zhang ◽  
Xin Huang ◽  
Hai-Tao Yao ◽  
Dong-Pu Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Blank Group ◽  
Bovine Mammary Epithelial Cells ◽  
Real Time Quantitative Pcr ◽  
Notable Increase ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Due to the complexity of insulin in life activities, the role of insulin in mammalian lactation has not been well explained. To investigate the influence of insulin on milk fat synthesis, bovine mammary epithelial cells (BMECs) were cultured in treatment with insulin. We determined the content of Triglyceride (TG) in cell-free culture medium and found a notable increase in TG secrection. Lipid droplet staining study showed a consistent result. We also used real-time quantitative PCR and western blotting to detect the expression of signaling molecules related to milk fat synthesis. We found that insulin resulted in an obvious increase of SREBP-1, mTOR and lipogenic gene expression compared with the blank group. Taken together, our study reveals that insulin plays a significant role in milk fat synthesis.

scholarly journals MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Juan Liu ◽  
Ping Jiang ◽  
Ambreen Iqbal ◽  
Shaokat Ali ◽  
Zhen Gao ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Target Gene ◽  
Mammary Epithelial Cells ◽  
Cholesterol Content ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Candidate Target Gene ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

AbstractMicroRNAs (miRNAs) are mRNA suppressors that regulate a variety of cellular and physiological processes, including cell proliferation, apoptosis, triglyceride synthesis, fat formation, and lipolysis, by post-transcriptional processing. In previous studies, we isolated and sequenced miRNAs from mammary epithelial cells from Chinese Holstein cows with high and low milk fat percentages. MiR-485 was one of the significantly differentially expressed miRNAs that were identified. In the present study, the relationship between the candidate target gene DTX4 and miR-485 was validated by bioinformatics and real-time fluorescent quantitative PCR (qRT-PCR) and Western blot (WB) analyses in bovine mammary epithelial cells (bMECs). The results indicated that miR-485 negatively regulated the mRNA expression of the target gene DTX4. Furthermore, an shRNA interference vector for the target gene DTX4 was constructed successfully, and it increased the triglyceride content and reduced the cholesterol content of transfected cells. These results suggest that miR-485 may affect the contents of triglycerides (TGs) and cholesterol (CHOL) by targeting the DTX4 gene. This study indicates that miR-485 has a role in regulating milk fat synthesis and that miR-485 targets the DTX4 gene to regulate lipid metabolism in bMECs. These findings contribute to the understanding of the functional significance of miR-485 in milk fat synthesis.

scholarly journals Comparative proteome analysis reveals VPS28 regulates milk fat synthesis through ubiquitylation in bovine mammary epithelial cells

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9542
Author(s):  
Lily Liu ◽  
Qin Zhang
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Proteome Analysis ◽  
Mammary Epithelial ◽  
Absolute Quantitation ◽  
Bovine Mammary Epithelial Cells ◽  
Fat Synthesis ◽  
Vacuolar Protein ◽  
Milk Fat Synthesis

In our previous study, we found that VPS28 (vacuolar protein sorting 28 homolog) could alter ubiquitylation level to regulate milk fat synthesis in bovine primary mammary epithelial cells (BMECs). While the information on the regulation of VPS28 on proteome of milk fat synthesis is less known, we explored its effect on milk fat synthesis using isobaric tags for relative and absolute quantitation assay after knocking down VPS28 in BMECs. A total of 2,773 proteins in three biological replicates with a false discovery rate of less than 1.2% were identified and quantified. Among them, a subset of 203 proteins were screened as significantly down-(111) and up-(92) regulated in VPS28 knockdown BMECs compared with the control groups. According to Gene Ontology analysis, the differentially expressed proteins were enriched in the “proteasome,” “ubiquitylation,” “metabolism of fatty acids,” “phosphorylation,” and “ribosome.” Meanwhile, some changes occurred in the morphology of BMECs and an accumulation of TG (triglyceride) and dysfunction of proteasome were identified, and a series of genes associated with milk fat synthesis, ubiquitylation and proteasome pathways were analyzed by quantitative real-time PCR. The results of this study suggested VPS28 regulated milk fat synthesis was mediated by ubiquitylation; it could be an important new area of study for milk fat synthesis and other milk fat content traits in bovine.

scholarly journals MiR-143 Regulates Milk Fat Synthesis by Targeting Smad3 in Bovine Mammary Epithelial Cells

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1453
Author(s):  
Li Zhang ◽  
Zhang-Qing Wu ◽  
Yu-Juan Wang ◽  
Meng Wang ◽  
Wu-Cai Yang
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Lipid Synthesis ◽  
Mammary Epithelial ◽  
Luciferase Reporter ◽  
Important Indicator ◽  
Bovine Mammary Epithelial Cells ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

Milk fat is the main nutritional component of milk and is also an important indicator for evaluating milk quality. Substantial evidence has implicated miRNAs in the synthesis of milk fat. miR-143 is one of the miRNAs closely related to lipid metabolism. Herein, miR-143 upregulation remarkably promoted the production of lipid droplets and increased the level of intracellular triglyceride (TAG). Meanwhile, miR-143 suppression overtly repressed TAG synthesis and lipid droplet accumulation in bovine mammary epithelial cells (BMECs). At the same time, miR-143 significantly upregulated the genes associated with lipid synthesis, including PPARγ, SCD1, CEBPβ, and SREBP1. To examine the regulatory mechanism of miR-143 in milk fat synthesis, Smad3 was predicted as a new potential miR-143 target gene by TargetScan. Further studies found that miR-143 expression was inversely related to the levels of Smad3 mRNA and protein. Furthermore, luciferase reporter assays confirmed Smad3 to be a miR-143 direct target. Moreover, Smad3 gene silencing significantly increased intracellular TAG level in BMECs. These findings revealed that miR-143 promotes the TAG synthesis in BMECs via increasing the lipid synthesis related gens expression by targeting Smad3. The results of this study can be exploited in devising novel approaches for improving the nutritional value of milk in dairy cows.

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