Basic fibroblast growth factor signalling regulates cancer stem cells in lung cancer A549 cells

2019 ◽  
Vol 71 (9) ◽  
pp. 1412-1420 ◽  
Author(s):  
Yousheng Meng ◽  
Xiaoyan Bai ◽  
Yuandong Huang ◽  
Lang He ◽  
Zhengwei Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Stem Cells ◽  
Growth Factor ◽  
Cancer Stem Cells ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
A549 Cells ◽  
Fibroblast Growth ◽  
Growth Factor Signalling

scholarly journals Inhibition activity of a disulfide-stabilized diabody against basic fibroblast growth factor in lung cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (12) ◽  
pp. 20187-20197 ◽  
Author(s):  
Yaxiong Cai ◽  
Shuange Yao ◽  
Jiangchuan Zhong ◽  
Jinxia Zhang ◽  
Haowu Jiang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Inhibition Activity

Morphological Differentiation of Astroglial Progenitor Cells from Egf-Responsive Neurospheres in Response to Fetal Calf Serum, Basic Fibroblast Growth Factor, and Retinol

1996 ◽  
Vol 5 (2) ◽  
pp. 179-189 ◽  
Author(s):  
Yung H. Chiang ◽  
Vincenzo Silani ◽  
Feng C. Zhou
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Progenitor Cells ◽  
Basic Fibroblast Growth Factor ◽  
Calf Serum ◽  
Basal Medium ◽  
Morphological Differentiation ◽  
Fibroblast Growth ◽  
Brain Areas

Procurement of multipotential neuroglial stem cells is possible with the addition of epidermal growth factor (EGF). Stem cells will differentiate into neurons and glia upon the removal of EGF from the culture medium. We have previously characterized the neuronal differentiation of stem cells derived from long-term cultured nonpassage neurospheres. In the current study, we (1) characterize the morphological differentiation of the astroglial progenitor cell from 3-mo-old neurospheres, (2) examine whether the astroglial progenitor cells from neurospheres of different brain areas exhibit different differentiation responses to the same exogenous signals, and (3) test the effects of basic fibroblast growth factor (bFGF) and retinol on differentiation. Cerebral cortex, striatum, and mesencephalon cells were obtained from Embryonic Day 14 (E-14) rat fetuses and were dissociated for the procurement of neurospheres in chemically defined medium supplemented with EGF. After 3 mo in culture, the neurospheres, derived from each of the three brain areas, were subcultured into three groups on chamber slides: (1) basal medium, (2) the basal medium plus 20 ng/mL bFGF, and (3) the basal medium plus 10 μM retinol. Phenotypic expression of astroglial cells was examined after 14 days subculture. Our findings indicate that the 3-mo-old cultured nonpassage neurospheres contained numerous multipotential stem cells that stained positive with nestin, and that environmental factors played an important role in influencing the differentiation of astroglial progenitor cells. As detected by glial fibrillary acid protein (GFAP), astroglial progenitor cells turned into protoplasmic astrocytes in the FCS-containing basal medium, fibrous astrocytes in the presence of bFGF, and spindle-shaped astrocytes in the presence of retinol. There were no noticeable differences in differentiation among astroglial progenitor cells of the various brain region-derived neurospheres in any of the three medium conditions. Peculiar varicosity-and growth cone-like structures on the long slender GFAP-positive processes suggest that neuroblasts and glioblast may share common morphologies, features, or common progenitor cells during initial differentiation in vitro.

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