scholarly journals Targeting Corticotropin-Releasing Factor Projections from the Oval Nucleus of the Bed Nucleus of the Stria Terminalis Using Cell-Type Specific Neuronal Tracing Studies in Mouse and Rat Brain

2016 ◽  
Vol 28 (12) ◽  
Author(s):  
J. Dabrowska ◽  
D. Martinon ◽  
M. Moaddab ◽  
D. G. Rainnie
Keyword(s):  
Rat Brain ◽  
Corticotropin Releasing Factor ◽  
Stria Terminalis ◽  
Cell Type ◽  
Neuronal Tracing ◽  
Bed Nucleus ◽  
Oval Nucleus ◽  
Cell Type Specific

scholarly journals Opiate dependence induces cell type-specific plasticity of intrinsic membrane properties in the rat juxtacapsular bed nucleus of stria terminalis (jcBNST)

2017 ◽  
Vol 234 (23-24) ◽  
pp. 3485-3498 ◽  
Author(s):  
Walter Francesconi ◽  
Attila Szücs ◽  
Fulvia Berton ◽  
George F. Koob ◽  
Leandro F. Vendruscolo ◽  
...  
Keyword(s):  
Opiate Dependence ◽  
Membrane Properties ◽  
Stria Terminalis ◽  
Cell Type ◽  
Bed Nucleus ◽  
Cell Type Specific ◽  
Intrinsic Membrane Properties

scholarly journals Evolutionarily Conserved Glucocorticoid Regulation of Corticotropin-Releasing Factor Expression

Endocrinology ◽  
2008 ◽  
Vol 149 (5) ◽  
pp. 2352-2360 ◽  
Author(s):  
Meng Yao ◽  
Jay Schulkin ◽  
Robert J. Denver
Keyword(s):  
Glucocorticoid Receptor ◽  
Paraventricular Nucleus ◽  
Molecular Mechanisms ◽  
Preoptic Area ◽  
Corticotropin Releasing Factor ◽  
Stria Terminalis ◽  
Medial Amygdala ◽  
Cell Type ◽  
Synthesis Inhibitor ◽  
Bed Nucleus

Glucocorticoids (GCs) exert feedback regulation on corticotropin-releasing factor (CRF) neurons in mammals. The nature of GC actions is cell-type specific, being either inhibitory (e.g. paraventricular nucleus) or stimulatory (e.g. amygdala and bed nucleus of the stria terminalis). Nothing is known about differential regulation of CRF gene expression by GCs in nonmammalian vertebrates. We studied the actions of GCs on CRF expression in discrete brain regions of the frog Xenopus laevis. Treatment with corticosterone (CORT) decreased, whereas the corticosteroid synthesis inhibitor metyrapone increased CRF expression in the anterior preoptic area (homolog of the mammalian paraventricular nucleus), as measured by CRF primary transcript, mRNA, and CRF immunoreactivity (ir) (by immunocytochemistry). By contrast to the preoptic area, CORT increased CRF-ir in the medial amygdala and bed nucleus of the stria terminalis, whereas metyrapone decreased CRF-ir in the medial amygdala. CRF-ir and glucocorticoid receptor-ir were colocalized in cells in the frog brain. In transient transfection assays in PC-12 cells, GCs decreased forskolin-induced activation of the frog CRF promoters. Treatment with CORT also reduced CRF promoter activity in transfected tadpole brain in vivo. Frog glucocorticoid receptor bound with high-affinity in vitro to regions in the proximal promoters of frog CRF genes that are homologous with the human CRF gene. Our findings suggest that the neural cell-type specificity and molecular mechanisms of GC-dependent regulation of CRF are phylogenetically ancient, and that the limbic pathways mediating behavioral and physiological responses to stressors were likely present in the earliest land-dwelling vertebrates.

scholarly journals High-resolution and cell-type-specific photostimulation mapping shows weak excitatory vs. strong inhibitory inputs in the bed nucleus of the stria terminalis

2016 ◽  
Vol 115 (6) ◽  
pp. 3204-3216 ◽  
Author(s):  
Xiangmin Xu ◽  
Taruna Ikrar ◽  
Yanjun Sun ◽  
Rommel Santos ◽  
Todd C. Holmes ◽  
...  
Keyword(s):  
High Resolution ◽  
Functional Organization ◽  
Stria Terminalis ◽  
Cell Type ◽  
Experimental Conditions ◽  
Bed Nucleus ◽  
Local Circuit ◽  
Synaptic Inputs ◽  
Cell Type Specific ◽  
Local Circuits

