scholarly journals Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser

2015 ◽  
Vol 259 (3) ◽  
pp. 210-218 ◽  
Author(s):  
J. TRÄGÅRDH ◽  
G. ROBB ◽  
R. AMOR ◽  
W.B. AMOS ◽  
J. DEMPSTER ◽  
...  
Keyword(s):  
Excitation Spectrum ◽  
Fluorescent Dyes ◽  
Ti:Sapphire Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

A custom confocal and two-photon digital laser scanning microscope

2000 ◽  
Vol 278 (6) ◽  
pp. H2150-H2156 ◽  
Author(s):  
W. Gil Wier ◽  
C. William Balke ◽  
Jeffrey A. Michael ◽  
Joseph R. H. Mauban
Keyword(s):  
Laser Scanning ◽  
Photon Counting ◽  
Objective Lens ◽  
Scanning Microscope ◽  
Fluorescence Detector ◽  
Laser Scanning Microscope ◽  
Ti:Sapphire Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD (∼5 MHz). The confocal component is approximately nine times more efficient than our commercial confocal microscope (fluorophore fluo 4). Switching from one-photon to two-photon excitation mode (Ti:sapphire laser) is accomplished by moving a single mirror beneath the objective lens. The pulse from the Ti:sapphire laser is 109 fs in duration at the specimen plane, and average power is ∼5 mW. Two-photon excited fluorescence is detected by a fast photomultiplier tube. With a ×63 1.4 NA oil-immersion objective, the resolution of the confocal system is 0.25 μm laterally and 0.52 μm axially. For the two-photon system, the corresponding values are 0.28 and 0.82 μm. The system is advantageous when excitation intensity must be limited, when fluorescence is low, or when thick, scattering specimens are being studied (with two-photon excitation).

Two-photon excitation spectrum of perylene in solution

1990 ◽  
Vol 167 (1-2) ◽  
pp. 85-89 ◽  
Author(s):  
Jeong-A Yu ◽  
Daniel G. Nocera ◽  
George E. Leroi
Keyword(s):  
Excitation Spectrum ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Zeeman effect in the two-photon excitation spectrum of the C 2Π state of NO

1991 ◽  
Vol 156 (3) ◽  
pp. 509-522 ◽  
Author(s):  
S. Guizard ◽  
N. Shafizadeh ◽  
D. Chapoulard ◽  
M. Horani ◽  
D. Gauyacq
Keyword(s):  
Excitation Spectrum ◽  
Zeeman Effect ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

The two-photon excitation spectrum of fluorene near 6 K

1978 ◽  
Vol 30 (3) ◽  
pp. 343-351 ◽  
Author(s):  
A. Bree ◽  
M. Edelson ◽  
C. Taliani
Keyword(s):  
Excitation Spectrum ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two Photon Excitation Spectrum of a Twisted Quadruple Bond Metal−Metal Complex

1999 ◽  
Vol 121 (4) ◽  
pp. 868-869 ◽  
Author(s):  
Daniel S. Engebretson ◽  
Evan M. Graj ◽  
George E. Leroi ◽  
Daniel G. Nocera
Keyword(s):  
Metal Complex ◽  
Excitation Spectrum ◽  
Two Photon ◽  
Quadruple Bond ◽  
Photon Excitation ◽  
Metal Metal ◽  
Two Photon Excitation

Two‐photon excitation spectrum of benzene in the gas phase and the crystal

1974 ◽  
Vol 60 (1) ◽  
pp. 317-318 ◽  
Author(s):  
Robin M. Hochstrasser ◽  
J. E. Wessel ◽  
H. N. Sung
Keyword(s):  
Gas Phase ◽  
Excitation Spectrum ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Two-photon excitation spectrum of K2PtCl6

1982 ◽  
Vol 86 (5-6) ◽  
pp. 463-466 ◽  
Author(s):  
B.A. Kozikowski ◽  
T.A. Keiderling
Keyword(s):  
Excitation Spectrum ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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