scholarly journals Caveolin‐1 promotes cancer progression via inhibiting ferroptosis in head and neck squamous cell carcinoma

2021 ◽  
Author(s):  
Tingwei Lu ◽  
Zhen Zhang ◽  
Xinhua Pan ◽  
Jianjun Zhang ◽  
Xu Wang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Caveolin 1

scholarly journals Intrinsic Oncogenic Function of Intracellular Connexin26 Protein in Head and Neck Squamous Cell Carcinoma Cells

2018 ◽  
Vol 19 (7) ◽  
pp. 2134 ◽  
Author(s):  
Nobuko Iikawa ◽  
Yohei Yamamoto ◽  
Yohei Kawasaki ◽  
Aki Nishijima-Matsunobu ◽  
Maya Suzuki ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Squamous Cell Carcinoma ◽  
Cell Membrane ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Gap Junction ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Junction Protein

It has long been known that the gap junction is down-regulated in many tumours. One of the downregulation mechanisms is the translocation of connexin, a gap junction protein, from cell membrane into cytoplasm, nucleus, or Golgi apparatus. Interestingly, as tumours progress and reinforce their malignant phenotype, the amount of aberrantly-localised connexin increases in different malignant tumours including oesophageal squamous cell carcinoma, thus suggesting that such an aberrantly-localised connexin should be oncogenic, although gap junctional connexins are often tumour-suppressive. To define the dual roles of connexin in head and neck squamous cell carcinoma (HNSCC), we introduced the wild-type connexin26 (wtCx26) or the mutant Cx26 (icCx26) gene, the product of which carries the amino acid sequence AKKFF, an endoplasmic reticulum-Golgi retention signal, at the C-terminus and is not sorted to cell membrane, into the human FaDu hypopharyngeal cancer cell line that had severely impaired the expression of connexin during carcinogenesis. wtCx26 protein was trafficked to the cell membrane and formed gap junction, which successfully exerted cell-cell communication. On the other hand, the icCx26 protein was co-localised with a Golgi marker, as revealed by immunofluorescence, and thus was retained on the way to the cell membrane. While the forced expression of wtCx26 suppressed both cell proliferation in vitro and tumorigenicity in mice in vivo, icCx26 significantly enhanced both cell proliferation and tumorigenicity compared with the mock control clones, indicating that an excessive accumulation of connexin protein in intracellular domains should be involved in cancer progression and that restoration of proper subcellular sorting of connexin might be a therapeutic strategy to control HNSCC.

scholarly journals Tumor microenvironment characterization and Immune infiltration in head and neck squamous cell carcinoma

2019 ◽  
Author(s):  
zenghong wu ◽  
xun niu ◽  
Xi-Yue Xiao ◽  
xiong chen
Keyword(s):  
Immune Response ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Survival Function ◽  
Neck Squamous Cell Carcinoma ◽  
Immune Infiltration ◽  
Significant Difference

Abstract Objection: Head and neck squamous cell carcinoma (HNSCC), a basic malignant tumor of the head and neck distinct. As a famous heterogeneous disease, the jobs of immune cells inside the tumor-related as yet missing for HNSCC, particularly in current immunotherapy. Method: We explored the TME, TMB and evaluate the 22 TIICs subsets of immune response based on GEO and TCGA database of HNSCC to explore its relationship with atomic subpopulation, survival, function and expression difference and reveal potential targets and biomarkers for immunotherapy. Results: Observing the download of GSE6631 database contained 22 HNSCC samples and 22 normal samples and TCGA database contained 111 HNSCC and 12 normal tissues. The results suggested that the expression of macrophages M0 and T cells CD4 memory resting was significant difference and may plays an important role in regulate cancer progression (P<0.05). The result of tumor mutational burden revealed that the most common somatic mutations variant classification was missense mutation, the most common DNA sequence polymorphism type was SNP, the most common single nucleotide variants (SNV) class was C>T, the variants per sample median was 78 in HNSCC patients. Top 10 mutated genes that related to TMB was TP53, TTN, FAT1, MUC16, CDKN2A, CSMD3, SYNE1, LRP1B, NOTCH1 and PIK3CA. We portrayed the immune scene in detail, uncovering the awesome immune infiltration styles of various subtypes in HNSCC. Conclusion: The intricate connection between TIIC, TMB and genomic alterations was additionally set up. Our paintings advance the information of immune response and offers significant assets for research to enhance immunotherapy.

