A Rapid and Efficient Method for In Vitro Screening of Taro for Leaf Blight Disease Caused by Phytophthora colocasiae

2016 ◽  
Vol 164 (7-8) ◽  
pp. 520-527
Author(s):  
Vishnu Sukumari Nath ◽  
Shyni Basheer ◽  
Muthulekshmi Lajapathy Jeeva ◽  
Vinayaka Mahabaleswar Hegde ◽  
Asha Devi ◽  
...  
Keyword(s):  
Efficient Method ◽  
Leaf Blight ◽  
In Vitro Screening ◽  
Phytophthora Colocasiae ◽  
Blight Disease

Thymol and its Derivatives for Management of Phytopathogenic fungi of maize

2021 ◽  
Vol 18 ◽  
Author(s):  
Jyoti Gaba ◽  
Sunita Sharma ◽  
Harleen Kaur ◽  
Pardeep Kaur
Keyword(s):  
Pathogenic Fungi ◽  
Bioactive Compound ◽  
Mannich Bases ◽  
Phytopathogenic Fungi ◽  
Leaf Blight ◽  
Significant Control ◽  
Thymol Derivatives ◽  
Potential Alternative ◽  
Blight Disease

Background: Thymol is a bioactive compound having many pharmacological activities. Objective: The present study was carried out to evaluate the fungi toxic effects of thymol and derivatives against phytopathogenic fungi of maize. Method: Thymol was derivatized to get formylated thymol, Mannich bases, and imine derivatives. All the synthesized thymol derivatives were characterized by their physical and spectral properties. Synthesized thymol derivatives were screened for their in vitro antifungal effects using poisoned food technique against three maize pathogenic fungi namely Fusarium moniliforme, Rhizoctonia solani and Dreschlera maydis. Results: Thymol and formylated thymol showed promising results for control of D. maydis with ED50 values less than standard carbendazim and comparable to standard mancozeb. These two compounds were further evaluated for control of D. maydis causative maydis leaf blight disease on maize plants grown in the field during the Kharif season (June to October) 2018. Conclusion: Thymol exhibited significant control of maydis leaf blight disease of maize and emerged as a potential alternative to synthetic fungicides used in cereal crops.

scholarly journals Prevalence and Severity of Leaf Blight Disease of Chrysanthemum in Southern regions of Karnataka and in-vitro Fungicidal Effect on Alternaria alternata

2015 ◽  
Vol 9 (3) ◽  
pp. 38-45 ◽  
Author(s):  
G. Shamala ◽  
G. R. Janardhana
Keyword(s):  
Alternaria Alternata ◽  
Disease Incidence ◽  
Life Sciences ◽  
Leaf Blight ◽  
Cut Flowers ◽  
In Vitro Evaluation ◽  
Karnataka State ◽  
Commercially Important ◽  
Blight Disease

Chrysanthemum is one of the commercially important flower crop with great potential both as loose and cut flowers in Karnataka state. In the present study, prevalence and incidence of leaf blight disease was studied in six major growing districts of South Karnataka during 2012 - 2013. The pathogen associated with leaf blight disease was isolated and identified as Alternaria alternata. The study revealed that, the disease was distributed in Southern districts of Karnataka wherever Chrysanthemum is grown. The varieties Chandini and Karnel were more susceptible to blight showing highest disease incidence (100%) and severity (100%) recorded from Mandya and Mysore regions. The lowest disease incidence, severity and PDI of 18.40%, 38.68% and 24.89% were recorded on Rajawhite variety in Bangalore region. During 2013, the highest disease incidence, severity and PDI with 100%, 98.47%, and 45.71% were recorded in Mysore district on Chandini variety. In vitro evaluation of four fungicides on Alternaria alternata revealed that Carbendazim+Mancozeb was most effective with 95.65% inhibition followed by Carbendazim, Mancozeb and Metalaxyl+Mancozeb with 68.26%, 91.30% and 91.30% inhibition of the mycelium at 2.0% concentration.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12465   International Journal of Life Sciences 9 (3): 2015; 38-45

scholarly journals In Vitro Screening of Antibacterial Agents for Suppression of Fire Blight Disease in Korea

2018 ◽  
Vol 24 (1) ◽  
pp. 41-51
Author(s):  
Min Su Lee ◽  
Ingyeong Lee ◽  
Sam Kyu Kim ◽  
Chang-Sik Oh ◽  
Duck Hwan Park
Keyword(s):  
Antibacterial Agents ◽  
Fire Blight ◽  
In Vitro Screening ◽  
Blight Disease

Morphological, pathological and molecular characterization of Phytophthora colocasiae responsible for taro leaf blight disease in India

