scholarly journals Different patterns of expression of cell cycle control and local invasion-related proteins in oral squamous cell carcinoma affecting young patients

2017 ◽  
Vol 47 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Marisol Miranda Galvis ◽  
Alan Roger Santos-Silva ◽  
Juscelino Freitas Jardim ◽  
Felipe Paiva Fonseca ◽  
Marcio A. Lopes ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cell Cycle Control ◽  
Young Patients ◽  
Local Invasion ◽  
Related Proteins

scholarly journals α-Mangostin Induces Apoptosis and Cell Cycle Arrest in Oral Squamous Cell Carcinoma Cell

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Hyun-Ho Kwak ◽  
In-Ryoung Kim ◽  
Hye-Jin Kim ◽  
Bong-Soo Park ◽  
Su-Bin Yu
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Cycle Arrest ◽  
Squamous Cell ◽  
G1 Phase ◽  
Cycle Arrest ◽  
Related Proteins

Mangosteen has long been used as a traditional medicine and is known to have antibacterial, antioxidant, and anticancer effects. Although the effects ofα-mangostin, a natural compound extracted from the pericarp of mangosteen, have been investigated in many studies, there is limited data on the effects of the compound in human oral squamous cell carcinoma (OSCC). In this study,α-mangostin was assessed as a potential anticancer agent against human OSCC cells.α-Mangostin inhibited cell proliferation and induced cell death in OSCC cells in a dose- and time-dependent manner with little to no effect on normal human PDLF cells.α-Mangostin treatment clearly showed apoptotic evidences such as nuclear fragmentation and accumulation of annexin V and PI-positive cells on OSCC cells.α-Mangostin treatment also caused the collapse of mitochondrial membrane potential and the translocation of cytochrome c from the mitochondria into the cytosol. The expressions of the mitochondria-related proteins were activated byα-mangostin. Treatment withα-mangostin also induced G1 phase arrest and downregulated cell cycle-related proteins (CDK/cyclin). Hence,α-mangostin specifically induces cell death and inhibits proliferation in OSCC cells via the intrinsic apoptosis pathway and cell cycle arrest at the G1 phase, suggesting thatα-mangostin may be an effective agent for the treatment of OSCC.

scholarly journals Expression of cell cycle proteins according to HPV status in oral squamous cell carcinoma affecting young patients: a pilot study

2018 ◽  
Vol 125 (4) ◽  
pp. 317-325 ◽  
Author(s):  
Marisol Miranda Galvis ◽  
Juscelino Freitas Jardim ◽  
Estela Kaminagakura ◽  
Alan Roger Santos-Silva ◽  
Felipe Paiva Fonseca ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Pilot Study ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Young Patients ◽  
Cell Cycle Proteins ◽  
Hpv Status

scholarly journals Mutation status of p53 gene in oral squamous cell carcinoma

2009 ◽  
Vol 56 (4) ◽  
pp. 171-175 ◽  
Author(s):  
Branka Popovic ◽  
Biljana Jekic ◽  
Drago Jelovac ◽  
Ivana Novakovic
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Point Mutation ◽  
Squamous Cell ◽  
Cell Cycle Control ◽  
P53 Gene ◽  
Suppressor Gene ◽  
Protein Product

Introduction. p53 gene is the most common tumor suppressor gene involved in pathogenesis oral squamous cell carcinoma (OSCC). Protein product of p53 gene contributes to cell cycle control and apoptosis. p53 gene mutations may lead to uncontrolled cell growth. The aim of this study was to determine the incidence of mutation in DNA-binding domain of p53 gene. Materials and Methods. In the 60 specimens, the presence of point mutation in exons 5, 6, 7 and 8 was detected using PCR-SSCP method. To confirm the presence of p53 mutation found by SSCP method, five samples were analyzed by sequencing of exon 5. Results. Point mutation affecting exons 5, 6, 7 and 8 were found in 60% of analyzed samples. A higher incidence of mutation was detected in exon 7 and 8 (60%), than in exon 5 and 6. Sequencing of exon 5, confirmed the presence of mutations revealed by SSCP method. Study of associations showed an increase of p53 mutations in poor differentiated and carcinoma of higher clinical stages. Conclusion. p53 gene is one of major factor in control of cell cycle and has important role in pathogenesis of oral squamous cell carcinoma.

