A role for cancer-associated fibroblasts in inducing the epithelial-to-mesenchymal transition in human tongue squamous cell carcinoma

2014 ◽  
Vol 43 (8) ◽  
pp. 585-592 ◽  
Author(s):  
Bin Zhou ◽  
Wei-Liang Chen ◽  
You-Yuan Wang ◽  
Zhao-Yu Lin ◽  
Da-Ming Zhang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Tongue Squamous Cell Carcinoma ◽  
Cancer Associated Fibroblasts ◽  
Mesenchymal Transition ◽  
Human Tongue

scholarly journals AKT3 Is a Novel Regulator of Cancer-Associated Fibroblasts in Head and Neck Squamous Cell Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1233
Author(s):  
Hideyuki Takahashi ◽  
Susumu Rokudai ◽  
Reika Kawabata-Iwakawa ◽  
Koichi Sakakura ◽  
Tetsunari Oyama ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Mrna Expression ◽  
Squamous Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Neck Squamous Cell Carcinoma ◽  
The Cancer Genome Atlas ◽  
Cancer Associated Fibroblasts ◽  
Mesenchymal Transition

Cancer-associated fibroblasts (CAFs) play vital roles in tumor progression by promoting epithelial-to-mesenchymal transition, angiogenesis, and immunosuppression. In the present study, we sought to identify the key regulators of the pro-tumoral functions of CAFs in head and neck squamous cell carcinoma (HNSCC). mRNA expression data obtained from The Cancer Genome Atlas revealed that CAF-specific mRNA expression correlated with genes that relate to an immunosuppressive microenvironment in a HNSCC cohort. RNA sequencing of CAFs and normal fibroblasts isolated from HNSCC specimens identified 1127 differentially expressed genes (DEGs) and several upregulated pathways in CAFs. Among the 1127 DEGs, we identified 13 immune function-related genes and focused on AKT3 as a potential regulator of CAFs. The targeted depletion of AKT3 in CAFs revealed that AKT3 promotes their myofibroblastic phenotype. AKT3-transduced CAFs exhibited downregulated the expression of immunosuppressive cytokine genes, impairing T-cell suppression and pro-tumoral macrophage induction. The immunohistochemistry of 72 HNSCC patients showed that AKT3 expression in CAFs positively correlated with tumor infiltration by CAFs, tumor-associated macrophages, dendritic cells, and T cells. Moreover, AKT3 expression in CAFs was an independent prognostic factor for overall survival. In conclusion, AKT3 is a potential target for cancer therapy that inhibits the pro-tumoral function of CAFs and reverses CAF-mediated immunosuppression.

scholarly journals Markers of Epithelial to Mesenchymal Transition in Association with Survival in Head and Neck Squamous Cell Carcinoma (HNSCC)

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94273 ◽  
Author(s):  
Eirini Pectasides ◽  
Theodoros Rampias ◽  
Clarence Sasaki ◽  
Christos Perisanidis ◽  
Vassilis Kouloulias ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Epithelial To Mesenchymal Transition ◽  
Neck Squamous Cell Carcinoma ◽  
Mesenchymal Transition
Sign in / Sign up

Export Citation Format

Share Document