Melatonin compensates silencing of heat shock protein 70 and suppresses ultraviolet radiation-induced inflammation in human skin ex vivo and cultured keratinocytes

2014 ◽  
Vol 58 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Konrad Kleszczyński ◽  
Stephanie Zwicker ◽  
Stefan Tukaj ◽  
Michael Kasperkiewicz ◽  
Detlef Zillikens ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Ultraviolet Radiation ◽  
Human Skin ◽  
Heat Shock Protein 70 ◽  
Ex Vivo ◽  
Cultured Keratinocytes ◽  
Radiation Induced

scholarly journals The role of heat shock protein 70 (Hsp70) in radiation-induced immunomodulation

2015 ◽  
Vol 368 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Gabriele Multhoff ◽  
Alan G. Pockley ◽  
Thomas E. Schmid ◽  
Daniela Schilling
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Radiation Induced

scholarly journals Expression of Heat Shock Protein 70 in Human Skin Cells as a Photoprotective Function after UV Exposure

2008 ◽  
Vol 20 (4) ◽  
pp. 184 ◽  
Author(s):  
Byoung Hwa Roh ◽  
Dae Hyun Kim ◽  
Moon Kyun Cho ◽  
Young Lip Park ◽  
Kyu Uang Whang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Skin ◽  
Heat Shock Protein 70 ◽  
Uv Exposure ◽  
Skin Cells ◽  
Human Skin Cells

scholarly journals Treatment of Colon and Lung Cancer Patients with ex Vivo Heat Shock Protein 70-Peptide-Activated, Autologous Natural Killer Cells

2004 ◽  
Vol 10 (11) ◽  
pp. 3699-3707 ◽  
Author(s):  
Stefan W. Krause ◽  
Robert Gastpar ◽  
Reinhard Andreesen ◽  
Catharina Gross ◽  
Heidrun Ullrich ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Heat Shock ◽  
Natural Killer Cells ◽  
Heat Shock Protein ◽  
Natural Killer ◽  
Cancer Patients ◽  
Heat Shock Protein 70 ◽  
Ex Vivo ◽  
Killer Cells ◽  
Lung Cancer Patients

scholarly journals Heat shock protein 70 regulates platelet integrin activation, granule secretion and aggregation

2016 ◽  
Vol 310 (7) ◽  
pp. C568-C575 ◽  
Author(s):  
Rachel A. Rigg ◽  
Laura D. Healy ◽  
Marie S. Nowak ◽  
Jérémy Mallet ◽  
Marisa L. D. Thierheimer ◽  
...  
Keyword(s):  
T Cells ◽  
Platelet Aggregation ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Platelet Activation ◽  
Whole Blood ◽  
Heat Shock Protein 70 ◽  
Ex Vivo ◽  
Granule Secretion ◽  
And Function

Molecular chaperones that support protein quality control, including heat shock protein 70 (Hsp70), participate in diverse aspects of cellular and physiological function. Recent studies have reported roles for specific chaperone activities in blood platelets in maintaining hemostasis; however, the functions of Hsp70 in platelet physiology remain uninvestigated. Here we characterize roles for Hsp70 activity in platelet activation and function. In vitro biochemical, microscopy, flow cytometry, and aggregometry assays of platelet function, as well as ex vivo analyses of platelet aggregate formation in whole blood under shear, were carried out under Hsp70-inhibited conditions. Inhibition of platelet Hsp70 blocked platelet aggregation and granule secretion in response to collagen-related peptide (CRP), which engages the immunoreceptor tyrosine-based activation motif-bearing collagen receptor glycoprotein VI (GPVI)-Fc receptor-γ chain complex. Hsp70 inhibition also reduced platelet integrin-αIIbβ3activation downstream of GPVI, as Hsp70-inhibited platelets showed reduced PAC-1 and fibrinogen binding. Ex vivo, pharmacological inhibition of Hsp70 in human whole blood prevented the formation of platelet aggregates on collagen under shear. Biochemical studies supported a role for Hsp70 in maintaining the assembly of the linker for activation of T cells signalosome, which couples GPVI-initiated signaling to integrin activation, secretion, and platelet function. Together, our results suggest that Hsp70 regulates platelet activation and function by supporting linker for activation of T cells-associated signaling events downstream of platelet GPVI engagement, suggesting a role for Hsp70 in the intracellular organization of signaling systems that mediate platelet secretion, “inside-out” activation of platelet integrin-αIIbβ3, platelet-platelet aggregation, and, ultimately, hemostatic plug and thrombus formation.

scholarly journals Role of inducible heat shock protein 70 in radiation-induced cell death

2001 ◽  
Vol 6 (3) ◽  
pp. 273 ◽  
Author(s):  
Su-Jae Lee ◽  
Sun-Ah Choi ◽  
Kang-Hyun Lee ◽  
Hee-Yong Chung ◽  
Tae-Hwan Kim ◽  
...  
Keyword(s):  
Cell Death ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Radiation Induced

scholarly journals Prevention of UVB Radiation-induced Epidermal Damage by Expression of Heat Shock Protein 70

2009 ◽  
Vol 285 (8) ◽  
pp. 5848-5858 ◽  
Author(s):  
Minoru Matsuda ◽  
Tatsuya Hoshino ◽  
Yasuhiro Yamashita ◽  
Ken-ichiro Tanaka ◽  
Daisuke Maji ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Uvb Radiation ◽  
Radiation Induced
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