scholarly journals Multiple steps characterise ventricular layer attrition to form the ependymal cell lining of the adult mouse spinal cord central canal

2019 ◽  
Vol 236 (2) ◽  
pp. 334-350 ◽  
Author(s):  
Marco A. Cañizares ◽  
Aida Rodrigo Albors ◽  
Gail Singer ◽  
Nicolle Suttie ◽  
Metka Gorkic ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Adult Mouse ◽  
Ependymal Cell ◽  
Central Canal ◽  
Mouse Spinal Cord ◽  
Cell Lining

scholarly journals Evidence for PKD2L1-positive neurons present distant from the central canal in the ventromedial spinal cord and Medulla of the adult mouse

2021 ◽  
Author(s):  
Nina JURCIC ◽  
Caroline MICHELLE ◽  
Jerome TROUSLARD ◽  
Nicolas WANAVERBECQ ◽  
Anne KASTNER
Keyword(s):  
Spinal Cord ◽  
Stem Cell Niche ◽  
Adult Mouse ◽  
Cell Layer ◽  
Ependymal Cell ◽  
Central Canal ◽  
Sensory Receptor ◽  
Neuronal Phenotype ◽  
Adult Mice ◽  
Cell Niche

Neurons in contact with the cerebrospinal fluid (CSF) are found around the medullo-spinal central canal (CC) in adult mice. These neurons (CSF-cNs), located within or below the ependymal cell layer known as the stem cell niche, present a characteristic morphology with a dendrite projecting to the CC and ending with a protrusion. They are GABAergic, characterized by an immature neuronal phenotype and selectively express PKD2L1, a channel member of the TRP channel superfamily with properties of sensory receptor. Using immunohistological techniques in mice, we characterize a new population of PKD2L1 positive cells that is observed around embryonic day 16 (E16), is present distant from the CC in a zone enriched with astrocytes and ependymal fibers of the ventro-medial spinal cord and medulla. With development, their number appears stable although smaller than that of CSF-cNs and they progressively become more distant from the CC with the reorganization of the CC region. These neurons share both functional and phenotypical properties with CSF-cNs, but they appear subdivided in two groups. One, present along the midline, has a bipolar morphology and extend a long dendrite along ependymal fibers and towards the CC. The second group, localized in more ventro-lateral regions, has a multipolar morphology and no apparent projection to the CC Altogether, we describe a novel population of PKD2L1+ neurons distant from the CC but with properties similar to CSF-cNs that might serve to sense modification in the composition of either CSF or interstitial liquid, a function that will need to be confirmed.

scholarly journals Single-cell transcriptomic analysis of the adult mouse spinal cord reveals molecular diversity of autonomic and skeletal motor neurons

2021 ◽  
Vol 24 (4) ◽  
pp. 572-583 ◽  
Author(s):  
Jacob A. Blum ◽  
Sandy Klemm ◽  
Jennifer L. Shadrach ◽  
Kevin A. Guttenplan ◽  
Lisa Nakayama ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Single Cell ◽  
Motor Neurons ◽  
Molecular Diversity ◽  
Adult Mouse ◽  
Transcriptomic Analysis ◽  
Mouse Spinal Cord

Repair of a myelin lesion by Schwann cells transplanted in the adult mouse spinal cord

1992 ◽  
Vol 40 (2-3) ◽  
pp. 235-242 ◽  
Author(s):  
A. Baron-Van Evercooren ◽  
A. Gansmuller ◽  
E. Duhamel ◽  
F. Pascal ◽  
M. Gumpel
Keyword(s):  
Spinal Cord ◽  
Schwann Cells ◽  
Adult Mouse ◽  
Mouse Spinal Cord ◽  
Myelin Lesion

Rapid isolation and culture of primary microglia from adult mouse spinal cord

2009 ◽  
Vol 183 (2) ◽  
pp. 223-237 ◽  
Author(s):  
Ping K. Yip ◽  
Timothy K.Y. Kaan ◽  
Daniel Fenesan ◽  
Marzia Malcangio
Keyword(s):  
Spinal Cord ◽  
Adult Mouse ◽  
Primary Microglia ◽  
Mouse Spinal Cord ◽  
Rapid Isolation

scholarly journals Identification of genome-wide targets of Olig2 in the adult mouse spinal cord using ChIP-Seq

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0186091 ◽  
Author(s):  
Andrew J. Darr ◽  
Matt C. Danzi ◽  
Lee Brady ◽  
Dorothea Emig-Agius ◽  
Amber Hackett ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Adult Mouse ◽  
Mouse Spinal Cord ◽  
Genome Wide

scholarly journals Intraspinal injection of adeno-associated viruses into the adult mouse spinal cord

2021 ◽  
Vol 2 (3) ◽  
pp. 100786
Author(s):  
Shrivas Chaterji ◽  
Arnab Barik ◽  
Anupama Sathyamurthy
Keyword(s):  
Spinal Cord ◽  
Adult Mouse ◽  
Mouse Spinal Cord ◽  
Intraspinal Injection

scholarly journals Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord

2021 ◽  
Author(s):  
Felix Fiederling ◽  
Luke A. Hammond ◽  
David Ng ◽  
Carol Mason ◽  
Jane Dodd
Keyword(s):  
Spinal Cord ◽  
Sample Preparation ◽  
High Throughput ◽  
Adult Mouse ◽  
Spatial Information ◽  
Spinal Neurons ◽  
Mouse Spinal Cord ◽  
Single Block ◽  
And Function

Spinal neurons are highly heterogeneous in location, transcriptional identity and function. To understand their contributions to sensorimotor circuits, it is essential to map the positions of identified subsets of neurons in relation to others throughout the spinal cord (SC), but we lack tools for whole SC sample preparation, imaging and in toto analysis. To overcome this problem, we have (1) designed scaffolds (SpineRacks) that facilitate efficient and ordered cryo-sectioning of the entire SC in a single block, (2) constructed a 3D reference atlas of adult mouse SC and (3) developed software (SpinalJ) to register images of sections and for standardized analysis of cells and projections in atlas space. We have verified mapping accuracies for known neurons and demonstrated the usefulness of this platform to reveal unknown neuronal distributions. Together, these tools provide high-throughput analyses of whole mouse SC and enable direct comparison of 3D spatial information between animals and studies.

scholarly journals Rho-Associated Kinase II (ROCKII) Limits Axonal Growth after Trauma within the Adult Mouse Spinal Cord

2009 ◽  
Vol 29 (48) ◽  
pp. 15266-15276 ◽  
Author(s):  
P. Duffy ◽  
A. Schmandke ◽  
A. Schmandke ◽  
J. Sigworth ◽  
S. Narumiya ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Adult Mouse ◽  
Axonal Growth ◽  
Mouse Spinal Cord

scholarly journals Exercise Training after Spinal Cord Injury Selectively Alters Synaptic Properties in Neurons in Adult Mouse Spinal Cord

2013 ◽  
Vol 30 (10) ◽  
pp. 891-896 ◽  
Author(s):  
Jamie R. Flynn ◽  
Lynda R. Dunn ◽  
Mary P. Galea ◽  
Robin Callister ◽  
Robert J. Callister ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Exercise Training ◽  
Adult Mouse ◽  
Mouse Spinal Cord ◽  
Cord Injury
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