A novel method to analysein vivothe physiological state and cell viability of phototrophic microorganisms by confocal laser scanning microscopy using a dual laser

2017 ◽  
Vol 268 (1) ◽  
pp. 53-65 ◽  
Author(s):  
LAIA MILLACH ◽  
ALEIX OBIOL ◽  
ANTONIO SOLÉ ◽  
ISABEL ESTEVE
Keyword(s):  
Cell Viability ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Physiological State ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Phototrophic Microorganisms ◽  
Novel Method ◽  
Dual Laser

Combined Confocal Laser Scanning Microscopy Techniques for A Rapid Assessment of the Effect and Cell Viability of Scenedesmus sp. DE2009 Under Metal Stress

2019 ◽  
Vol 25 (4) ◽  
pp. 998-1003 ◽  
Author(s):  
Laia Millach ◽  
Eduard Villagrasa ◽  
Antonio Solé ◽  
Isabel Esteve
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Heterotrophic Bacteria ◽  
Physiological State ◽  
Laser Scanning Microscopy ◽  
Metal Stress ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Pigment Analysis

AbstractPhototrophic microorganisms are the dominant populations in microbial mats, which play an important role in stabilizing sediments, such as happens in the Ebro Delta. These microorganisms are exposed to low metal concentrations over a long period of time. Distinct methods have been used to evaluate their toxic effect on the preservation of these ecosystems. Nevertheless, most of these techniques are difficult to apply in isolated phototrophs because (i) they usually form consortia with heterotrophic bacteria, (ii) are difficult to obtain in axenic cultures, and (iii) do not grow on solid media.In this study, and for the first time, a combination of fast, non-invasive, and in vivo Confocal Laser Scanning Microscopy (CLSM) techniques were applied in a consortium of Scenedesmus sp. DE2009 to analyze its physiological state and viability under metal stress conditions. Microalga was more resistant to Pb followed by Cr and Cu. However, in multimetal combinations, the presence of Cu negatively affected microalga growth. Additionally, the inhibitory concentration (IC) values were also calculated by CLSM pigment analysis. The result determines a higher degree of toxicity for Cu and Cr in comparison to Pb. The high sensitivity of these CLSM-methods to detect low concentrations allows consideration of Scenedesmus sp. DE2009 as a good bioindicator of metal pollution in natural environments.

scholarly journals Autofluorescence as a Tool for Structural Analysis of Biofilms Formed by Nonpigmented Rapidly Growing Mycobacteria

2012 ◽  
Vol 79 (3) ◽  
pp. 1065-1067 ◽  
Author(s):  
María-Carmen Muñoz-Egea ◽  
María García-Pedrazuela ◽  
Ignacio Mahillo ◽  
María Jesús García ◽  
Jaime Esteban
Keyword(s):  
Image Analysis ◽  
Structural Analysis ◽  
Cell Viability ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Rapidly Growing Mycobacteria

ABSTRACTThe structure of biofilms formed by seven nonpigmented rapidly growing mycobacteria, including saprophytes and opportunistic species, was analyzed. Analysis included amount of covered surface, thickness, cell viability, and presence of intrinsic autofluorescence at different times using confocal laser scanning microscopy and image analysis. Autofluorescence was detected inside and outside cells of all mycobacteria.

ACTIVATED SLUDGE FLOC CHARACTERIZATION BY CONFOCAL LASER SCANNING MICROSCOPY

2012 ◽  
Vol 11 (3) ◽  
pp. 669-674 ◽  
Author(s):  
Szabolcs Szilveszter ◽  
Botond Raduly ◽  
Szilard Bucs ◽  
Beata Abraham ◽  
Szabolcs Lanyi ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser

Cuticular structures in the copulatory apparatus of Planorbis planorbis (Linnaeus, 1758) (Gastropoda: Pulmonata: Planorbidae)

2009 ◽  
Vol 18 (1) ◽  
pp. 11-16
Author(s):  
E.V. Soldatenko ◽  
A.A. Petrov
Keyword(s):  
Light Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Taxonomic Status ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Copulatory Apparatus ◽  
The Family ◽  
Cuticular Structures

The morphology of the copulatory apparatus and associated cuticular structures in Planorbis planorbis was studied by light microscopy, SEM, TEM and confocal laser scanning microscopy. The significance of these cuticular structures for the taxonomic status of the species and for the systematics of the family Planorbidae in general is discussed.

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