Reduced immune responses to purified protein derivative and Candida albicans in oral lichen planus

2013 ◽  
Vol 42 (9) ◽  
pp. 691-697 ◽  
Author(s):  
Charlotte Simark-Mattsson ◽  
Christina Eklund
Keyword(s):  
Candida Albicans ◽  
Immune Responses ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Purified Protein Derivative ◽  
Oral Lichen

scholarly journals A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

2019 ◽  
Author(s):  
Hong He ◽  
Mengyun Zhang ◽  
Xinyu Xia ◽  
Haiping Yang ◽  
Qiao Peng
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Random Amplified Polymorphic Dna ◽  
Type I ◽  
Similarity Coefficients ◽  
Professional Recognition ◽  
Normal Individuals ◽  
Polymerase Chain ◽  
Oral Lichen

Abstract Objectives：This study aimed to investigate the prevalence and genotypic profiles of Candida albicans from patients with oral lichen planus (OLP). Materials andMethods：Genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reaction were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results：The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients S AB were 100%, and inter-groups similarity coefficients S AB were less than 30%. Conclusions：The genotypic evidence of C. albicans in OLP might inferred an endogenous infection and some etiologic sense contributing to professional recognition on the development and progression of OLP for more suitable diagnose and treatment.

scholarly journals A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

2020 ◽  
Author(s):  
Hong He ◽  
Xinyu Xia ◽  
Haiping Yang ◽  
Qiao Peng ◽  
Jiaoer Zheng
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Random Amplified Polymorphic Dna ◽  
Type I ◽  
Similarity Coefficients ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Normal Individuals ◽  
Oral Lichen

Abstract Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.

scholarly journals Candida albicans induces TLR2/MyD88/NF-κB signaling and inflammation in oral lichen planus-derived keratinocytes

2018 ◽  
Vol 12 (09) ◽  
pp. 780-786
Author(s):  
Jianwei Liu ◽  
Fanghui Geng ◽  
Hongying Sun ◽  
Xiaxia Wang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Signaling Pathway ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Mucosal Disease ◽  
Mrna And Protein Expression ◽  
Key Events ◽  
The Impact ◽  
Oral Lichen ◽  
Tlr2 Mrna

Introduction: The risk of oral lichen planus (OLP), a chronic inflammatory oral mucosal disease, becoming malignant increases by 21-fold in patients with fungal infection. This study examined the impact of Candida albicans exposure on Toll-like receptor (TLR) signaling in primary keratinocyte cultures obtained from OLP patients. Methodology: Following co-culture of primary OLP keratinocyte cultures with C. albicans for 24 hours, inflammatory cytokine concentrations were determined by ELISA. TLR2, MyD88, and NF-κBp65 mRNA and protein expression were assessed using quantitative RT-PCR and Western blot analyses, respectively. Keratinocyte apoptosis was also determined by flow cytometry. Results: IL-10, IL-8, IL-2, and TNF-ɑ levels were significantly higher following co-culture with C. albicans (all p ≤ 0.034). MyD88, NF-κB p65, and TLR2 mRNA (all p < 0.001) and protein (all p ≤ 0.004) expression levels were significantly higher in OLP keratinocytes following C. albicans exposure. Finally, the apoptosis rates of OLP keratinocytes were 21.2%, 29.4%, and 25.4% for the control cells and 3.9%, 5.6%, and 4.4% for those exposed to C. albicans, suggesting that co-culture with C. albicans inhibits the apoptosis of OLP keratinocytes. Conclusions: C. albicans activates the TLR2/MyD88/NF-κB signaling pathway in OLP keratinocytes, resulting in increased cytokine expression and decreased keratinocyte apoptosis. Two key events in the pathogenesis of OLP and its progression to malignancy, namely increased inflammation and decreased apoptosis, were induced by exposure to C. albicans. Thus, targeting this signaling pathway may represent a novel therapeutic strategy to prevent OLP malignant transformation.

Genotypic profiles and virulence attributes of Candida albicans isolates from patients with oral lichen planus

Apmis ◽  
2008 ◽  
Vol 116 (4) ◽  
pp. 284-91 ◽  
Author(s):  
XIN ZENG ◽  
CHUNRONG XIONG ◽  
ZHI WANG ◽  
LU JIANG ◽  
XIAOHUI HOU ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Oral Lichen

scholarly journals Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173017 ◽  
Author(s):  
Masaki Yamauchi ◽  
Masafumi Moriyama ◽  
Jun-Nosuke Hayashida ◽  
Takashi Maehara ◽  
Noriko Ishiguro ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Immune Responses ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Thymic Stromal Lymphopoietin ◽  
Myeloid Dendritic Cells ◽  
Oral Lichen

scholarly journals Detection and identification of non-Candida albicans species in human oral lichen planus

2010 ◽  
Vol 55 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Mika Masaki ◽  
Takuichi Sato ◽  
Yumiko Sugawara ◽  
Takashi Sasano ◽  
Nobuhiro Takahashi
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Detection And Identification ◽  
Oral Lichen

scholarly journals Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hong He ◽  
Ying Wang ◽  
Yan Fan ◽  
Congcong Li ◽  
Jianxin Han
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
In Vitro Study ◽  
Essential Genes ◽  
Reverse Transcription Pcr ◽  
Significant Difference ◽  
Yeast Phase ◽  
Oral Lichen

Abstract Background Hypha essential genes (HEGs) of Candida Albicans have been emerging into scholar’s attention, little known about their functions in oral lichen planus (OLP) with an uncovered etiology. This research aimed to observe necessary genes in biphasic C. albicans from OLP and study their relevance in pathogenesis, so as to evaluate possible roles of morphologic switching in etiology of OLP. Methods Samples were collected from OLP lesions of patients, mycelia were cultured and total RNA was extracted then subjected to reverse transcription-PCR and real-time PCR. Results HWP1 and HGC1 were significantly expressed in hyphae phase and weakly detected in yeast phase, while there was no significant difference of EFG1, ALS3, and ECE1 between in yeast and mycelia. Conclusion HGC1 and HWP1 were confirmed to be hypha essential genes, with HGC1 for hypha morphogenesis and HWP1 for adhesion invasion in pathogenesis of C. albicans in OLP. ALS3, ECE1 and EFG1 played minor roles in hyphae maintenance and adhesion for hyphae. These might be deemed as hints for the etiology of OLP and indicate HGC1 and HWP1 to be a priority of potential drug target.

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