The bed nucleus of the stria terminalis (BNST) is a key component of the extended amygdala and has been implicated in anxiety and addiction. As individual neurons function within neural circuits, it is important to understand local microcircuits and larger network connections of identified neuronal types and understand how maladaptive changes in the BNST neural networks are induced by stress and drug abuse. However, due to limitations of classic anatomical and physiological methods, the local circuit organization of synaptic inputs to specific BNST neuron types is not well understood. In this study, we report on the application of high-resolution and cell-type-specific photostimulation methodology developed in our laboratory to local circuit mapping in the BNST. Under calibrated experimental conditions, laser photostimulation via glutamate uncaging or channelrhodopsin-2 photoactivation evokes spiking of BNST neurons perisomatically, without activating spikes from axons of passage or distal dendrites. Whole cell recordings, combined with spatially restricted photostimulation of presynaptic neurons at many different locations over a large region, allow high-resolution mapping of presynaptic input sources to single recorded neurons in the BNST. We constructed maps of synaptic inputs impinging onto corticotrophin-releasing hormone-expressing (CRH+) BNST neurons in the dorsolateral BNST and found that the CRH+ neurons receive predominant local inhibitory synaptic connections with very weak excitatory connections. Through cell-type-specific optogenetic stimulation mapping, we generated maps of somatostatin-expressing neuron-specific inhibitory inputs to BNST neurons. Taken together, the photostimulation-based techniques offer us powerful tools for determining the functional organization of local circuits of specific BNST neuron types.

scholarly journals Corticotropin-releasing factor neurons in the bed nucleus of the stria terminalis exhibit sex-specific pain encoding in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Waylin Yu ◽  
Christina M. Caira ◽  
Natalia del R. Rivera Sanchez ◽  
Garrett A. Moseley ◽  
Thomas L. Kash
Keyword(s):  
Corticotropin Releasing Factor ◽  
Stria Terminalis ◽  
Noxious Heat ◽  
Specific Expression ◽  
Pain Experience ◽  
Bed Nucleus ◽  
Cell Type Specific ◽  
In Vivo Calcium Imaging ◽  
Pain Regulation

AbstractThe bed nucleus of the stria terminalis (BNST) plays an emerging role in pain regulation. Pharmacological studies have found that inhibiting corticotropin-releasing factor (CRF) signaling in the BNST can selectively mitigate the sensory and affective-motivational components of pain. However, mechanistic insight on the source of CRF that drives BNST responses to these harmful experiences remains unknown. In the present study, we used a series of genetic approaches to show that CRF in the BNST is engaged in the processing and modulation of pain. We conducted cell-type specific in vivo calcium imaging in CRF-Cre mice and found robust and synchronized recruitment of BNSTCRF neurons during acute exposures to noxious heat. Distinct patterns of recruitment were observed by sex, as the magnitude and timing of heat responsive activity in BNSTCRF neurons differed for male and female mice. We then used a viral approach in Floxed-CRF mice to selectively reduce CRF expression in the BNST and found it decreased nociceptive sensitivity for both sexes and increased paw attending for females. Together, these findings reveal that CRF in the BNST influences multiple facets of the pain experience to impact the sex-specific expression of pain-related behaviors.

Anxiety-related cell-type-specific neural circuits in the anterior-dorsal bed nucleus of the stria terminalis

2020 ◽  
Vol 65 (14) ◽  
pp. 1203-1216 ◽  
Author(s):  
Xinxin Wang ◽  
Yongsheng Zhang ◽  
Xu Wang ◽  
Jiaqi Dai ◽  
Ruifang Hua ◽  
...  
Keyword(s):  
Neural Circuits ◽  
Stria Terminalis ◽  
Cell Type ◽  
Bed Nucleus ◽  
Anterior Dorsal ◽  
Related Cell ◽  
Cell Type Specific

Expression of the Ly-6 family proteins Lynx1 and Ly6H in the rat brain is compartmentalized, cell-type specific, and developmentally regulated

2013 ◽  
Vol 219 (6) ◽  
pp. 1923-1934 ◽  
Author(s):  
Morten Skøtt Thomsen ◽  
Betül Cinar ◽  
Majbrit Myrup Jensen ◽  
Ekaterina N. Lyukmanova ◽  
Mikhail A. Shulepko ◽  
...  
Keyword(s):  
Rat Brain ◽  
Cell Type ◽  
Developmentally Regulated ◽  
Cell Type Specific
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