Intrauterine transplantation of autologous menstrual blood stem cells increases endometrial thickness and pregnancy potential in patients with refractory intrauterine adhesion

2020 ◽  
Vol 46 (11) ◽  
pp. 2347-2355 ◽  
Author(s):  
Hailan Ma ◽  
Mengting Liu ◽  
Yufeng Li ◽  
Wei Wang ◽  
Keqin Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endometrial Thickness ◽  
Menstrual Blood ◽  
Intrauterine Adhesion ◽  
Menstrual Blood Stem Cells ◽  
Blood Stem Cells

scholarly journals Intrauterine transplantation of autologous menstrual blood stem cells in the treatment of refractory intrauterine adhesion: a prospective cohort study

2019 ◽  
Author(s):  
Hailan Ma ◽  
Mengting Liu ◽  
Dongcheng Wu ◽  
Yufeng Li ◽  
Wei Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endometrial Thickness ◽  
Transvaginal Ultrasound ◽  
Hormone Replacement ◽  
Post Treatment ◽  
Menstrual Blood ◽  
Intrauterine Adhesion ◽  
Menstrual Blood Stem Cells ◽  
Blood Stem Cells ◽  
Pre Treatment

Abstract Background: The loss of endometrial stem cells has been associated with intrauterine adhesion caused by miscarriage, repeated improper intrauterine operation or infection. Cell-based therapies are promising in regenerative medicine. However, the effects of menstrual blood stem cells (MenSCs) therapy on impaired endometrium need to be explored and validated. This study evaluates the effects of intrauterine transplantation of MenSCs on endometrial thickness and pregnancy outcomes in patients with refractory intrauterine adhesion. Methods: This study included a group of infertile women (n=12, age 22-40 years), with refractory intrauterine adhesion (Grade III – IV). Autologous MenSCs isolated from the women’ menstrual blood were expanded in vitro and transplanted into their uteruses, followed by hormone replacement therapy. Transvaginal ultrasound examination was performed to assess the endometrial thickness. Transabdominal ultrasound was conducted to detect pregnancy outcome. Results: Autologous MenSCs were successfully isolated and expanded from menstrual blood and transplanted into the uterus of each patient. A significant improvement of the endometrial thickness was observed (from 3.9±0.9 mm pre-treatment to 7.5±0.6 mm post-treatment (P<0.001)). No adverse reaction was observed. The duration of menstruation was increased (from 2.4±0.7 days pre-treatment to 5.3±0.6 days post-treatment (P<0.001)). Five out of 12 patients achieved clinical pregnancy. Conclusions: Intrauterine transplantation of autologous MenSCs results in regeneration of endometrium, a prolongation of menstrual duration and an increase rate of pregnancy in patients with refractory intrauterine adhesion.

scholarly journals Menstrual Blood-Derived Endometrial Stem Cells’ Impact for the Treatment Perspective of Female Infertility

2021 ◽  
Vol 22 (13) ◽  
pp. 6774
Author(s):  
Giedrė Skliutė ◽  
Raminta Baušytė ◽  
Veronika Borutinskaitė ◽  
Giedrė Valiulienė ◽  
Algirdas Kaupinis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell Differentiation ◽  
Embryonic Cell ◽  
Menstrual Blood ◽  
Endometrial Stem Cells ◽  
Expression Of Genes ◽  
Menstrual Blood Stem Cells ◽  
Marrow Mesenchymal Stem Cells ◽  
Blood Stem Cells ◽  
Inflammatory Processes

When looking for the causes and treatments of infertility, much attention is paid to one of the reproductive tissues—the endometrium. Therefore, endometrial stem cells are an attractive target for infertility studies in women of unexplained origin. Menstrual blood stem cells (MenSCs) are morphologically and functionally similar to cells derived directly from the endometrium; with dual expression of mesenchymal and embryonic cell markers, they proliferate and regenerate better than bone marrow mesenchymal stem cells. In addition, menstrual blood stem cells are extracted in a non-invasive and painless manner. In our study, we analyzed the characteristics and the potential for decidualization of menstrual blood stem cells isolated from healthy volunteers and women diagnosed with infertility. We demonstrated that MenSCs express CD44, CD166, CD16, CD15, BMSC, CD56, CD13 and HLA-ABC surface markers, have proliferative properties, and after induction of menstrual stem cell differentiation into epithelial direction, expression of genes related to decidualization (PRL, ESR, IGFBP and FOXO1) and angiogenesis (HIF1, VEGFR2 and VEGFR3) increased. Additionally, the p53, p21, H3K27me3 and HyperAcH4 proteins’ expression increased during MenSCs decidualization, they secrete proteins that are involved in the regulation of the actin cytoskeleton, estrogen and relaxin signaling pathways and the management of inflammatory processes. Our findings reveal the potential use of MenSCs for the treatment of reproductive disorders.

