scholarly journals The pisiform growth plate is lost in humans and supports a role for Hox in growth plate formation

2014 ◽  
Vol 225 (5) ◽  
pp. 527-538 ◽  
Author(s):  
Kelsey M. Kjosness ◽  
Jasmine E. Hines ◽  
C. Owen Lovejoy ◽  
Philip L. Reno
Keyword(s):  
Growth Plate ◽  
Plate Formation

scholarly journals Comparative development of mammalian and alligator metapodial growth plate formation

2006 ◽  
Vol 295 (1) ◽  
pp. 415
Author(s):  
Philip L. Reno ◽  
C. Owen Lovejoy ◽  
Ruth M. Elsey ◽  
Walter E. Horton
Keyword(s):  
Growth Plate ◽  
Comparative Development ◽  
Plate Formation

scholarly journals SOXC Transcription Factors Induce Cartilage Growth Plate Formation in Mouse Embryos by Promoting Noncanonical WNT Signaling

2015 ◽  
Vol 30 (9) ◽  
pp. 1560-1571 ◽  
Author(s):  
Kenji Kato ◽  
Pallavi Bhattaram ◽  
Alfredo Penzo‐Méndez ◽  
Abhilash Gadi ◽  
Véronique Lefebvre
Keyword(s):  
Transcription Factors ◽  
Wnt Signaling ◽  
Growth Plate ◽  
Mouse Embryos ◽  
Cartilage Growth ◽  
Plate Formation ◽  
Cartilage Growth Plate

scholarly journals Mice Deficient in NF-κB p50 and p52 or RANK Have Defective Growth Plate Formation and Post-natal Dwarfism

Bone Research ◽  
2013 ◽  
Vol 1 (4) ◽  
pp. 336-345 ◽  
Author(s):  
Lianping Xing ◽  
Di Chen ◽  
Brendan F. Boyce
Keyword(s):  
Growth Plate ◽  
Plate Formation

scholarly journals Quick and Abnormal Ossification Results in No Growth Plate Formation and Skeletal Dysplasia in Postnatal Mice Induced by Conditional Knockout of Indian Hedgehog

2020 ◽  
Author(s):  
Min Zhang ◽  
Jian Sun ◽  
Xiaochun Wei ◽  
Tao Yuan ◽  
Chunfang Wang ◽  
...  
Keyword(s):  
Growth Plate ◽  
Skeletal Dysplasia ◽  
Conditional Knockout ◽  
Indian Hedgehog ◽  
Micro Ct ◽  
Rt Pcr ◽  
Limb Deformity ◽  
X Ray ◽  
Parathyroid Hormone Related Protein ◽  
Plate Formation

Abstract Objective: To observe the dynamic effect of conditional knockout of Indian hedgehog (Ihh) on ossification of postnatal growth plate, skeletal abnormalities and the related signaling factors in mice.Methods: P0 Col2a1-Cre ERT2; Ihhfl/fl mice were randomly divided into tamoxifen (TM) injection to delete Ihh and oil injection as control. Real-time PCR (RT-PCR) was used to detect Ihh knockout rate. X-ray and micro-CT were used to evaluate gross and knee joint morphology of 8-week-old mice. The hind knee joints were harvested at P2-P14 after the animals were euthanized to observe the dynamic evolution of the growth plate. Safranin-O and Von Kossa staining were performed to assess growth plate development and the mineralization of bone respectively. The costal cartilage cells of mice were cultured in vitro on postnatal day 6. Changes in the expression of parathyroid hormone-related protein (PTHrP) and bone morphogenetic protein 6 (BMP-6) were detected by immunohistochemistry and RT-PCR.Results: RT-PCR results confirmed that the knockout rate of Ihh was 76.83%. X-ray and micro-CT scans showed that the short-limb deformity of the experimental group was associated with abnormal development of the epiphysis. Saffron O staining showed a disorder of cell arrange in the growth-plate area of Ihhd/d mice; Von Kossa staining showed an early and premature growth-plate ossification that prevents the growth plate formation in Ihhd/d mice starting at P8. Immunohistochemistry and RT-PCR showed significantly decreased PTHrP expression (P<0.05) and significantly increased BMP-6 expression (P<0.05). Conclusion: Postnatal conditional knockout of Ihh inhibited the expression of PTHrP and promotes the expression of BMP-6, which leads to early and abnormal ossification of the growth plate, no growth plate formation, and skeletal dysplasia in mice.

