Cytotoxic analysis and chemical characterization of fractions of the hydroalcoholic extract of the Euterpe oleracea Mart. seed in the MCF-7 cell line

2017 ◽  
Vol 69 (6) ◽  
pp. 714-721 ◽  
Author(s):  
Dayanne da S. Freitas ◽  
José A. Morgado-Díaz ◽  
Adriana S. Gehren ◽  
Flávia C. B. Vidal ◽  
Raquel Maria T. Fernandes ◽  
...  
Keyword(s):  
Cell Line ◽  
Chemical Characterization ◽  
Euterpe Oleracea ◽  
Hydroalcoholic Extract ◽  
Euterpe Oleracea Mart ◽  
Mcf 7

scholarly journals Antioxidant Capacity and Chemical Characterization of Açaí (Euterpe oleracea Mart.) Fruit Fractions

2016 ◽  
Vol 4 (5) ◽  
pp. 95-102 ◽  
Author(s):  
Daniela Souza Ferreira ◽  
Alex Linardi Gomes ◽  
Marta Gomes da Silva ◽  
Adriana Barreto Alves ◽  
Wellington Hortenci Dall Agnol ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Chemical Characterization ◽  
Euterpe Oleracea ◽  
Euterpe Oleracea Mart

Preparation and Characterization of the Nutritive Value of Flour Made from Acai (Euterpe oleracea, Mart.) Seeds

2014 ◽  
Vol 4 (3) ◽  
pp. 224-228
Author(s):  
Elaine Lima ◽  
Edna Santos ◽  
Robert Smith ◽  
Armando Sabaa-Srur
Keyword(s):  
Nutritive Value ◽  
Euterpe Oleracea ◽  
Euterpe Oleracea Mart

scholarly journals Real-Time Challenging of ERα Y537S Mutant Transcriptional Activity in Living Cells

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 54-64
Author(s):  
Manuela Cipolletti ◽  
Sara Pescatori ◽  
Filippo Acconcia
Keyword(s):  
Cell Line ◽  
Transcriptional Activity ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Patient Treatment ◽  
Estrogen Responsive Element ◽  
Specific Proteins ◽  
Responsive Element ◽  
Mcf 7

Metastatic estrogen receptor α (ERα)-expressing breast cancer (BC) occurs after prolonged patient treatment with endocrine therapy (ET) (e.g., aromatase inhibitors—AI; 4OH-tamoxifen—4OH-Tam). Often these metastatic BCs express a mutated ERα variant (e.g., Y537S), which is transcriptionally hyperactive, sustains uncontrolled proliferation, and renders tumor cells insensitive to ET drugs. Therefore, new molecules blocking hyperactive Y537S ERα mutation transcriptional activity are requested. Here we generated an MCF-7 cell line expressing the Y537S ERα mutation stably expressing an estrogen-responsive element (ERE) promoter, which activity can be monitored in living cells. Characterization of this cell line shows both hyperactive basal transcriptional activity with respect to normal MCF-7 cells, which stably express the same ERE-based promoter and a decreased effect of selective ER downregulators (SERDs) in reducing Y537S ERα mutant transcriptional activity with respect to wild type ERα transcriptional activity. Kinetic profiles of Y537S ERα mutant-based transcription produced by both drugs inducing receptor degradation and siRNA-mediated depletion of specific proteins (e.g., FOXA1 and caveolin1) reveals biphasic dynamics of the inhibition of the receptor-regulated transcriptional effects. Overall, we report a new model where to study the behavior of the Y537S ERα mutant that can be used for the identification of new targets and pathways regulating the Y537S ERα transcriptional activity.

Brominated phenols: characterization of estrogen-like activity in the human breast cancer cell-line MCF-7

2002 ◽  
Vol 129 (1-2) ◽  
pp. 55-63 ◽  
Author(s):  
Christel M Olsen ◽  
Elise T.M Meussen-Elholm ◽  
Jørn A Holme ◽  
Jan K Hongslo
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Human Breast Cancer ◽  
Cancer Cell Line ◽  
Human Breast Cancer Cell ◽  
Human Breast ◽  
Mcf 7

scholarly journals Effect of Hydroalcoholic Extract of Ephedramajor, Memordia charantia, and Resveratrol on Cytotoxicity and Caspase-3 Genes Expression Level in MCF-7 Breast Cancer Cell Line

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Seyed Kazem Sabbagh ◽  
Ehsan Ghodrati ◽  
Alireza Hajibeiki ◽  
Mahta Mazaheri ◽  
Mohammad Reza Sarafraz Ardakani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Medicinal Plants ◽  
Cell Viability ◽  
Cell Line ◽  
Plant Extracts ◽  
Caspase 3 ◽  
Cell Toxicity ◽  
Hydroalcoholic Extract ◽  
Expression Levels ◽  
Mcf 7

Background: To increase the therapeutic effect of drugs to combat diseases, combination therapy with current chemical drugs and new medicines derived from medicinal plants is necessary. Objectives: The present work aimed to investigate the effect of hydroalcoholic extract of two medicinal plants, Ephedra major and Momordi cacharantia (Carla), and resveratrol drug on cell viability and expression levels of caspase-3 gene in MCF-7 cell line. Methods: In this experimental study, the hydroalcoholic extraction of tested plants was done with a Soxhlet extractor. The MTT assay and real-time PCR were used to determine cell toxicity and caspase-3 gene expression levels, respectively. Results: The highest and lowest cytotoxic effects of plant extracts and resveratrol were observed at concentrations of 500 and 150 µg/mL, respectively. The highest level of the caspase-3 gene expression was observed after 72 h of incubation by different concentrations of plant extracts and resveratrol. Conclusions: It can be concluded that both plant extracts could influence cell viability in MCF-7 cells via the increase of cell toxicity and expression of caspase3 gene. Thus, these species could be used in the pharmaceutical industry.

Spray drying of extract from Euterpe oleracea Mart.: Optimization of process and characterization of the açaí powder

2019 ◽  
Vol 42 (8) ◽  
Author(s):  
Maria da C. da Costa Valente ◽  
Rafael A. Nascimento ◽  
Elza B. Santana ◽  
Nielson F. da Paixão Ribeiro ◽  
Cristiane M. L. Costa ◽  
...  
Keyword(s):  
Spray Drying ◽  
Euterpe Oleracea ◽  
Euterpe Oleracea Mart

scholarly journals Characterization of a vitamin D3-resistant MCF-7 cell line.

Endocrinology ◽  
1996 ◽  
Vol 137 (2) ◽  
pp. 400-409 ◽  
Author(s):  
C J Narvaez ◽  
K Vanweelden ◽  
I Byrne ◽  
J Welsh
Keyword(s):  
Cell Line ◽  
Vitamin D3 ◽  
Mcf 7
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