scholarly journals Molecular cloning, epigenetic regulation, and functional characterization of Prkd1 gene promoter in dopaminergic cell culture models of Parkinson's disease

2015 ◽  
Vol 135 (2) ◽  
pp. 402-415 ◽  
Author(s):  
Muhammet Ay ◽  
Huajun Jin ◽  
Dilshan S. Harischandra ◽  
Arunkumar Asaithambi ◽  
Arthi Kanthasamy ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Cell Culture ◽  
Molecular Cloning ◽  
Epigenetic Regulation ◽  
Functional Characterization ◽  
Gene Promoter ◽  
Dopaminergic Cell ◽  
Culture Models ◽  
Cell Culture Models

The regulatory elements of PLZF gene are not conserved as reveled by molecular cloning and functional characterization of PLZF gene promoter of Clarias batrachus

Gene Reports ◽  
2019 ◽  
Vol 16 ◽  
pp. 100402
Author(s):  
Swapnarani Nayak ◽  
Lipika Patnaik ◽  
Meenati Manjari Soren ◽  
V. Chakrapani ◽  
Shibani Dutta Mohapatra ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
Gene Promoter ◽  
Regulatory Elements ◽  
Clarias Batrachus

Molecular cloning and functional characterization of a seed-specific VvβVPE gene promoter from Vitis vinifera

Planta ◽  
2019 ◽  
Vol 250 (2) ◽  
pp. 657-665 ◽  
Author(s):  
Peijie Gong ◽  
Rong Wei ◽  
Yan Li ◽  
Ruipu Wang ◽  
Yujin Tang ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Molecular Cloning ◽  
Functional Characterization ◽  
Gene Promoter

scholarly journals Electron microscopy characterization of minerals formed in vitro by human bone cells and vascular smooth muscle cells

2019 ◽  
Author(s):  
Elena Tsolaki ◽  
Louis Didierlaurent ◽  
Eike Müller ◽  
Markus Rottmar ◽  
Najma Latif ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Smooth Muscle ◽  
Soft Tissue ◽  
Vascular Smooth Muscle Cells ◽  
Bone Cells ◽  
Culture Models ◽  
Cell Culture Models

AbstractSoft tissue mineralization has been found to be a major component of diseases such as aortic valve stenosis and rheumatic heart disease. Cardiovascular mineralization has been suggested to follow mechanisms similar to those of bone formation with several cell culture models been developed over the years to provide mechanistic insights. These cell models have been characterized by a wide range of biochemical and molecular methods, which identified the presence of osteogenic markers and bone-like cells. However, there is a surprisingly small number of studies where the mineral formed in these cell culture models has been characterized by physico-chemical methods, and even fewer studies have compared this mineral to the one produced by bone cells in cultures. Here we investigated the morphology and composition of the minerals formed in cell cultures of vascular smooth muscle cells and bone cells. Electron microscopy and traditional cell mineralization assays were applied, revealing that vascular cells are indeed able to form calcified nodules of elemental composition similar to bone, however with different morphology. Comparison of morphologies of the two minerals to that found in cardiovascular tissue shows that some of tissue calcification resembles the calcified fibers produced by bone cells in vitro. These results suggest that the characterization of the mineral is of utmost importance and its morphology and chemical properties can contribute an important piece of information in the comprehensive analysis of soft tissue mineralization mechanisms, both in in vitro cell culture as well as in clinical samples.

scholarly journals PINK1 Defect Causes Mitochondrial Dysfunction, Proteasomal Deficit and α-Synuclein Aggregation in Cell Culture Models of Parkinson's Disease

PLoS ONE ◽  
2009 ◽  
Vol 4 (2) ◽  
pp. e4597 ◽  
Author(s):  
Wencheng Liu ◽  
Cristofol Vives-Bauza ◽  
Rebeca Acín-Peréz- ◽  
Ai Yamamoto ◽  
Yingcai Tan ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Cell Culture ◽  
Parkinson's Disease ◽  
Mitochondrial Dysfunction ◽  
Culture Models ◽  
Cell Culture Models

Validation of in vitro cell culture models of the blood–brain barrier: Tightness characterization of two promising cell lines

2008 ◽  
Vol 97 (12) ◽  
pp. 5158-5175 ◽  
Author(s):  
Winfried Neuhaus ◽  
Verena E. Plattner ◽  
Michael Wirth ◽  
Bettina Germann ◽  
Bodo Lachmann ◽  
...  
Keyword(s):  
Cell Culture ◽  
Blood Brain Barrier ◽  
Cell Lines ◽  
Brain Barrier ◽  
In Vitro Cell Culture ◽  
Blood Brain ◽  
Culture Models ◽  
Cell Culture Models
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