scholarly journals Combining quantitative 2D and 3D image analysis in the serial block face SEM: application to secretory organelles of pancreatic islet cells

2015 ◽  
Vol 259 (2) ◽  
pp. 155-164 ◽  
Author(s):  
A. SHOMORONY ◽  
C.R. PFEIFER ◽  
M.A. ARONOVA ◽  
G. ZHANG ◽  
T. CAI ◽  
...  
Keyword(s):  
Image Analysis ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
3D Image ◽  
3D Image Analysis ◽  
Block Face ◽  
2D And 3D

Granulometry of bread crumb grain: Contributions of 2D and 3D image analysis at different scale

2007 ◽  
Vol 40 (8) ◽  
pp. 1087-1097 ◽  
Author(s):  
Nejla Lassoued ◽  
Perrine Babin ◽  
Guy Della Valle ◽  
Marie-Françoise Devaux ◽  
Anne-Laure Réguerre
Keyword(s):  
Image Analysis ◽  
3D Image ◽  
Bread Crumb ◽  
3D Image Analysis ◽  
2D And 3D ◽  
Crumb Grain

scholarly journals Video image analysis of labile zinc in viable pancreatic islet cells using a specific fluorescent probe for zinc.

1994 ◽  
Vol 42 (7) ◽  
pp. 877-884 ◽  
Author(s):  
P D Zalewski ◽  
S H Millard ◽  
I J Forbes ◽  
O Kapaniris ◽  
A Slavotinek ◽  
...  
Keyword(s):  
Image Analysis ◽  
Pancreatic Islet ◽  
Fluorescence Intensity ◽  
Islet Cells ◽  
Video Image ◽  
Zinc Homeostasis ◽  
Pancreatic Acinar Cells ◽  
Pancreatic Islet Cells ◽  
Video Image Analysis ◽  
Insulinoma Cells

We used an intracellular zinc-specific fluorophore, Zinquin, in conjunction with fluorescence video image analysis, to reveal labile zinc in pancreatic islet cells, which concentrate this metal for use in synthesis, storage, and secretion of insulin. Zinquin vividly demonstrated zinc in the islet cell secretory granules, which formed a brightly labeled crescent in the cytoplasm between one side of the nucleus and the plasma membrane. Lower but still appreciable amounts of zinc were detected in the remaining cytoplasm, but there was little labeling in the nucleus. Fluorescence intensity varied among islet cells, suggesting differences in zinc content. Their average fluorescence intensity greatly surpassed that of the surrounding pancreatic acinar cells in frozen sections of pancreas and in all other types of cell studied, including lymphocytes, neutrophils, fibroblasts, and erythrocytes. Less labile zinc was detected in cells of the mouse insulinoma cell line NIT-1, regardless of whether they were maintained in long-term culture in the presence or absence of exogenous extracellular zinc. Exposure of islet or insulinoma cells to a high concentration of glucose or other secretagogue decreased the content of labile zinc. Zinquin should be a useful probe for revealing changes in zinc homeostasis in islet B-cells that may be important in their dysfunction and death during diabetes.

2D and 3D Image Analysis by Moments

2016 ◽  
Author(s):  
Jan Flusser ◽  
Tomáš Suk ◽  
Barbara Zitová
Keyword(s):  
Image Analysis ◽  
3D Image ◽  
3D Image Analysis ◽  
2D And 3D

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures
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