scholarly journals Molecular cloning and functional analysis of the FLOWERING LOCUS T (FT ) homolog GhFT1 from Gossypium hirsutum

2015 ◽  
Vol 57 (6) ◽  
pp. 522-533 ◽  
Author(s):  
Danli Guo ◽  
Chao Li ◽  
Rui Dong ◽  
Xiaobo Li ◽  
Xiangwen Xiao ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Gossypium Hirsutum ◽  
Molecular Cloning ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals Molecular Cloning and Functional Analysis of Three FLOWERING LOCUS T (FT) Homologous Genes from Chinese Cymbidium

2012 ◽  
Vol 13 (9) ◽  
pp. 11385-11398 ◽  
Author(s):  
Weiting Huang ◽  
Zhongming Fang ◽  
Songjun Zeng ◽  
Jianxia Zhang ◽  
Kunlin Wu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Cloning ◽  
Flowering Locus T ◽  
Homologous Genes ◽  
Flowering Locus

Isolation and functional analysis of FLOWERING LOCUS T orthologous gene from Vanda hybrid

2019 ◽  
Vol 28 (4) ◽  
pp. 374-381
Author(s):  
Kanokwan Panjama ◽  
Eriko Suzuki ◽  
Masahiro Otani ◽  
Masaru Nakano ◽  
Norikuni Ohtake ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Orthologous Gene ◽  
Flowering Locus T ◽  
Flowering Locus

scholarly journals Characterization and Activity Analyses of the FLOWERING LOCUS T Promoter in Gossypium Hirsutum

2019 ◽  
Vol 20 (19) ◽  
pp. 4769 ◽  
Author(s):  
Na Sang ◽  
Darun Cai ◽  
Chao Li ◽  
Yuqiang Sun ◽  
Xianzhong Huang
Keyword(s):  
Gossypium Hirsutum ◽  
Regulatory Elements ◽  
Proximal Promoter ◽  
Activity Levels ◽  
Loss Of Function ◽  
Flowering Locus T ◽  
Promoter Regions ◽  
Core Motif ◽  
Late Flowering ◽  
Flowering Locus

Flowering transition is a crucial development process in cotton (Gossypium hirsutum L.), and the flowering time is closely correlated with the timing of FLOWERING LOCUS T (FT) expression. However, the mechanism underlying the coordination of various cis-regulatory elements in the FT promoter of cotton has not been determined. In this study, a 5.9-kb promoter of FT was identified from cotton. A bioinformatics analysis showed that multiple insertion–deletion sites existed in the 5.9-kb promoter. Different expression levels of a reporter gene, and the induction by sequential deletions in GhFT promoter, demonstrated that 1.8-kb of the GhFT promoter was stronger than 4.2-, 4.8-, and 5.9-kb promoter fragments. The binding sites of the CONSTANS (CO) and NUCLEAR FACTOR Y transcription factors were located within the 1.0-kb sequence upstream of the FT transcription start site. A large number of repeat segments were identified in proximal promoter regions (−1.1 to −1.4 kb). A complementation analysis of deletion constructs between 1.0 and 1.8 kb of G. hirsutum, Gossypium arboretum, and Gossypium raimondii FT promoters revealed that the 1.0-kb fragment significantly rescued the late-flowering phenotype of the Arabidopsis FT loss-of-function mutant ft-10, whereas the 1.8-kb promoter only slightly rescued the late-flowering phenotype. Furthermore, the conserved CORE motif in the cotton FT promoter is an atypical TGTG(N2-3)ATG, but the number of arbitrary bases between TGTG and ATG is uncertain. Thus, the proximal FT promoter region might play an important role affecting the activity levels of FT promoters in cotton flowering.

scholarly journals Molecular characterization and functional analysis of a Flowering locus T homolog gene from a Phalaenopsis orchid

2014 ◽  
Vol 13 (3) ◽  
pp. 5982-5994 ◽  
Author(s):  
D.M. Li ◽  
F.B. Lǚ ◽  
G.F. Zhu ◽  
Y.B. Sun ◽  
H.L. Liu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Molecular Characterization ◽  
Flowering Locus T ◽  
Flowering Locus
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