scholarly journals Cyclin D1 Expression and Its Correlation with Histopathological Differentiation in Oral Squamous Cell Carcinoma

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Swati Saawarn ◽  
Madhusudan Astekar ◽  
Nisheeth Saawarn ◽  
Nidhi Dhakar ◽  
Shitalkumar Gomateshwar Sagari
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
Cell Cycle Control ◽  
Clinical Staging ◽  
Formalin Fixed Paraffin Embedded ◽  
Well Differentiated

Background. Cyclin D1 regulates the G1 to S transition of cell cycle. Its deregulation or overexpression may lead to disturbance in the normal cell cycle control and tumour formation. Overexpression of cyclin D1 has been reported in various tumors of diverse histogenesis. This case control retrospective study was carried out to study the immunohistochemical reactivity and expression of cyclin D1 and its association with site, clinical staging, and histopathological differentiation of oral squamous cell carcinoma (OSCC).Methods. Forty formalin-fixed paraffin-embedded tissue blocks of biopsy specimens of oral squamous cell carcinoma were immunohistochemically evaluated for expression of cyclin D1.Results. Cyclin D1 expression was seen in 45% cases of OSCC. It did not correlate with site and clinical staging. Highest expression was seen in well-differentiated, followed by moderately differentiated, and poorly differentiated squamous cell carcinomas, with a statistically significant correlation.Conclusion. Cyclin D1 expression significantly increases with increase in differentiation.

Cyclosporine A Suppresses the Malignant Progression of Oral Squamous Cell Carcinoma in vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 248-255 ◽  
Author(s):  
Ling Gao ◽  
Jianwei Dong ◽  
Nanyang Zhang ◽  
Zhanxian Le ◽  
Wenhao Ren ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cyclosporine A ◽  
Migration And Invasion ◽  
Signal Molecules ◽  
Cox 2

Background:The Oral Squamous Cell Carcinoma (OSCC) is one of the most frequent cancer types. Failure of treatment of OSCC is potentially lethal because of local recurrence, regional lymph node metastasis, and distant metastasis. Chemotherapy plays a vital role through suppression of tumorigenesis. Cyclosporine A (CsA), an immunosuppressant drug, has been efficiently used in allograft organ transplant recipients to prevent rejection, and also has been used in a subset of patients with autoimmunity related disorders. The present study aims to investigate novel and effective chemotherapeutic drugs to overcome drug-resistance in the treatment of OSCC.Methods:Cells were incubated in the standard way. Cell viability was assayed using the MTT assay. Cell proliferation was determined using colony formation assay. The cell cycle assay was performed using flow cytometry. Apoptosis was assessed using fluorescence-activated cell sorting after stained by the Annexin V-fluorescein isothiocyanate (FITC). Cell migration and invasion were analyzed using wound healing assay and tranwell. The effect of COX-2, c-Myc, MMP-9, MMP-2, and NFATc1 protein expression was determined using Western blot analysis while NFATc1 mRNA expression was determined by RT-PCR.Results:In vitro studies indicated that CsA inhibited partial OSCC growth by inducing cell cycle arrest, apoptosis, and the migration and invasion of OSCC cells. We also demonstrated that CsA could inhibit the expression of NFATc1 and its downstream genes COX-2, c-Myc, MMP-9, and MMP-2 in OSCC cells. Furthermore, we analyzed the expression of NFATc1 in head and neck cancer through the Oncomine database. The data was consistent with the experimental findings.Conclusion:The present study initially demonstrated that CsA could inhibit the progression of OSCC cells and can mediate the signal molecules of NFATc1 signaling pathway, which has strong relationship with cancer development. That explains us CsA has potential to explore the possibilities as a novel chemotherapeutic drug for the treatment of OSCC.

Association between cell cycle gene transcription and tumor size in oral squamous cell carcinoma

Tumor Biology ◽  
2015 ◽  
Vol 36 (12) ◽  
pp. 9717-9722 ◽  
Author(s):  
Marina Gonçalves Diniz ◽  
Jeane de Fatima Correia Silva ◽  
Fabricio Tinôco Alvim de Souza ◽  
Núbia Braga Pereira ◽  
Carolina Cavaliéri Gomes ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Gene Transcription ◽  
Squamous Cell ◽  
Tumor Size ◽  
Cell Cycle Gene
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