scholarly journals Functional analysis and purification of a Pen-2 fusion protein for γ-secretase structural studies

2014 ◽  
Vol 131 (1) ◽  
pp. 94-100
Author(s):  
Oliver Holmes ◽  
Swetha Paturi ◽  
Michael S. Wolfe ◽  
Dennis J. Selkoe
Keyword(s):  
Functional Analysis ◽  
Fusion Protein ◽  
Structural Studies

scholarly journals Structure-Function of Falcipains: Malarial Cysteine Proteases

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Kailash C. Pandey ◽  
Rajnikant Dixit
Keyword(s):  
Functional Analysis ◽  
Drug Development ◽  
Protease Inhibitors ◽  
Cysteine Protease ◽  
Essential Role ◽  
Cysteine Proteases ◽  
Structural Studies ◽  
Cysteine Protease Inhibitors ◽  
Hemoglobin Binding ◽  
Structural Insight

Evidence indicates that cysteine proteases play essential role in malaria parasites; therefore an obvious area of investigation is the inhibition of these enzymes to treat malaria. Studies with cysteine protease inhibitors and manipulating cysteine proteases genes have suggested a role for cysteine proteases in hemoglobin hydrolysis. The best characterizedPlasmodiumcysteine proteases are falcipains, which are papain family enzymes. Falcipain-2 and falcipain-3 are major hemoglobinases ofP. falciparum. Structural and functional analysis of falcipains showed that they have unique domains including a refolding domain and a hemoglobin binding domain. Overall, the complexes of falcipain-2 and falcipain-3 with small and macromolecular inhibitors provide structural insight to facilitate the design or modification of effective drug treatment against malaria. Drug development targeting falcipains should be aided by a strong foundation of biochemical and structural studies.

scholarly journals Functional Analysis of a Conserved Region of the Baculovirus Envelope Fusion Protein, LD130

Virology ◽  
2002 ◽  
Vol 304 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Margot N. Pearson ◽  
Rebecca L.Q. Russell ◽  
George F. Rohrmann
Keyword(s):  
Functional Analysis ◽  
Fusion Protein ◽  
Conserved Region

Regulation of T cell responses by BTLA: Functional analysis using a soluble BTLAIg fusion protein

2004 ◽  
Vol 2004 (Fall) ◽  
Author(s):  
Bernd Lepenies ◽  
Bernhard Fleischer ◽  
Thomas Jacobs
Keyword(s):  
Functional Analysis ◽  
T Cell ◽  
Fusion Protein ◽  
T Cell Responses ◽  
Cell Responses

scholarly journals Species-Specific Functional Regions of the Green Alga Gamete Fusion Protein HAP2 Revealed by Structural Studies

Structure ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 113-124.e4 ◽  
Author(s):  
Eduard Baquero ◽  
Juliette Fedry ◽  
Pierre Legrand ◽  
Thomas Krey ◽  
Felix A. Rey
Keyword(s):  
Fusion Protein ◽  
Green Alga ◽  
Structural Studies ◽  
Gamete Fusion ◽  
Functional Regions ◽  
Species Specific

The Saccharomyces cerevisiae ADP/ATP carrier-iso 1 cytochrome c fusion protein: One-step purification and functional analysis in vitro

Biochimie ◽  
2007 ◽  
Vol 89 (9) ◽  
pp. 1070-1079 ◽  
Author(s):  
M REY ◽  
X BRAZZOLOTTO ◽  
B CLEMENCON ◽  
A AFCHAIN ◽  
G BRANDOLIN ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Analysis ◽  
Cytochrome C ◽  
Fusion Protein ◽  
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