Effects of Hypophysectomy on the Occurrence and Calcifiability of Matrix Vesicles in the Epiphyseal Growth Plate of Immature Rats

1980 ◽  
Vol 38 ◽  
pp. 578-579
Author(s):  
S. I. Coleman ◽  
W. J. Dougherty
Keyword(s):  
Growth Plate ◽  
Matrix Vesicles ◽  
Epiphyseal Growth Plate ◽  
Hard Tissue ◽  
Hormonal Factors ◽  
Post Surgery ◽  
Mineral Deposition ◽  
Thin Sectioning ◽  
Integral Role ◽  
Cellular Secretion

In the cellular secretion theory of mineral deposition, extracellular matrix vesicles are believed to play an integral role in hard tissue mineralization (1). Membrane limited matrix vesicles arise from the plasma membrane of epiphyseal chondrocytes and tooth odontoblasts by a budding process (2, 3). Nutritional and hormonal factors have been postulated to play essential roles in mineral deposition and apparently have a direct effect on matrix vesicles of calcifying cartilage as concluded by Anderson and Sajdera (4). Immature (75-85 gm) Long-Evans hooded rats were hypophysectomized by the parapharyngeal approach and maintained fourteen (14) days post-surgery. At this time, the animals were anesthetized and perfusion fixed in cacodylate buffered 2.5% glutaraldehyde. The proximal tibias were quickly dissected out and split sagittally. One half was used for light microscopy (LM) and the other for electron microscopy (EM). The halves used for EM were cut into blocks approximately 1×3 mm. The tissue blocks were prepared for ultra-thin sectioning and transmission EM. The tissue was oriented so as to section through the epiphyseal growth plate from the zone of proliferating cartilage on down through the hypertrophic zone and into the initial trabecular bone. Sections were studied stained (double heavy metal) and unstained.

MICROCHEMICAL ANALYSIS OF HUMAN TIBIAL GROWTH CARTILAGE IN VARIOUS FORMS OF DWARFISM

1972 ◽  
Vol 69 (4) ◽  
pp. 659-688 ◽  
Author(s):  
V. Stanescu ◽  
R. Stanescu ◽  
J. A. Szirmai
Keyword(s):  
Growth Plate ◽  
Chondroitin Sulphate ◽  
Epiphyseal Plate ◽  
Molecular Characteristics ◽  
Growth Disorders ◽  
Normal Children ◽  
Collagen Concentration ◽  
Vitamin D Resistant Rickets ◽  
Pooled Samples ◽  
Tibial Epiphyseal

ABSTRACT Microchemical determinations of glycosaminoglycans and collagen were preformed in isolated histological zones from sections of tibial epiphyseal plate biopsies obtained from children with growth disorders (pituitary dwarfism, congenital myxoedema, Turner's syndrome, Noonan's syndrome, mucopolysaccharidosis type VI, vitamin D resistant rickets and achondroplasia). Alternate sections were used for histochemical localization of glycosaminoglycans and proteins. The values were compared with those found in comparable zones of the growth plate from normal children of the same age. The chondroitin sulphate concentration (% of defatted dry wt.) in the normal epiphyseal plate increased from the resting zone towards the proliferating/hypertrophic zone; collagen exhibited a reverse trend. In some of the pathological biopsies the concentration of chondroitin sulphate was slightly decreased whereas that of collagen was slightly increased. A marked increase in the collagen concentration was found in achondroplasia. The solubility profiles of the cetylpyridinium complexes of the chondroitin sulphate fraction showed three main peaks with slight but characteristic differences in the various zones of the normal cartilage plate. Significant shifts in the proportion of these peaks were observed in several pathological biopsies, indicating possible deviations from the normal molecular characteristics of the chondroitin sulphate. Analysis of the main chondroitin sulphate fraction, obtained from pooled samples of normal tibial growth plate after fractionation on the macroscale, indicated that all three peaks contained both chondroitin-4 sulphate and chondroitin-6 sulphate and that they probably differed in their molecular weight.

Sign in / Sign up

Export Citation Format

Share Document