The immunomodulatory effect of propolis on receptors expression, cytokine production and fungicidal activity of human monocytes

2014 ◽  
Vol 66 (10) ◽  
pp. 1497-1504 ◽  
Author(s):  
Michelle Cristiane Búfalo ◽  
Ana Paula Bordon-Graciani ◽  
Bruno José Conti ◽  
Marjorie de Assis Golim ◽  
José Maurício Sforcin
Keyword(s):  
Fungicidal Activity ◽  
Cytokine Production ◽  
Immunomodulatory Effect ◽  
Human Monocytes

scholarly journals Cinnamic Acid Is Partially Involved in Propolis Immunomodulatory Action on Human Monocytes

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Bruno José Conti ◽  
Michelle Cristiane Búfalo ◽  
Marjorie de Assis Golim ◽  
Vassya Bankova ◽  
José Maurício Sforcin
Keyword(s):  
Cinnamic Acid ◽  
Fungicidal Activity ◽  
Cytokine Production ◽  
Biological Properties ◽  
Human Monocytes ◽  
Antigen Receptors ◽  
Hla Dr ◽  
Complex Chemical Composition ◽  
High Concentrations ◽  
Well Data

Propolis is a beehive product used in traditional medicine due to its biological properties. It shows a complex chemical composition including phenolics, such as cinnamic acid (Ci). The mechanisms of action of propolis have been the subject of research recently; however, the involvement of Ci on propolis activity was not investigated on immune cells. Ci effects were evaluated on human monocytes, assessing the expression of Toll-like receptors (TLRs), HLA-DR, and CD80. Cytokine production (TNF-αand IL-10) and the fungicidal activity of monocytes were evaluated as well. Data showed that Ci downregulated TLR-2, HLA-DR, and CD80 and upregulated TLR-4 expression by human monocytes. High concentrations of Ci inhibited both TNF-αand IL-10 production, whereas the same concentrations induced a higher fungicidal activity againstCandida albicans. TNF-αand IL-10 production was decreased by blocking TLR-4, while the fungicidal activity of monocytes was not affected by blocking TLRs. These results suggest that Ci modulated antigen receptors, cytokine production, and the fungicidal activity of human monocytes depending on concentration, and TLR-4 may be involved in its mechanism of action. Ci seemed to be partially involved in propolis activities.

scholarly journals Antifungal antibiotics modulate the pro-inflammatory cytokine production and phagocytic activity of human monocytes in an in vitro sepsis model

Life Sciences ◽  
2015 ◽  
Vol 141 ◽  
pp. 128-136 ◽  
Author(s):  
Stefan Muenster ◽  
Christian Bode ◽  
Britta Diedrich ◽  
Sebastian Jahnert ◽  
Christina Weisheit ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Inflammatory Cytokine ◽  
Cytokine Production ◽  
Human Monocytes ◽  
Inflammatory Cytokine Production ◽  
Sepsis Model ◽  
Antifungal Antibiotics

scholarly journals Bovine glycomacropeptide induces cytokine production in human monocytes through the stimulation of the MAPK and the NF-κB signal transduction pathways

2009 ◽  
Vol 157 (7) ◽  
pp. 1232-1240 ◽  
Author(s):  
Pilar Requena ◽  
Abdelali Daddaoua ◽  
Emilia Guadix ◽  
Antonio Zarzuelo ◽  
María Dolores Suárez ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cytokine Production ◽  
Signal Transduction Pathways ◽  
Human Monocytes ◽  
Stimulation Of

scholarly journals Regulation of Cellular Metabolism and Cytokines by the Medicinal Herb Feverfew in the Human Monocytic THP-1 Cells

2009 ◽  
Vol 6 (1) ◽  
pp. 91-98 ◽  
Author(s):  
Chin-Fu Chen ◽  
Chun-Huai Cheng
Keyword(s):  
Cytokine Production ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cellular Metabolism ◽  
Mechanisms Of Action ◽  
Human Monocytes ◽  
Cellular Targets ◽  
Tnf Α ◽  
Folk Remedy ◽  
Mediate Metabolism

The herb feverfew is a folk remedy for various symptoms including inflammation. Inflammation has recently been implicated in the genesis of many diseases including cancers, atherosclerosis and rheumatoid arthritis. The mechanisms of action of feverfew in the human body are largely unknown. To determine the cellular targets of feverfew extracts, we have utilized oligo microarrays to study the gene expression profiles elicited by feverfew extracts in human monocytic THP-1 cells. We have identified 400 genes that are consistently regulated by feverfew extracts. Most of the genes are involved in cellular metabolism. However, the genes undergoing the highest degree of change by feverfew treatment are involved in other pathways including chemokine function, water homeostasis and heme-mediated signaling. Our results also suggest that feverfew extracts effectively reduce Lipopolysaccharides (LPS)-mediated TNF-α and CCL2 (MCP-1) releases by THP-1 cells. We hypothesize that feverfew components mediate metabolism, cell migration and cytokine production in human monocytes/macrophages.

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