2014 ◽  
Vol 43 (1) ◽  
pp. 21-35 ◽  
Author(s):  
Vishnu Sukumari Nath ◽  
Vinayaka Mahabaleswar Hegde ◽  
Muthulekshmi Lajapathy Jeeva ◽  
Raj Shekar Misra ◽  
Syamala Swayamvaran Veena ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Leaf Blight ◽  
Phytophthora Colocasiae ◽  
Blight Disease ◽  
Taro Leaf Blight

In vitro screening for Botrytis leaf blight resistance in Lilium species

2018 ◽  
Vol 239 ◽  
pp. 133-140
Author(s):  
Ji-Young Jang ◽  
Saminathan Subburaj ◽  
Geung-Joo Lee ◽  
Hyun-Soon Kim
Keyword(s):  
Leaf Blight ◽  
Blight Resistance ◽  
In Vitro Screening ◽  
Lilium Species

scholarly journals Detached-Leaf Bioassay for Evaluating Taro Resistance to Phytophthora colocasiae

Plant Disease ◽  
2008 ◽  
Vol 92 (1) ◽  
pp. 126-131 ◽  
Author(s):  
F. E. Brooks
Keyword(s):  
Pacific Islands ◽  
Cropping Systems ◽  
American Samoa ◽  
Leaf Blight ◽  
Limiting Factor ◽  
Detached Leaf ◽  
Phytophthora Colocasiae ◽  
Highly Correlated ◽  
Blight Disease ◽  
Taro Leaf Blight

Taro leaf blight disease, caused by Phytophthora colocasiae, is a major limiting factor in taro production worldwide. P. colocasiae is an aerial pathogen similar to P. infestans, causal agent of potato late blight disease, but occurs in warmer climates. In the year-round subsistence cropping systems of the Pacific Islands, resistant cultivars are essential. Breeding lines from Southeast Asia and Oceania were tested in American Samoa for resistance to taro leaf blight using a detached-leaf bioassay and field trials. Mean lesion diameters from bioassays were highly correlated with field estimates of the number of healthy leaves per plant and yield (corm weight). However, the bioassay did not adequately assess infection efficiency. Additional experiments revealed that attached leaves had smaller lesion diameters than detached leaves incubated in closed containers, but both were very highly correlated. Taro resistance increased with plant age and the second-oldest leaf was more resistant than the third-oldest leaf. The bioassay was a fast, space-saving, effective method of screening taro lines for post-penetration resistance to P. colocasiae. It also provided an easily standardized method of evaluating host–pathogen interactions under controlled conditions.

scholarly journals Severity of Phytophthora leaf blight disease and susceptibility of two local varieties of Colocasia to Phytophthora colocasiae Raciborski in Nsukka zone of South Eastern Nigeria

Bio-Research ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 1286-1296
Author(s):  
Faustina Njideka Ugwuja ◽  
Chiemeka Nwakaego Onaebi ◽  
Nneka Virginia Chiejina ◽  
Kelvin Ikechukwu Ugwuoke
Keyword(s):  
Disease Severity ◽  
Leaf Surface ◽  
Average Diameter ◽  
Colocasia Esculenta ◽  
Leaf Blight ◽  
Test Results ◽  
Local Varieties ◽  
Potted Plants ◽  
Phytophthora Colocasiae ◽  
Blight Disease

Leaf-blight disease of Colocasia caused by Phytophthora colocasiae Raciborski has been a serious impediment to cocoyam production in Nigeria. Disease severity and susceptibility of the two most cultivated local varieties “Ugwuta” (Colocasia esculenta var. antiquorum) and “Nkashi Colocasia esculenta var. esculenta) were investigated. Disease severity was visually estimated as the percentage leaf surface affected by blight, lesion or lesion-related chlorosis for each leaf of a plant using a seven-point scale of 0, 5, 10, 25, 50, 75 and 100% in three locations: Ede-Oballa, Nsukka Urban and Obukpa. Susceptibility was assessed on 2 months old potted plants of each variety inoculated with a 7-day old culture of P. colocasiae. Diameters of lesions on inoculated leaves were recorded from the 3rd - 8th day after inoculation.  Data on severity were subjected to ANOVA and susceptibility of the varieties was compared with t-test. Results revealed significant LSD=4.96 (0.05) and varying degrees of leaf blight severity among varieties and locations. Variety antiquorum had significantly higher severities of 42.08, 46.40 and 47.42% at Ede-Oballa, Nsukka Urban and Obukpa respectively, compared to 34.85, 36.55 and 28.19% recorded by var. esculenta at these locations, respectively. Similarly, var. antiquorum had greater lesion diameter ranging from 0.65±0.07 cm - 3.70±0.14 cm and average diameter of 2.4±0.16cm compared to var. esculenta which had 0.41±0.14cm - 3.12±0.19 cm and average diameter of 1.80±0.16. This research has shown that varieties and locations affect the severity and susceptibility of Phytophthora leaf blight disease. This could be a guide to farmers having known that var. esculenta is less severe to Phytophthora leaf blight disease.

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