Post-antifungal effect and adhesion to buccal epithelial cells of oral Candida dubliniensis isolates subsequent to limited exposure to amphotericin B, ketoconazole and fluconazole

2014 ◽  
Vol 6 (3) ◽  
pp. 186-192 ◽  
Author(s):  
Arjuna N. B. Ellepola ◽  
Rachel Chandy ◽  
Zia U. Khan
Keyword(s):  
Epithelial Cells ◽  
Amphotericin B ◽  
Candida Dubliniensis ◽  
Antifungal Effect ◽  
Buccal Epithelial Cells ◽  
Oral Candida

scholarly journals In vitro Post-Antifungal Effect of Posaconazole and Its Impact on Adhesion-Related Traits and Hemolysin Production of Oral Candida dubliniensis Isolates

2019 ◽  
Vol 28 (6) ◽  
pp. 552-558
Author(s):  
Arjuna Nishantha Bandara Ellepola ◽  
Ranil Samantha Dassanayake ◽  
Ziauddin Khan
Keyword(s):  
Oral Candidiasis ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Candida Dubliniensis ◽  
In Vitro Assays ◽  
Drug Induced ◽  
Antifungal Effect ◽  
Buccal Epithelial Cells ◽  
Oral Candida

Objective: Candidal adherence to denture acrylic surfaces (DAS) and oral buccal epithelial cells (BEC), formation of candidal germ tubes (GT), candidal cell surface hydrophobicity (CSH), and hemolysin production are important pathogenic traits of Candida. The antifungal drug-induced post-antifungal effect (PAFE) also impacts the virulence of Candida. Candida dubliniensis isolates are associated with the causation of oral candidiasis which could be managed with posaconazole. Thus far there is no evidence on posaconazole-induced PAFE and its impact on adhesion-related attributes and production of hemolysin by C. dubliniensis isolates. Hence, the PAFE, adhesion to DAS and BEC, formation of GT, CSH, and hemolysin production of 20 oral C. dubliniensis isolates after brief exposure to posaconazole was ascertained. Materials and Methods: The PAFE, adherence to DAS and BEC, formation of GT, candidal CSH, and hemolysin production were investigated by hitherto described in vitro assays. Results: The mean PAFE (h) induced by posaconazole on C. dubliniensis isolates was 1.66. Exposure to posaconazole suppressed the ability of C. dubliniensis to adhere to DAS, BEC, formation of candidal GT, candidal CSH and to produce hemolysin by a reduction of 44, 33, 34, 36, and 15% (p < 0.005 to p < 0.001), respectively. Conclusion: Exposure of C. dubliniensis isolates to posaconazole for a brief period induced an antimycotic impact by subduing its growth in addition to suppressing pathogenic adherence-associated attributes, as well as production of hemolysin.

scholarly journals Impact of Brief Exposure to Drugs with Antifungal Properties on the Susceptibility of Oral Candida dubliniensis Isolates to Lysozyme and Lactoferrin

2018 ◽  
Vol 27 (6) ◽  
pp. 523-530 ◽  
Author(s):  
Arjuna Nishantha B. Ellepola ◽  
Ranil Samantha Dassanayake ◽  
Ziauddin Khan
Keyword(s):  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Oral Candidiasis ◽  
Therapeutic Agents ◽  
Candida Dubliniensis ◽  
Antifungal Effect ◽  
Candida Infections ◽  
Oral Candida

Objective: Lysozyme and lactoferrin have anti-candidal activity. Candida dubliniensis is associated with oral candidiasis. Candida infections are managed with nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine. Candida species undergo a brief exposure to therapeutic agents in the mouth. There is no data on the influence of limited exposure to antimycotics on the sensitivity of C. dubliniensis to lactoferrin and lysozyme. Hence, this study observed the changes in the sensitivity of C. dubliniensis to anti-candidal action of lactoferrin and lysozyme after transitory exposure to sub-lethal concentrations of antifungals. Materials and Methods: After determination of the minimum inhibitory concentration (MIC), 20 C. dubliniensis isolates were exposed to twice the concentration of MIC of nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine for 1 h. Drugs were removed by dilution and thereafter the susceptibility of these isolates to lysozyme and lactoferrin was determined by colony-forming unit quantification assay. Results: Exposure of C. dubliniensis to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine resulted in an increase in susceptibility to lysozyme by 9.45, 30.82, 30.04, 50.64, 55.60, and 50.18%, respectively (p < 0.05 to p < 0.001). Exposure of C. dubliniensis to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine resulted in an increase in susceptibility to lactoferrin by 13.54, 16.43, 17.58, 19.60, 21.32, and 18.73, respectively (p < 0.05 to p < 0.001). Conclusion: Brief exposure to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine enhances the antifungal effect of lysozyme and lactoferrin on C. dubliniensis isolates in vitro.

scholarly journals Impact of brief and sequential exposure to nystatin, amphotericin B, ketoconazole, and fluconazole in modulating adhesion traits of oral Candida dubliniensis isolates

2014 ◽  
Vol 7 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Arjuna N.B. Ellepola ◽  
Bobby K. Joseph ◽  
Lakshman P. Samaranayake ◽  
H.M.H.N. Bandara ◽  
Zia U. Khan
Keyword(s):  
Amphotericin B ◽  
Candida Dubliniensis ◽  
Oral Candida

scholarly journals Comparative adherence of Candida albicans and Candida dubliniensis to human buccal epithelial cells and extracellular matrix proteins

2014 ◽  
Vol 52 (3) ◽  
pp. 254-263 ◽  
Author(s):  
Rachael P. C. Jordan ◽  
David W. Williams ◽  
Gary P. Moran ◽  
David C. Coleman ◽  
Derek J. Sullivan
Keyword(s):  
Extracellular Matrix ◽  
Candida Albicans ◽  
Epithelial Cells ◽  
Extracellular Matrix Proteins ◽  
Candida Dubliniensis ◽  
Matrix Proteins ◽  
Buccal Epithelial Cells
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