Effect of human whole saliva on the in vitro adhesion of Candida albicans to a denture base acrylic resin: a focus on collection and preparation of saliva samples

2012 ◽  
Vol 4 (4) ◽  
pp. 225-228 ◽  
Author(s):  
Camila A. Zamperini ◽  
Patricia C. S. Schiavinato ◽  
Ana C. Pavarina ◽  
Eunice T. Giampaolo ◽  
Carlos E. Vergani ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin ◽  
Denture Base ◽  
Whole Saliva ◽  
In Vitro Adhesion

scholarly journals Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro

Materials ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 221
Author(s):  
Avi Meirowitz ◽  
Arkadi Rahmanov ◽  
Eti Shlomo ◽  
Helena Zelikman ◽  
Eran Dolev ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Candida Albicans ◽  
3D Printing ◽  
Oral Candidiasis ◽  
Acrylic Resin ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Heat Curing ◽  
Vitro Effect

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

scholarly journals A Comparative Evaluation of the Dimensional Accuracy of Heat Polymerized Acrylic Resin Denture Base Clamped by the Conventional Method and by New-press Technique and Cured by Long Curing Cycle: An in vitro Study

2012 ◽  
Vol 13 (6) ◽  
pp. 842-849
Author(s):  
T Shankar ◽  
N Venugopal Rao ◽  
Snigdha Gowd ◽  
Syed Tauqheer Ahmed ◽  
V Vinod ◽  
...  
Keyword(s):  
Conventional Method ◽  
Comparative Evaluation ◽  
Dimensional Accuracy ◽  
Acrylic Resin ◽  
Reference Points ◽  
Control Group ◽  
Denture Base ◽  
Group A ◽  
Curing Cycle

ABSTRACT Aim The aim of this study is to evaluate the dimensional accuracy of heat polymerized acrylic resin denture base clamped by the conventional method and by new-press technique and cured by long curing cycle. Materials and methods In this study, a total of 60 standardized maxillary record bases were fabricated with seven reference points as follows: • Point A Incisive papilla, • Point B and C Canine region on either side • Point E and G Midpoint of tuberosities on either side • Point F Midpoint of the line joining the two tuberosities • Point D Midpoint between the line joining A and F Group A Ten maxillary record bases were fabricated by conventional clamping method and cured by long curing cycle. Group A’ Ten maxillary record bases were fabricated by New Press or RS tension clamping method and cured by long curing cycle. The distances between the reference points, i.e. A-B, A-C, A-D, D-F, B-E, C-G, E-F, F-G, B-D, D-G, C-D, D-E of all three thermoplastic denture base plates were measured and recorded with the help of travelling microscope and were used for comparison with the measured and recorded readings of processed acrylic denture bases. The data obtained was analyzed by using the one-way analysis of variance and HSD Multiple Comparison Test. Results The overall results of the study indicate that among all the denture bases cured by the two clamping systems and the long curing cycle, group A‘ were the most dimensionally stable, followed by control group A. Conclusion The study concluded that the denture bases fabricated by the New Press method using the long curing cycle would produce the most dimensionally stable denture bases. How to cite this article Shankar T, Gowd S, Ahmed ST, Vinod V, Goud MV, Rao NV. A Comparative Evaluation of the Dimensional Accuracy of Heat Polymerized Acrylic Resin Denture Base Clamped by the Conventional Method and by New-press Technique and Cured by Long Curing Cycle: An in vitro Study. J Contemp Dent Pract 2012;13(6):842-849.

In Vitro Adherence of Candida albicans to Acrylic Resin with Different Surface Status

2012 ◽  
pp. 145-146
Author(s):  
Taro Nomura ◽  
Tetsuya Suzuki ◽  
Junichi Furuya ◽  
Yu Shimoyama ◽  
Minoru Sasaki ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin

scholarly journals Influence of Acrylic Resin Denture Base Soaking Length in Siwak Extract Solution (Salvadora persica) on the Growth of Candida albicans

2018 ◽  
Vol 18 (1) ◽  
pp. 1-6
Author(s):  
Irene Edith Rieuwpassa ◽  
Iman Sudjarwo ◽  
Sumintarti S ◽  
Susilowati Mudjari ◽  
Harun Achmad ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Acrylic Resin ◽  
Salvadora Persica ◽  
Denture Base ◽  
Extract Solution

scholarly journals Effect of Denture Base Reinforcement Using Light Cured E- Glass Fibers on the Level of Salivary Immunoglobulin A

2018 ◽  
Vol 6 (11) ◽  
pp. 2168-2172
Author(s):  
Shady M. El Naggar ◽  
Mohamed I. Seif El Nasr ◽  
Hassan M. Sakr ◽  
Sherihan M. Eissa ◽  
Asmaa N. Elboraey ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Acrylic Resin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Complete Dentures ◽  
Denture Base ◽  
Mean Values ◽  
Glass Fibres ◽  
Significant Difference ◽  
Salivary Immunoglobulin A

BACKGROUND: A gap still exists between in vitro and clinical studies concerning the biocompatibility of the material in the oral environment and their potential to cause immunological undesirable side effects. The uses of glass fibres to improve the mechanical properties of acrylic resin denture base polymers are well documented in vitro. AIM: The present study aimed to evaluate the effect of denture base reinforcement using light-cured E- glass fibres mesh on the level of salivary immunoglobulin A (S-IgA) in patients wearing complete dentures. MATERIAL AND METHODS: Fourteen completely edentulous patients, in need of complete dentures, participated in the study. The patients were divided into two groups (n = 7) according to the treatment protocol. In the first group, patients received conventional heat-cured acrylic resin dentures. In the second group, the mandibular dentures were reinforced using light cured resin impregnated E glass fibres mesh. In both groups, salivary samples were collected using passive drool technique. The level IgA was assessed by enzyme-linked immunosorbent assay (ELISA) technique at different time intervals. Statistical analysis was carried out using one-way ANOVA followed by Tukey`s post-hoc test and independent t-test. The significant level was set at P ≤ 0.05. RESULTS: Acrylic resin dentures and reinforced ones demonstrated an increase in the mean values of IgA level at the end of the follow-up intervals. And this increase was statistically significant (P ≤ 0.05). Although, the reinforced dentures revealed higher mean values, there was no statistically significant difference between the two groups (P > 0.05) CONCLUSIONS: Within the limitations of the present study, the following could be concluded: (1) the insertion of complete dentures induced changes in the level of IgA; and (2) denture base reinforcement using light cured resin impregnated E-glass fibres mesh had a similar effect to that of heat cured acrylic resin on the level of IgA.

In vitro adhesion of Candida species to denture base materials

Mycoses ◽  
2006 ◽  
Vol 49 (2) ◽  
pp. 80-84 ◽  
Author(s):  
X. Y. He ◽  
J. H. Meurman ◽  
K. Kari ◽  
R. Rautemaa ◽  
L. P. Samaranayake
Keyword(s):  
Candida Species ◽  
Denture Base ◽  
Base Materials ◽  
In Vitro Adhesion
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