scholarly journals Tumor microenvironment characterization and Immune infiltration in head and neck squamous cell carcinoma

2020 ◽  
Author(s):  
Zenghong Wu ◽  
Xun Niu ◽  
Xi-Yue Xiao ◽  
Xiong Chen
Keyword(s):  
Immune Response ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Survival Function ◽  
Neck Squamous Cell Carcinoma ◽  
Immune Infiltration ◽  
Significant Difference

Abstract Background : Head and neck squamous cell carcinoma (HNSCC), a basic malignant tumor of the head and neck distinct. As a famous heterogeneous disease, the jobs of immune cells inside the tumor-related as yet missing for HNSCC, particularly in current immunotherapy. Method: We explored the TME, TMB and evaluate the 22 TIICs subsets of immune response based on GEO and TCGA database of HNSCC to explore its relationship with atomic subpopulation, survival, function and expression difference and reveal potential targets and biomarkers for immunotherapy. Results: Observing the download of GSE6631 database contained 22 HNSCC samples and 22 normal samples and TCGA database contained 111 HNSCC and 12 normal tissues. The results suggested that the expression of macrophages M0 and T cells CD4 memory resting was significant difference and may plays an important role in regulate cancer progression ( P <0.05). The result of tumor mutational burden revealed that the most common somatic mutations variant classification was missense mutation, the most common DNA sequence polymorphism type was SNP, the most common single nucleotide variants (SNV) class was C>T, the variants per sample median was 78 in HNSCC patients. Top 10 mutated genes that related to TMB was TP53, TTN, FAT1, MUC16, CDKN2A, CSMD3, SYNE1, LRP1B, NOTCH1 and PIK3CA. We portrayed the immune scene in detail, uncovering the awesome immune infiltration styles of various subtypes in HNSCC. Conclusion: The intricate connection between TIIC, TMB and genomic alterations was additionally set up. Our paintings advance the information of immune response and offers significant assets for research to enhance immunotherapy.

scholarly journals Restoration of caveolin-1 expression suppresses growth and metastasis of head and neck squamous cell carcinoma

2008 ◽  
Vol 99 (10) ◽  
pp. 1684-1694 ◽  
Author(s):  
H Zhang ◽  
L Su ◽  
S Müller ◽  
M Tighiouart ◽  
Z Xu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Caveolin 1

scholarly journals CD200 Induces Epithelial-to-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma via β-Catenin-Mediated Nuclear Translocation

Cancers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1583 ◽  
Author(s):  
Seung-Phil Shin ◽  
A-RA Goh ◽  
Hyeon-Gu Kang ◽  
Seok-Jun Kim ◽  
Jong-Kwang Kim ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Nuclear Translocation ◽  
Neck Squamous Cell Carcinoma ◽  
Solid Cancer ◽  
Mesenchymal Transition

The membrane glycoprotein CD200 binds to its receptor CD200R1 and induces tolerance, mainly in cells of the myeloid lineage; however, information regarding its role in solid tumors is limited. Here, we investigated whether CD200 expression, which is enriched mainly in high-grade head and neck squamous cell carcinoma (HNSCC), correlates with cancer progression, particularly the epithelial-to-mesenchymal transition (EMT). The forced overexpression of CD200 in the HNSCC cell line, UMSCC84, not only increased the expression of EMT-related genes, but also enhanced invasiveness. The cleaved cytoplasmic domain of CD200 interacted with β-catenin in the cytosol, was translocated to the nucleus, and eventually enhanced EMT-related gene expression. CD200 increased the invasiveness of mouse tonsillar epithelium immortalized with E6, E7, and Ras (MEER), a model of tonsillar squamous cell carcinoma. siRNA inhibition of CD200 or extracellular domain of CD200R1 down-regulated the expression of EMT-related genes and decreased invasiveness. Consistently, compared to CD200-null MEER tumors, subcutaneous CD200-expressing MEER tumors showed significantly increased metastatic migration into draining lymph nodes. Our study demonstrates a novel and unique role of CD200 in inducing EMT, suggesting the potential therapeutic target for blocking solid cancer progression.

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