Multifractal characterization of morphology of human red blood cells membrane skeleton

2015 ◽  
Vol 262 (1) ◽  
pp. 59-72 ◽  
Author(s):  
Ş. ŢĂLU ◽  
S. STACH ◽  
M. KACZMARSKA ◽  
M. FORNAL ◽  
T. GRODZICKI ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Membrane Skeleton ◽  
Human Red Blood Cells

scholarly journals Anti-Trypanosoma cruzi activity, cytotoxicity and, chemical characterization of extracts from seeds of Lonchocarpus cultratus

2021 ◽  
Vol 15 (02) ◽  
pp. 270-279
Author(s):  
Aline Griebler ◽  
Fernanda Weyand Banhuk ◽  
Izabela Virginia Staffen ◽  
Aline Antunes Maciel Bortoluzzi ◽  
Thaís Soprani Ayala ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Methanolic Extract ◽  
Chemical Characterization ◽  
Peritoneal Macrophages ◽  
Chemical Profile ◽  
Murine Macrophages ◽  
Human Red Blood Cells

Introduction: Trypanosoma cruzi is the agent of Chagas’ disease and affects approximately 6-8 million people worldwide. The search for new anti-T. cruzi drugs are relevant because only two drugs exist actually. The objective of this study was to investigate the effect of the extracts from the seeds of Lonchocarpus cultratus on T. cruzi, its cytotoxicity as well as to elucidate its chemical profile. Methodology: The characterization of the extracts was done using 1H-RMN. T. cruzi forms were treated with increasing concentrations of the extracts and after, the percentage of inhibition and IC50 or LC50 were calculated. Murine peritoneal macrophages were treated with different concentrations of the extracts to evaluate the cellular viability. The hemotoxicity was accessed by verifying the levels of hemolysis caused by the extracts on human red blood cells. Results: Chalcones isocordoin and lonchocarpin were detected in the dichloromethane extract, and chalcone lonchocarpin was detected in the hexane extract. The dichloromethane extract showed higher activity against all the forms of T. cruzi compared to the other two extracts, but the hexane showed the best selectivity index. The cytotoxicity observed in murine macrophages was confirmed in human erythrocytes, with dichloromethane extract having the highest toxicity. The methanolic extract showed the lowest anti-T. cruzi activity but was nontoxic to peritoneal murine macrophages and red blood cells. Conclusions: L. cultratus extracts have the potential to be explored for the development of new anti-trypanosomal drugs. This study was the first to demonstrate the action of extracts from the genus Lonchocarpus on infecting forms of T. cruzi.

scholarly journals Isolation and characterization of an age-related antigen present on senescent human red blood cells

Blood ◽  
1981 ◽  
Vol 58 (2) ◽  
pp. 341-349
Author(s):  
EM Alderman ◽  
HH Fudenberg ◽  
RE Lovins
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Double Immunodiffusion ◽  
Specific Density ◽  
Human Red Blood Cells ◽  
Rbc Membrane ◽  
Isolation And Characterization ◽  
Age Related ◽  
Membrane Bound

Autologous membrane-bound IgG was isolated from a subpopulation of human red blood cells (RBC) with specific density greater than 1.110, by affinity chromatography of purified RBC membrane glycoprotein preparations using immobilized wheat germ agglutinin and immobilized anti-human immunoglobulin (Ig) as immunoabsorbents. The Ig-containing population thus obtained, when further separated by chromatography on Sephadex G-200 in the presence of chaotropic agents, yielded four peaks (Ia, Ib, II, and III). Double immunodiffusion revealed the presence of Ig in the first three peaks (IgM in peak Ia, IgA in Ib, and IgG in II) but not in peak III. Peak III was precipitated by the Ig-containing peaks (Ia, Ib, and II) in immunodiffusion assays, suggesting that the antigenic membrane determinants responsible for the binding of autologous Ig to senescent human RBC were contained in this peak (III). Peaks Ia, Ib and II precipitate purified asialoglycophorin; peak III was reactive with purified autoantibodies directed against asialoglycophorin. These results suggest that an age-related antigenic determinant(s) present on senescent human RBC is exposed by desialylation of the major sialoglycoprotein component of the RBC membrane.

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