scholarly journals Guanosine controls inflammatory pathways to afford neuroprotection of hippocampal slices under oxygen and glucose deprivation conditions

2013 ◽  
Vol 126 (4) ◽  
pp. 437-450 ◽  
Author(s):  
Tharine Dal-Cim ◽  
Fabiana K. Ludka ◽  
Wagner C. Martins ◽  
Charlise Reginato ◽  
Esther Parada ◽  
...  
Keyword(s):  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Oxygen And Glucose Deprivation ◽  
Inflammatory Pathways

scholarly journals Molecular and Pharmacological Modulation of CALHM1 Promote Neuroprotection against Oxygen and Glucose Deprivation in a Model of Hippocampal Slices

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 664 ◽  
Author(s):  
Javier Garrosa ◽  
Iñigo Paredes ◽  
Philippe Marambaud ◽  
Manuela G. López ◽  
María F. Cano-Abad
Keyword(s):  
Neuroprotective Effect ◽  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Point Of View ◽  
Oxygen And Glucose Deprivation ◽  
Molecular Events ◽  
Vitro Model ◽  
Species Production ◽  
Reperfusion Phase

Calcium homeostasis modulator 1 (CALHM1) is a calcium channel involved in the regulation of cytosolic Ca2+ levels. From a physiological point of view, the open state of CALHM1 depends not only on voltage but also on the extracellular concentration of calcium ([Ca2+]) ions. At low [Ca2+]e or depolarization, the channel is opened, allowing Ca2+ influx; however, high extracellular [Ca2+]e or hyperpolarization promote its resting state. The unique Ca2+ permeation of CALHM1 relates to the molecular events that take place in brain ischemia, such as depolarization and extracellular changes in [Ca2+]e, particularly during the reperfusion phase after the ischemic insult. In this study, we attempted to understand its role in an in vitro model of ischemia, namely oxygen and glucose deprivation, followed by reoxygenation (OGD/Reox). To this end, hippocampal slices from wild-type Calhm1+/+, Calhm1+/−, and Calhm1−/− mice were subjected to OGD/Reox. Our results point out to a neuroprotective effect when CALHM1 is partially or totally absent. Pharmacological manipulation of CALHM1 with CGP37157 reduced cell death in Calhm1+/+ slices but not in that of Calhm1−/− mice after exposure to the OGD/Reox protocol. This ionic protection was also verified by measuring reactive oxygen species production upon OGD/Reox in Calhm1+/+ and Calhm1−/− mice, resulting in a downregulation of ROS production in Calhm1−/− hippocampal slices and increased expression of HIF-1α. Taken together, we can conclude that genetic or pharmacological inhibition of CALHM1 results in a neuroprotective effect against ischemia, due to an attenuation of the neuronal calcium overload and downregulation of oxygen reactive species production.

scholarly journals Purine receptors are required for DHA-mediated neuroprotection against oxygen and glucose deprivation in hippocampal slices

2014 ◽  
Vol 11 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Simone Molz ◽  
Gislaine Olescowicz ◽  
Jessica Rossana Kraus ◽  
Fabiana Kalyne Ludka ◽  
Carla I. Tasca
Keyword(s):  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Purine Receptors ◽  
Oxygen And Glucose Deprivation

Acute and repeated restraint stress influences cellular damage in rat hippocampal slices exposed to oxygen and glucose deprivation

2005 ◽  
Vol 65 (5) ◽  
pp. 443-450 ◽  
Author(s):  
Fernanda Urruth Fontella ◽  
Helena Cimarosti ◽  
Leonardo Machado Crema ◽  
Ana Paula Thomazi ◽  
Marina Concli Leite ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Cellular Damage ◽  
Oxygen And Glucose Deprivation ◽  
Repeated Restraint Stress ◽  
Stress Influences

Mechanisms of 1S,3R-ACPD-induced neuroprotection in rat hippocampal slices subjected to oxygen and glucose deprivation

1996 ◽  
Vol 35 (8) ◽  
pp. 1037-1048 ◽  
Author(s):  
D.L. Small ◽  
R. Monette ◽  
B. Chakravarthy ◽  
J. Durkin ◽  
G. Barbe ◽  
...  
Keyword(s):  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Oxygen And Glucose Deprivation

Melatonin protects against oxygen and glucose deprivation by decreasing extracellular glutamate and Nox-derived ROS in rat hippocampal slices

2016 ◽  
Vol 57 ◽  
pp. 61-68 ◽  
Author(s):  
Paloma Patiño ◽  
Esther Parada ◽  
Victor Farré-Alins ◽  
Simone Molz ◽  
Ramón Cacabelos ◽  
...  
Keyword(s):  
Hippocampal Slices ◽  
Glucose Deprivation ◽  
Extracellular Glutamate ◽  
Oxygen And Glucose Deprivation
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