Faster kinetics of quantal catecholamine release in mouse chromaffin cells stimulated with acetylcholine, compared with other secretagogues

2016 ◽  
Vol 139 (5) ◽  
pp. 722-736 ◽  
Author(s):  
Enrique Calvo-Gallardo ◽  
Ángela López-Gil ◽  
Iago Méndez-López ◽  
Carmen Martínez-Ramírez ◽  
Juan Fernando Padín ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Catecholamine Release ◽  
Kinetics Of

Plasmalemmal sodium-calcium exchanger shapes the calcium and exocytotic signals of chromaffin cells at physiological temperature

2013 ◽  
Vol 305 (2) ◽  
pp. C160-C172 ◽  
Author(s):  
Juan-Fernando Padín ◽  
José-Carlos Fernández-Morales ◽  
Román Olivares ◽  
Stefan Vestring ◽  
Juan-Alberto Arranz-Tagarro ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Catecholamine Release ◽  
Sodium Calcium Exchanger ◽  
Physiological Temperature ◽  
Slowing Down ◽  
Sodium Calcium ◽  
High K ◽  
Highly Sensitive ◽  
Bovine Chromaffin Cells ◽  
Kinetics Of

The activity of the plasmalemmal Na+/Ca2+ exchanger (NCX) is highly sensitive to temperature. We took advantage of this fact to explore here the effects of the NCX blocker KB-R7943 (KBR) at 22 and 37°C on the kinetics of Ca2+ currents ( ICa), cytosolic Ca2+ ([Ca2+]c) transients, and catecholamine release from bovine chromaffin cells (BCCs) stimulated with high K+, caffeine, or histamine. At 22°C, the effects of KBR on those parameters were meager or nil. However, at 37°C whereby the NCX is moving Ca2+ at a rate fivefold higher than at 22°C, various of the effects of KBR were pronounced, namely: 1) no effects on ICa; 2) reduction of the [Ca2+]c transient amplitude and slowing down of its rate of clearance; 3) blockade of the K+-elicited quantal release of catecholamine; 4) blockade of burst catecholamine release elicited by K+; 5) no effect on catecholamine release elicited by short K+ pulses (1–2 s) and blockade of the responses produced by longer K+ pulses (3–5 s); and 6) potentiation of secretion elicited by histamine or caffeine. Furthermore, the more selective NCX blocker SEA0400 also potentiated the secretory responses to caffeine. The results suggest that at physiological temperature the NCX substantially contributes to shaping the kinetics of [Ca2+]c transients and the exocytotic responses elicited by Ca2+ entry through Ca2+ channels as well as by Ca2+ release from the endoplasmic reticulum.

scholarly journals Influence of Quantal Size and cAMP on the Kinetics of Quantal Catecholamine Release from Rat Chromaffin Cells

2007 ◽  
Vol 92 (8) ◽  
pp. 2735-2746 ◽  
Author(s):  
Kim San Tang ◽  
Nan Wang ◽  
Amy Tse ◽  
Frederick W. Tse
Keyword(s):  
Chromaffin Cells ◽  
Catecholamine Release ◽  
Quantal Size ◽  
Kinetics Of

Dual role of calbindin-D28Kin vesicular catecholamine release from mouse chromaffin cells

2006 ◽  
Vol 99 (2) ◽  
pp. 628-640 ◽  
Author(s):  
R. H. S. Westerink ◽  
M. B. Rook ◽  
J. P. Beekwilder ◽  
W. J. Wadman
Keyword(s):  
Chromaffin Cells ◽  
Dual Role ◽  
Catecholamine Release

Catecholamine Release Modulation by Adenosine Through A2a Receptors in Mouse Chromaffin Cells in Culture

2014 ◽  
pp. 244-245
Author(s):  
Magda M. Santana ◽  
Joana Rosmaninho-Salgado ◽  
Manuella Kaster ◽  
Vera Cortez ◽  
Rodrigo A. Cunha ◽  
...  
Keyword(s):  
Chromaffin Cells ◽  
Catecholamine Release ◽  
A2a Receptors ◽  
Cells In Culture
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