Evaluation of menstrual blood stem cells seeded in biocompatible Bombyx mori silk fibroin scaffold for cardiac tissue engineering

2014 ◽  
Vol 29 (2) ◽  
pp. 199-208 ◽  
Author(s):  
Maryam Rahimi ◽  
Homa Mohseni-Kouchesfehani ◽  
Amir-Hassan Zarnani ◽  
Sahba Mobini ◽  
Shohreh Nikoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Menstrual Blood ◽  
Silk Fibroin Scaffold ◽  
Menstrual Blood Stem Cells ◽  
Blood Stem Cells ◽  
Bombyx Mori Silk

Comparative restoration of acute liver failure by menstrual blood stem cells compared with bone marrow stem cells in mice model

Cytotherapy ◽  
2017 ◽  
Vol 19 (12) ◽  
pp. 1474-1490 ◽  
Author(s):  
Mina Fathi-Kazerooni ◽  
Gholamreza Tavoosidana ◽  
Masoud Taghizadeh-Jahed ◽  
Sayeh Khanjani ◽  
Hananeh Golshahi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Bone Marrow Stem Cells ◽  
Menstrual Blood ◽  
Mice Model ◽  
Menstrual Blood Stem Cells ◽  
Blood Stem Cells

scholarly journals Therapeutic potential of menstrual blood stem cells in treating acute liver failure

2019 ◽  
Vol 25 (41) ◽  
pp. 6190-6204 ◽  
Author(s):  
Pan-Pan Cen ◽  
Lin-Xiao Fan ◽  
Jie Wang ◽  
Jia-Jia Chen ◽  
Lan-Juan Li
Keyword(s):  
Stem Cells ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Therapeutic Potential ◽  
Menstrual Blood ◽  
Menstrual Blood Stem Cells ◽  
Blood Stem Cells

scholarly journals The Potential of Menstrual Blood-Derived Mesenchymal Stem Cells for Cartilage Repair and Regeneration: Novel Aspects

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
I. Uzieliene ◽  
G. Urbonaite ◽  
Z. Tachtamisevaite ◽  
A. Mobasheri ◽  
E. Bernotiene
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cartilage Regeneration ◽  
Menstrual Blood ◽  
Easy Access ◽  
Differentiation Potential ◽  
Surgical Interventions ◽  
Menstrual Blood Stem Cells ◽  
Blood Stem Cells

Menstrual blood is a unique body fluid that contains mesenchymal stem cells (MSCs). These cells have attracted a great deal of attention due to their exceptional advantages including easy access and frequently accessible sample source and no need for complex ethical and surgical interventions, as compared to other tissues. Menstrual blood-derived MSCs possess all the major stem cell properties and even have a greater proliferation and differentiation potential as compared to bone marrow-derived MSCs, making them a perspective tool in a further clinical practice. Although the potential of menstrual blood stem cells to differentiate into a large variety of tissue cells has been studied in many studies, their chondrogenic properties have not been extensively explored and investigated. Articular cartilage is susceptible to traumas and degenerative diseases, such as osteoarthritis, and has poor self-regeneration capacity and therefore requires more effective therapeutic technique. MSCs seem promising candidates for cartilage regeneration; however, no clinically effective stem cell-based repair method has yet emerged. This chapter focuses on studies in the field of menstrual blood-derived MSCs and their chondrogenic differentiation potential and suitability for application in cartilage regeneration. Although a very limited number of studies have been made in this field thus far, these cells might emerge as an efficient and easily accessible source of multipotent cells for cartilage engineering and cell-based chondroprotective therapy.

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