Application of an in vitro reconstructed human skin on cosmetics in skin irritation tests

2020 ◽  
Author(s):  
Xiao Ma ◽  
Feifei Wang ◽  
Bo Wang
Keyword(s):  
Human Skin ◽  
Skin Irritation ◽  
Reconstructed Human Skin

scholarly journals Evaluation of Inflammation by Cytokine Production Following Combined Exposure to Ultraviolet and Radiofrequency Radiation of Mobile Phones on 3D Reconstructed Human Skin In Vitro

2020 ◽  
Vol 17 (12) ◽  
pp. 4401
Author(s):  
Zsófia Szilágyi ◽  
Zsuzsanna Németh ◽  
József Bakos ◽  
Péter Pál Necz ◽  
Anna Sáfár ◽  
...  
Keyword(s):  
Human Skin ◽  
Uv Radiation ◽  
Enzyme Concentration ◽  
Protective Effects ◽  
Mobile System ◽  
Wireless Devices ◽  
Rf Exposure ◽  
Solar Uv ◽  
Reconstructed Human Skin

The absorption of exposure to radiofrequency (RF) emitted by wireless devices leads to a high specific absorption rate in the skin. Ultraviolet (UV) radiation can induce several damages to the skin. The aim of this study was to examine whether combined, consecutive exposure to solar UV radiation and 1950 MHz RF exposure of third generation (3G) mobile system have any effect on inflammation processes in the skin. Under in vitro experiments, the inflammation process was examined by cytokines (IL-1α, IL-6, and IL-8) and MMP-1 enzyme secretion on 3D full thickness human skin model. The RF exposure was applied before or after UV irradiation, in order to study either the possible cooperative or protective effects of exposure to RF and UV. We did not find changes in cytokines due to exposure to RF alone. The RF exposure did not enhance the effects of UV radiation. There was a statistically not-significant decrease in cytokines when the skin tissues were pre-exposed to RF before being exposed to 4 standard erythemal dose (SED) UV compared to UV exposure alone. We found that RF exposure reduced the previously UV-treated MMP-1 enzyme concentration. This study might support the evaluation of the effects on the skin exposed to microwave radiation of 5G mobile technology.

In Vitro Skin Irritation Testing of Petroleum-Based Compounds in Reconstituted Human Skin Models

1994 ◽  
Vol 13 (1) ◽  
pp. 23-37 ◽  
Author(s):  
Francis J. Koschier ◽  
Randy N. Roth ◽  
Thomas J. Stephens ◽  
E. Tiffany Spence ◽  
Mary Ann Duke
Keyword(s):  
Human Skin ◽  
Skin Irritation ◽  
Human Skin Models

Use of Human Skin Equivalent Apligraf for in Vitro Assessment of Cumulative Skin Irritation Potential of Topical Products

2000 ◽  
Vol 164 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Jesús Medina ◽  
Anne de Brugerolle de Fraissinette ◽  
Salah-Dine Chibout ◽  
Maryelle Kolopp ◽  
Rahel Kammermann ◽  
...  
Keyword(s):  
Human Skin ◽  
Skin Irritation ◽  
Skin Equivalent ◽  
In Vitro Assessment ◽  
Human Skin Equivalent

scholarly journals Effect of 2-acetylaminofluorene and its genotoxic metabolites on DNA adduct formation and DNA damage in 3D reconstructed human skin tissue models

Mutagenesis ◽  
2019 ◽  
Author(s):  
Thomas R Downs ◽  
Volker M Arlt ◽  
Brenda C Barnett ◽  
Ryan Posgai ◽  
Stefan Pfuhler
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Skin ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Strong Increase ◽  
Multiple Exposures ◽  
Multiple Treatments ◽  
Reconstructed Human Skin

Abstract In vitro genotoxicity assays utilising human skin models are becoming important tools for the safety assessment of chemicals whose primary exposure is via the dermal route. In order to explore metabolic competency and inducibility of CYP450 activating enzymes, 3D reconstructed human skin tissues were topically treated with 2-acetylaminofluorene (2-AAF) and its genotoxic metabolites, N-hydroxy-2-acetylaminofluorene (N-OH-2-AAF) and N-hydroxy-2-aminofluorene (N-OH-2-AF), which primarily cause DNA damage by forming DNA adducts. 2-AAF did not increase DNA damage measured in the reconstructed skin micronucleus (RSMN) assay when administered in multiple applications at 24 h intervals but was detected in the skin comet assay in the presence of the DNA polymerase inhibitor aphidicolin (APC). Similarly, no increase was found with N-OH-2-AAF in the RSMN assay after multiple treatments whereas a single 3 h exposure to N-OH-2-AAF caused a large dose-related increase in the skin comet assay. A significant increase in the RSMN assay was only obtained with the highly reactive N-OH-2-AF metabolite after multiple treatments over 72 h, whereas N-OH-2-AF caused a strong increase after a single 3 h exposure in the skin comet assay. In support of these results, DNA adduct formation, measured by the 32P-postlabelling assay, was examined. Adduct levels after 2-AAF treatment for 3 h were minimal but increased >10-fold after multiple exposures over 48 h, suggesting that enzyme(s) that metabolise 2-AAF are induced in the skin models. As expected, a single 3 h exposure to N-OH-2-AAF and N-OH-2-AF resulted in adduct levels that were at least 10-fold greater than those after multiple exposures to 2-AAF despite ~100-fold lower tested concentrations. Our results demonstrate that DNA damage caused by 2-AAF metabolites is more efficiently detected in the skin comet assay than the RSMN assay and after multiple exposures and enzyme induction, 2-AAF-induced DNA damage can be detected in the APC-modified comet assay.

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