Oolong tea extract as a substitute for uranyl acetate in staining of ultrathin sections based on examples of animal tissues for transmission electron microscopy

2017 ◽  
Vol 267 (1) ◽  
pp. 27-33 ◽  
Author(s):  
XIAOHUA HE ◽  
BIN LIU
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Uranyl Acetate ◽  
Animal Tissues ◽  
Oolong Tea ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Tea Extract

The Ultrastructure of Lymphocytic Hypophysitis

1981 ◽  
Vol 39 ◽  
pp. 466-467
Author(s):  
S.L. Asa ◽  
K. Kovacs ◽  
J. M. Bilbao ◽  
R. G. Josse ◽  
K. Kreines
Keyword(s):  
Electron Microscopy ◽  
Plasma Cells ◽  
Secretory Granules ◽  
Sella Turcica ◽  
Uranyl Acetate ◽  
Lymphocytic Hypophysitis ◽  
Pituitary Cells ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Mass Lesions

Seven cases of lymphocytic hypophysitis in women have been reported previously in association with various degrees of hypopituitarism. We report two pregnant patients who presented with mass lesions of the sella turcica, clinically mimicking pituitary adenoma. However, pathologic examination revealed extensive infiltration of the anterior pituitary by lymphocytes and plasma cells with destruction of the gland. To our knowledge, the ultrastructural features of lymphocytic hypophysitis have not been studied so far.For transmission electron microscopy, tissue from surgical specimens was fixed in glutaraldehyde, postfixed in OsO4, dehydrated and embedded in epoxy-resin. Ultrathin sections were stained with uranyl acetate and lead citrate and examined with a Philips 300 electron microscope.Electron microscopy revealed adenohypophysial cells of all types exhibiting varying degrees of injury. In the areas of most dense inflammatory cell infiltration pituitary cells contained large lysosomal bodies fusing with secretory granules (Fig. 1), as well as increased numbers of swollen mitochondria, indicating oncocytic transformation (Fig. 2).

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

Oolong tea extract as a substitute for uranyl acetate in staining of ultrathin sections

2008 ◽  
Vol 229 (1) ◽  
pp. 17-20 ◽  
Author(s):  
S. SATO ◽  
A. ADACHI ◽  
Y. SASAKI ◽  
M. GHAZIZADEH
Keyword(s):  
Uranyl Acetate ◽  
Oolong Tea ◽  
Ultrathin Sections ◽  
Tea Extract

Postero-Lateral Glands of Temnocephala Minor Haswell, 1888 (Platyhelminthes: Temnocephalida)

1996 ◽  
Vol 44 (1) ◽  
pp. 69
Author(s):  
LRG Cannon ◽  
NA Warson
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Lateral Margin ◽  
Freshwater Crayfish ◽  
Cherax Destructor ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Whole Mounts

Temnocephala minor Haswell, 1888 lives ectosymbiotically on the surface of the freshwater crayfish Cherax destructor in the Murray-Darling drainages of Australia. Some glands open on the postero-lateral margin and, being moderately refractory to many stains, can be overlooked in whole mounts and sections, and were, in fact, missed by Haswell. Observations were made on living worms with intra vitam dyes, and on whole mounts, wax sections and ultrathin sections using transmission electron microscopy (TEM) to characterise the secretion from these glands and ascertain its mode of manufacture. The function of the glands remains unknown although it appears non-adhesive.

The Technology in Sample Preparation for Transmission Electron Microscopy and Ultrastructure Observation of Lung Tissue in Rats with Experimental Silicosis

2014 ◽  
Vol 875-877 ◽  
pp. 695-698
Author(s):  
Qian Li ◽  
Li Juan Zhang ◽  
Fang Yang ◽  
Wen Li Zhang ◽  
Hong Xu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Success Rate ◽  
Cutting Speed ◽  
Ultrastructural Changes ◽  
Histological Changes ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Perfusion Fixation

ts hard to get ideal ultrathin sections because of the adamant SiO2 dust in silicosis, after perfusion fixation methods and strict control of the cutting speed, improving the success rate of the Silicosis tissue TEM sample preparation of ultrathin sections,so we can more clearly and accurately observed ultrastructural changes of silicosis,and it also can offer morphological basis for research the silicosis organizations function histological changes.

Transmission Electron Microscopy Study of Listeria monocytogenes Treated with Nisin in Combination with either Grape Seed or Green Tea Extract

2008 ◽  
Vol 71 (10) ◽  
pp. 2105-2109 ◽  
Author(s):  
T. SIVAROOBAN ◽  
N. S. HETTIARACHCHY ◽  
M. G. JOHNSON
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Listeria Monocytogenes ◽  
Green Tea ◽  
Morphological Changes ◽  
Grape Seed ◽  
Green Tea Extract ◽  
Transmission Electron ◽  
Phenolic Constituents ◽  
Tea Extract

The objective of this study was to use transmission electron microscopy to investigate the morphological changes that occurred in Listeria monocytogenes cells treated with grape seed extract (GSE), green tea extract (GTE), nisin, and combinations of nisin with either GSE or GTE. The test solutions were prepared with (i) 1% GSE, 1% GTE, 6,400 IU of nisin, and the combination of these dilutions with nisin or with (ii) the pure major phenolic constituents of GSE (0.02% epicatechin plus 0.02% catechin) or GTE (0.02% epicatechin plus 0.02% caffeic acid) and their combinations with 6,400 IU of nisin in tryptic soy broth with 0.6% yeast extract (TSBYE). Test solutions were inoculated with L. monocytogenes at approximately 106 CFU/ml and incubated for 3 or 24 h at 37°C. After 3 h of incubation, cells were harvested and evaluated under a transmission electron microscope (JEOL-100 CX) operating at 80 kV (50,000×). Microscopic examination revealed an altered cell membrane and condensed cytoplasm when L. monocytogenes cells were exposed to a combination of nisin with either GSE or GTE or to pure compounds of the major phenolic constituents in combination. After 24 h of incubation at 37°C, the combinations of nisin with GSE and nisin with GTE reduced the L. monocytogenes population to undetectable levels and 3.7 log CFU/ml, respectively. These observations indicate that the combination of nisin with either GSE or GTE had a synergistic effect, and the combinations of nisin with the major phenolic constituents were most likely associated with the L. monocytogenes cell damage during inactivation in TSBYE at 37°C.

Scanning and Transmission Electron Microscopy of Human Lung in a Patient with Sarcoid-like Reaction

1974 ◽  
Vol 32 ◽  
pp. 14-15
Author(s):  
John H. L. Watson ◽  
Jessica Goodwin ◽  
E. Osborne Coates
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Hypersensitivity Reaction ◽  
Light Microscopy ◽  
Critical Point ◽  
Clinical Evidence ◽  
Human Lung ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Transmission Electron

Biopsies of lung were taken at operation from a patient with semi-acute diffuse pulmonary infiltrates for study by TEM and SEM. Tissue by light microscopy showed non-caseating granulomas consistent with sarcoidosis. Clinical evidence suggested a hypersensitivity reaction related to inhalation of substance of undetermined nature. Samples were fixed in glutaraldehyde, cacodylate-buffered. They were critical point dried and coated with Au-Pd for SEM, and were handled appropriately for TEM in Araldite. Sections were contrasted with uranyl acetate and lead citrate.

Sign in / Sign up

Export Citation Format

Share Document