Melatonin mediates protective effects on inflammatory response induced by interleukin-1 beta in human mesenchymal stem cells

2013 ◽  
Vol 55 (1) ◽  
pp. 14-25 ◽  
Author(s):  
Xiaozhen Liu ◽  
Yihong Gong ◽  
Ke Xiong ◽  
Yun Ye ◽  
Yuanyan Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Inflammatory Response ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells ◽  
Protective Effects ◽  
Interleukin 1 Beta

scholarly journals Baculovirus Transduction of Mesenchymal Stem Cells Triggers the Toll-Like Receptor 3 Pathway

2009 ◽  
Vol 83 (20) ◽  
pp. 10548-10556 ◽  
Author(s):  
Guan-Yu Chen ◽  
Hsiao-Chiao Shiah ◽  
Hung-Ju Su ◽  
Chi-Yuan Chen ◽  
Yung-Jen Chuang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Viral Vectors ◽  
Viral Vector ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells ◽  
Cytokine Expression ◽  
Toll Like Receptor ◽  
Safety Concerns ◽  
Surface Marker Expression

ABSTRACT Human mesenchymal stem cells (hMSCs) can be genetically modified with viral vectors and hold promise as a cell source for regenerative medicine, yet how hMSCs respond to viral vector transduction remains poorly understood, leaving the safety concerns unaddressed. Here, we explored the responses of hMSCs against an emerging DNA viral vector, baculovirus (BV), and discovered that BV transduction perturbed the transcription of 816 genes associated with five signaling pathways. Surprisingly, Toll-like receptor-3 (TLR3), a receptor that generally recognizes double-stranded RNA, was apparently upregulated by BV transduction, as confirmed by microarray, PCR array, flow cytometry, and confocal microscopy. Cytokine array data showed that BV transduction triggered robust secretion of interleukin-6 (IL-6) and IL-8 but not of other inflammatory cytokines and beta interferon (IFN-β). BV transduction activated the signaling molecules (e.g., Toll/interleukin-1 receptor domain-containing adaptor-inducing IFN-β, NF-κB, and IFN regulatory factor 3) downstream of TLR3, while silencing the TLR3 gene with small interfering RNA considerably abolished cytokine expression and promoted cell migration. These data demonstrate, for the first time, that a DNA viral vector can activate the TLR3 pathway in hMSCs and lead to a cytokine expression profile distinct from that in immune cells. These findings underscore the importance of evaluating whether the TLR3 signaling cascade plays roles in the immune response provoked by other DNA vectors (e.g., adenovirus). Nonetheless, BV transduction barely disturbed surface marker expression and induced only transient and mild cytokine responses, thereby easing the safety concerns of using BV for hMSCs engineering.

scholarly journals Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Elena Redondo-Castro ◽  
Catriona Cunningham ◽  
Jonjo Miller ◽  
Licia Martuscelli ◽  
Sarah Aoulad-Ali ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells ◽  
Anti Inflammatory

scholarly journals Neupogen and mesenchymal stem cells are the novel therapeutic agents in regeneration of induced endometrial fibrosis in experimental rats

2017 ◽  
Vol 37 (5) ◽  
Author(s):  
Dina Sabry ◽  
Abeer Mostafa ◽  
Samar Marzouk ◽  
Walaa Ibrahim ◽  
Hanan H.M. Ali ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Combined Therapy ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells ◽  
Tissue Growth ◽  
Collagen I ◽  
Albino Rats

Endometrial fibrosis is the presence of intrauterine adhesions (IUAs) after any uterine surgery or curettage and it results in infertility and recurrent pregnancy loss. We evaluated the role of human mesenchymal stem cells (hMSCs) as a therapeutic agent of endometrial fibrosis. We also compared the effect of MSCs with the effect of estrogen and neupogen either each alone or as a combined therapy with MSCs. This experimental study was performed on 84 albino rats which were divided into seven groups (n=12 rats/group) as follows, group1: normal control rats, group 2: induced fibrosis, group 3: induced fibrosis that received oral estrogen, group 4: induced fibrosis that received hMSCs, group 5: induced fibrosis that received hMSCs and estrogen, group 6: induced fibrosis that received neupogen, and group 7: induced fibrosis that received hMSCs and neupogen. The extent of fibrosis, vascularization, and inflammation were evaluated by; qRT-PCR for interleukin 1 (IL-1), interleukin 6 (IL-6), TNF, vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and RUNX; ELISA for connective tissue growth factor (CTGF); Western blotting for collagen-I; immunohistochemistry examination for VEGF and RUNX-2; and histopathological assessment. In therapeutic groups either by hMSCs alone or combined with estrogen or neupogen; fibrosis and inflammation (IL-1, IL-6, TNF, TGF-β, RUNX, CTGF, and collagen-I) were significantly decreased but vascularization (VEGF) was significantly increased (P<0.05) compared with induced fibrosis group. The most significant result was obtained in fibrosis that received combined therapy of hMSCs and neupogen (P=0.000). Stem cells and neupogen are a highly effective alternative regenerative agents in endometrial fibrosis.

Human mesenchymal stem cells promote survival and prevent intestinal damage in a mouse model of radiation injury

RSC Advances ◽  
2016 ◽  
Vol 6 (69) ◽  
pp. 65105-65111 ◽  
Author(s):  
Meng-Zheng Guo ◽  
Wei Gong ◽  
Hong-Wei Zhang ◽  
Yan Wang ◽  
Li-Qing Du ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mouse Model ◽  
Radiation Injury ◽  
Human Mesenchymal Stem Cells ◽  
Intestinal Stem Cell ◽  
Human Umbilical Cord ◽  
Intestinal Damage ◽  
Protective Effects

In the present study, we examined the protective effects of human umbilical cord mesenchymal stem cells (hMSCs) against intestinal stem cell (ISC) death and intestinal damage in a mouse model of radiation injury.

scholarly journals Changes in the secretome of tri-dimensional spheroid-cultured human mesenchymal stem cells in vitro by interleukin-1 priming

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Elena Redondo-Castro ◽  
Catriona J. Cunningham ◽  
Jonjo Miller ◽  
Helena Brown ◽  
Stuart M. Allan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells

Interleukin 1 β-induced SMAD2/3 linker modifications are TAK1 dependent and delay TGFβ signaling in primary human mesenchymal stem cells

2017 ◽  
Vol 40 ◽  
pp. 190-199 ◽  
Author(s):  
Guus G. van den Akker ◽  
Henk M. van Beuningen ◽  
Elly L. Vitters ◽  
Marije I. Koenders ◽  
Fons A. van de Loo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Interleukin 1 ◽  
Human Mesenchymal Stem Cells ◽  
Tgfβ Signaling

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
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