scholarly journals Induction of serpinb1a by PACAP or NGF is required for PC12 cells survival after serum withdrawal

2014 ◽  
Vol 131 (1) ◽  
pp. 21-32 ◽  
Author(s):  
Tommy Seaborn ◽  
Aurélia Ravni ◽  
Ruby Au ◽  
Bill K. C. Chow ◽  
Alain Fournier ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Serum Withdrawal

scholarly journals Internucleosomal DNA cleavage and neuronal cell survival/death

1993 ◽  
Vol 122 (3) ◽  
pp. 523-532 ◽  
Author(s):  
A Batistatou ◽  
LA Greene
Keyword(s):  
Cell Death ◽  
Cell Survival ◽  
Pc12 Cells ◽  
Dna Fragmentation ◽  
Cell Cultures ◽  
Pc12 Cell ◽  
Dna Cleavage ◽  
Sympathetic Neurons ◽  
Term Survival ◽  
Serum Withdrawal

Serum-free PC12 cell cultures have been used to study the mechanisms of neuronal death after neurotrophic factor deprivation. We previously reported that PC12 cells undergo "apoptotic" internucleosomal DNA cleavage after withdrawal of trophic support. Here, we have used a sensitive method to detect PC12 cell DNA fragmentation within three hrs of serum removal and have exploited this assay to examine several aspects regarding the mechanisms of neuronal survival/death. Major advantages of this assay are that it permits acute experiments to be performed well before other manifest signs of cell death and under conditions that cannot be applied chronically. We find that this apopotic DNA fragmentation is distinct from the random DNA degradation that occurs during necrotic death. Major observations include the following: (a) There is a good correlation between the ability of trophic substances to promote PC12 cell survival and to inhibit early DNA fragmentation. (b) Phorbol ester, an activator of PKC, acutely suppresses DNA fragmentation, but does not promote long-term survival or inhibition of endonuclease activity when applied chronically due to its downregulation of PKC. (c) Cells undergoing apoptosis within 3 h of serum withdrawal have a "commitment point" of only 1.0-1.5 h beyond which they can no longer be rescued by NGF. (d) Aurin, a non-carboxylic analog of the endonuclease inhibitor ATA, also inhibits DNA fragmentation and promotes short-term survival of PC12 cells. (e) Macromolecular synthesis is not required for DNA fragmentation or for NGF to prevent this event. (f) Extracellular Ca2+ is not required for internucleosomal DNA cleavage caused by serum withdrawal or for suppression of this by NGF. (g) DNA fragmentation can also be detected in cultures of rat sympathetic neurons as early as 10 h after removal of NGF. As in PC12 cell cultures, this precedes morphological signs of cell death.

Protective effects of atypical antipsychotic drugs on PC12 cells after serum withdrawal

2002 ◽  
Vol 69 (2) ◽  
pp. 278-283 ◽  
Author(s):  
Ou Bai ◽  
Zelan Wei ◽  
Wenfu Lu ◽  
Rudy Bowen ◽  
David Keegan ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Atypical Antipsychotic ◽  
Antipsychotic Drugs ◽  
Protective Effects ◽  
Serum Withdrawal ◽  
Atypical Antipsychotic Drugs

Green tea polyphenol (−)-epigallocatechin-3-gallate protects rat PC12 cells from apoptosis induced by serum withdrawal

2003 ◽  
Vol 5 (6) ◽  
pp. 419-424 ◽  
Author(s):  
Silvia Mandel ◽  
Lydia Reznichenko ◽  
Tamar Amit ◽  
Moussa B. H. Youdim
Keyword(s):  
Pc12 Cells ◽  
Green Tea ◽  
Tea Polyphenol ◽  
Serum Withdrawal ◽  
Green Tea Polyphenol

The influence of DHEA on NGF-induced differentiation of pheochromocytoma PC12 cells

2006 ◽  
Vol 114 (S 1) ◽  
Author(s):  
CG Ziegler ◽  
AW Krug ◽  
F Sicard ◽  
S Sperber ◽  
M Ehrhart-Bornstein ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Induced Differentiation ◽  
Pheochromocytoma Pc12 ◽  
Pheochromocytoma Pc12 Cells

Hyperforin mediates neurite outgrowth via TRPC6 channels in PC12 cells

2007 ◽  
Vol 40 (05) ◽  
Author(s):  
K Leuner ◽  
M Müller ◽  
V Kasanzki ◽  
C Harteneck ◽  
WE Müller
Keyword(s):  
Neurite Outgrowth ◽  
Pc12 Cells
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