scholarly journals The Class III Peroxidase gene OsPrx30 , Transcriptionally Modulated by the AT‐hook Protein OsATH1, Mediates Rice Bacterial Blight‐induced ROS Accumulation

2020 ◽  
Author(s):  
Hao Liu ◽  
Shuangyu Dong ◽  
Ming Li ◽  
Fengwei Gu ◽  
Guili Yang ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Class Iii ◽  
Peroxidase Gene ◽  
Rice Bacterial Blight ◽  
Ros Accumulation ◽  
Class Iii Peroxidase

Molecular cloning and characterization of a flower-specific class III peroxidase gene in G. Hirsutum

2007 ◽  
Vol 36 (3) ◽  
pp. 461-469 ◽  
Author(s):  
Dongyan Chen ◽  
Yezhang Ding ◽  
Wangzhen Guo ◽  
Tianzhen Zhang
Keyword(s):  
Molecular Cloning ◽  
Specific Class ◽  
Class Iii ◽  
Peroxidase Gene ◽  
Class Iii Peroxidase

Monitoring the transition from endodormancy to ecodormancy in almond through the analysis and expression of a specific class III peroxidase gene

2019 ◽  
Vol 15 (3) ◽  
Author(s):  
Angela S Prudencio ◽  
Pedro Díaz-Vivancos ◽  
Federico Dicenta ◽  
José Antonio Hernández ◽  
Pedro Martínez-Gómez
Keyword(s):  
Specific Class ◽  
Class Iii ◽  
Peroxidase Gene ◽  
Class Iii Peroxidase

scholarly journals A member of wheat class III peroxidase gene family, TaPRX-2A, enhanced the tolerance of salt stress

2020 ◽  
Author(s):  
Peisen Su ◽  
Jun Yan ◽  
Wen Li ◽  
Liang Wang ◽  
Jinxiao Zhao ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Abiotic Stresses ◽  
Evolutionary Model ◽  
Transgenic Wheat ◽  
Class Iii ◽  
Expression Levels ◽  
Peroxidase Gene ◽  
Stress Related Genes ◽  
Class Iii Peroxidase

Abstract Background: Salt and drought are the main abiotic stresses that restrict yield of crops. It is reported that peroxidases (PRXs) are involved in various abiotic stress responses. However, few wheat PRXs are characterized in the mechanism of abiotic stresses. Results: In this study, a novel wheat peroxidase (PRX) gene named TaPRX-2A, a member of wheat class III peroxidase gene family, was cloned and characterized in salt stress response. Based on the identification and evolutionary analysis of class III PRXs in 12 plants, we proposed an evolutionary model that TaPRX-2A might have experienced some exon fusion events during evolution. We also detected the positive selection of PRX domain in 13 PRXs involving our evolutionary model, and found 2 or 6 positively selected sites during TaPRX-2A evolution. The results of expression pattern showed that TaPRX-2A exhibited relatively higher expression levels in root tissue compared with that of leaf and stem tissues by using qRT-PCR. This TaPRX-2A was also induced by some stresses and hormone treatments including PEG6000, NaCl, hydrogen peroxide (H 2 O 2 ), salicylic acid (SA), methyljasmonic acid (MeJA) and abscisic acid (ABA). Transgenic wheat plants with overexpression of TaPRX-2A showed higher tolerance to salt stress than wild type (WT) plants. Confocal microscopy revealed that TaPRX-2A :eGFP was mainly localized in nuclei. The survival rate, relative water content and shoot length were higher in TaPRX-2A -overexpressing wheat than WT. However, root lengths were no significant difference between transgenic wheat and WT. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were enhanced in TaPRX-2A -overexpressing wheat than WT, resulting in the reduction of reactive oxygen species (ROS) accumulation and malondialdehyde (MDA) content. We also measured the expression levels of downstream stress-related genes ( RD22 , TLP4 , ABAI , GLP4 , GST22 , FeSOD, CuSOD, and CAT ). The results showed that RD22 , TLP4 , ABAI , GST22 , FeSOD, and CAT exhibited higher expression in TaPRX-2A -overexpressing lines than in WT under salt stress. Conclusions: The results show that TaPRX-2A plays a positive factor in response to salt stress by scavenging ROS and regulating stress-related genes.

scholarly journals Impaired Cuticle Functionality and Robust Resistance to Botrytis cinerea in Arabidopsis thaliana Plants With Altered Homogalacturonan Integrity Are Dependent on the Class III Peroxidase AtPRX71

2021 ◽  
Vol 12 ◽  
Author(s):  
Riccardo Lorrai ◽  
Fedra Francocci ◽  
Kay Gully ◽  
Helle J. Martens ◽  
Giulia De Lorenzo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Botrytis Cinerea ◽  
Primary Cell Wall ◽  
Loss Of Function ◽  
Class Iii ◽  
Ros Accumulation ◽  
Receptor Like Kinase ◽  
Cuticle Ultrastructure ◽  
Class Iii Peroxidase

Pectin is a major cell wall component that plays important roles in plant development and response to environmental stresses. Arabidopsis thaliana plants expressing a fungal polygalacturonase (PG plants) that degrades homogalacturonan (HG), a major pectin component, as well as loss-of-function mutants for QUASIMODO2 (QUA2), encoding a putative pectin methyltransferase important for HG biosynthesis, show accumulation of reactive oxygen species (ROS), reduced growth and almost complete resistance to the fungal pathogen Botrytis cinerea. Both PG and qua2 plants show increased expression of the class III peroxidase AtPRX71 that contributes to their elevated ROS levels and reduced growth. In this work, we show that leaves of PG and qua2 plants display greatly increased cuticle permeability. Both increased cuticle permeability and resistance to B. cinerea in qua2 are suppressed by loss of AtPRX71. Increased cuticle permeability in qua2, rather than on defects in cuticle ultrastructure or cutin composition, appears to be dependent on reduced epidermal cell adhesion, which is exacerbated by AtPRX71, and is suppressed by the esmeralda1 mutation, which also reverts the adhesion defect and the resistant phenotype. Increased cuticle permeability, accumulation of ROS, and resistance to B. cinerea are also observed in mutants lacking a functional FERONIA, a receptor-like kinase thought to monitor pectin integrity. In contrast, mutants with defects in other structural components of primary cell wall do not have a defective cuticle and are normally susceptible to the fungus. Our results suggest that disrupted cuticle integrity, mediated by peroxidase-dependent ROS accumulation, plays a major role in the robust resistance to B. cinerea of plants with altered HG integrity.

scholarly journals TROUBLESHOOTING OF CLASS III PEROXIDASE GENE FROM THE MOSS DICRANUM SCOPARIUM

ÈKOBIOTEH ◽  
2020 ◽  
Vol 3 (2) ◽  
pp. 174-180
Author(s):  
A.O. Onele ◽  
◽  
A.V. Chasov ◽  
F.V. Minibayeva ◽  
◽  
...  
Keyword(s):  
Class Iii ◽  
Peroxidase Gene ◽  
Dicranum Scoparium ◽  
Class Iii Peroxidase

Cold-Responsive Regulation of a Flower-Preferential Class III Peroxidase Gene, OsPOX1, in Rice (Oryza sativa L.)

2011 ◽  
Vol 55 (2) ◽  
pp. 123-131 ◽  
Author(s):  
Sung-Hyun Kim ◽  
Hong-Seok Choi ◽  
Young-Chan Cho ◽  
Seong-Ryong Kim
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Class Iii ◽  
Peroxidase Gene ◽  
Responsive Regulation ◽  
Class Iii Peroxidase
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