Multiplex polymerase chain reaction detection of selected bacterial species from symptomatic and asymptomatic non-vital teeth with primary endodontic infections

2018 ◽  
Vol 9 (2) ◽  
pp. e12312 ◽  
Author(s):  
Smita Pattanshetty ◽  
Vijayalakshmi S. Kotrashetti ◽  
Kishore Bhat ◽  
Ramakant S. Nayak ◽  
Pradeep Somannavar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Bacterial Species ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Endodontic Infections ◽  
Polymerase Chain

scholarly journals Simplified Method of Detection of Dialister invisus and Olsenella uli in Oral Cavity Samples by Polymerase Chain Reaction

2017 ◽  
Vol 8 (1-2) ◽  
pp. 47-52 ◽  
Author(s):  
Manohar S. Kugaji ◽  
Kishore G. Bhat ◽  
Vinayak M. Joshi ◽  
Madhu Pujar ◽  
Pratik T. Mavani
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Species ◽  
Universal Primers ◽  
Sample Collection ◽  
Chain Reaction ◽  
Infection Group ◽  
Endodontic Infection ◽  
Predominant Component ◽  
Endodontic Infections ◽  
Polymerase Chain

Aims and Objectives: The oral microbial flora is highly complex and diverse with obligate anaerobic bacteria as the predominant component. Most of these are not yet cultivated/difficult to cultivate due to technical limitations. In this study, we aim to detect two novel oral bacterial species Dialister invisus and Olsenella uli by simplified and economical procedure of polymerase chain reaction (PCR) and study their association with primary and persistent endodontic infections. Material and Methods: The study involved 60 patients that included 30 patients of primary endodontic infections and 30 with persistent endodontic infections. The sample collection from the root canal was performed by universally accepted protocol by using sterile paper points. The deoxyribonucleic acid (DNA) extraction was done, followed by PCR with species specific primers. We made several changes to the protocol mentioned by original authors. We adopted a one-step protocol for amplification of bacterial DNA, omitting the 16SrDNA amplification step with universal primers. Results: It was seen that 7 (23.3 %) samples in primary endodontic infection group and 24 (80 %) samples in persistent endodontic infection group were positive for D. invisus. On the other hand, 11 (36.6 %) patients of primary endodontic infection showed positivity for O. uli in comparison to 9 (30 %) of persistent endodontic infection. Conclusion: The results from the present study showed efficient amplification of both O. uli and D. invisus in a single-step PCR. Hence, we conclude that the modified protocol used here with taq polymerase enzyme offers a faster and cheaper alternative to nested PCR without compromising the quality of amplification process.

scholarly journals Detection of selected bacterial species in intraoral sites of patients with chronic periodontitis using multiplex polymerase chain reaction

2010 ◽  
Vol 18 (4) ◽  
pp. 426-431 ◽  
Author(s):  
Cyntia Rodrigues de Araújo Estrela ◽  
Fabiana Cristina Pimenta ◽  
Ana Helena Gonçalves de Alencar ◽  
Luis Fernando Naldi Ruiz ◽  
Carlos Estrela
Keyword(s):  
Polymerase Chain Reaction ◽  
Chronic Periodontitis ◽  
Multiplex Polymerase Chain Reaction ◽  
Bacterial Species ◽  
Chain Reaction ◽  
Polymerase Chain

Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia*

2008 ◽  
Vol 36 (5) ◽  
pp. 1487-1492 ◽  
Author(s):  
Richard F. Louie ◽  
Zuping Tang ◽  
Timothy E. Albertson ◽  
Stuart Cohen ◽  
Nam K. Tran ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Detection Enhancement ◽  
Polymerase Chain
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