Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy

2014 ◽  
Vol 256 (2) ◽  
pp. 117-125 ◽  
Author(s):  
T. ZIKMUND ◽  
L. KVASNICA ◽  
M. TÝČ ◽  
A. KŘÍŽOVÁ ◽  
J. ČOLLÁKOVÁ ◽  
...  
Keyword(s):  
Real Time ◽  
Digital Holographic Microscopy ◽  
Cell Behaviour ◽  
Quantitative Phase ◽  
Sequential Processing ◽  
Phase Images ◽  
Holographic Microscopy

scholarly journals Study on Quantitative Phase Imaging by Dual-Wavelength Digital Holography Microscopy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Lin Hu ◽  
Yunxiu Shui ◽  
Yaohui Dai ◽  
Haiyu Wu ◽  
Gang Zhu ◽  
...  
Keyword(s):  
Phase Distribution ◽  
Dual Wavelength ◽  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Quantitative Phase ◽  
Large Gradient ◽  
Phase Images ◽  
Surface Measurements ◽  
Holographic Microscopy ◽  
Error Of Measurement

A dual-wavelength digital holographic microscopy with premagnification is proposed to obtain the object surface measurements over the large gradient. The quantitative phase images of specimens are captured in high precision by the processing of filtering and phase compensation. The phase images are acquired without phase unwrapping, which is necessary in traditional digital holographic microscopy; thereby the proposed system can greatly increase the speed of reconstruction. The results of numerical simulation and optical experiments demonstrated that the reconstructed speed increased by 37.9 times, and the relative error of measurement is 4% compared with the traditional holographic microscopy system. It means that the proposed system can directly acquire the higher quality quantitative phase distribution for specimens.

scholarly journals Measuring the Cell-Induced Deformation of Collagen Matrix Detected With Digital Holographic Microscopy

2007 ◽  
Author(s):  
Kaveh Azartash ◽  
Enrico Gratton
Keyword(s):  
Mammalian Cells ◽  
Collagen Matrix ◽  
Spatial Arrangement ◽  
Digital Holographic Microscopy ◽  
Physical Aspect ◽  
Reconstruction Algorithms ◽  
Phase Images ◽  
Holographic Microscopy ◽  
Set Up ◽  
Charged Couple Device

A modified Mach-Zender set-up in reflection is applied to record and reconstruct holographic amplitude and phase images. A charged couple device (CCD) is used to record a hologram and numerical reconstruction algorithms are then applied to rebuild the hologram for obtaining both phase and amplitude information. One could also focus on multiple focal planes from a single hologram, similar to the focusing control of a conventional microscope. The morphology and behavior of mammalian cells is determined by an interaction between signals from the intracellular matrix and the cellular responses. It is important to note that the physical aspect of the extracellular matrix is as significant as the chemical nature of it. Specifically the stresses, mechanical forces, and the profile of the external environment have major effects on cell behavior. The mechanical and physical characteristics of a tissue are greatly dependent on a hierarchical spatial arrangement of its extra-cellular matrix components. A key player in the ECM is collagen which exhibits significant tensile strength on the cellular scale. Digital holographic microscopy (DHM) is applied to study the deformation of collage matrix in response to cell migration.

Experimental Study on Quantitative Phase Imaging by Digital Holographic Microscopy

2012 ◽  
Vol 39 (3) ◽  
pp. 0309002 ◽  
Author(s):  
马利红 Ma Lihong ◽  
王辉 Wang Hui ◽  
金洪震 Jin Hongzhen ◽  
李勇 Li Yong
Keyword(s):  
Experimental Study ◽  
Digital Holographic Microscopy ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Holographic Microscopy
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