Acetylcholine induces fibrogenic effects via M2/M3 acetylcholine receptors in non-alcoholic steatohepatitis and in primary human hepatic stellate cells

2016 ◽  
Vol 31 (2) ◽  
pp. 475-483 ◽  
Author(s):  
Maelle L Morgan ◽  
Barbara Sigala ◽  
Junpei Soeda ◽  
Paul Cordero ◽  
Vi Nguyen ◽  
...  
Keyword(s):  
Hepatic Stellate Cells ◽  
Acetylcholine Receptors ◽  
Stellate Cells ◽  
Alcoholic Steatohepatitis

Chemokine (CC-motif) receptor-like 2 mRNA is expressed in hepatic stellate cells and is positively associated with characteristics of non-alcoholic steatohepatitis in mice and men

2017 ◽  
Vol 103 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Sebastian Zimny ◽  
Rebekka Pohl ◽  
Lisa Rein-Fischboeck ◽  
Elisabeth M. Haberl ◽  
Sabrina Krautbauer ◽  
...  
Keyword(s):  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Alcoholic Steatohepatitis

scholarly journals Amphiregulin activates human hepatic stellate cells and is upregulated in non alcoholic steatohepatitis

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Chad McKee ◽  
Barbara Sigala ◽  
Junpei Soeda ◽  
Angelina Mouralidarane ◽  
Maelle Morgan ◽  
...  
Keyword(s):  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Alcoholic Steatohepatitis

scholarly journals Heterogeneity of hepatic stellate cells in a mouse model of non‐alcoholic steatohepatitis (NASH)

Hepatology ◽  
2021 ◽  
Author(s):  
Sara Brin Rosenthal ◽  
Xiao Liu ◽  
Souradipta Ganguly ◽  
Debanjan Dhar ◽  
Martina P. Pasillas ◽  
...  
Keyword(s):  
Mouse Model ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Alcoholic Steatohepatitis

scholarly journals Chemerin Overexpression in the Liver Protects against Inflammation in Experimental Non-Alcoholic Steatohepatitis

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 132
Author(s):  
Rebekka Pohl ◽  
Susanne Feder ◽  
Elisabeth M. Haberl ◽  
Lisa Rein-Fischboeck ◽  
Thomas S. Weiss ◽  
...  
Keyword(s):  
Hepatic Stellate Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Stellate Cells ◽  
Mrna Levels ◽  
Peripheral Blood Mononuclear ◽  
Alcoholic Steatohepatitis ◽  
Huh7 Cells ◽  
Chemokine Ligand ◽  
Underlying Mechanisms

Non-alcoholic steatohepatitis (NASH) is marked by macrophage infiltration and inflammation. Chemerin is a chemoattractant protein and is abundant in hepatocytes. The aim of this study was to gain insight into the role of hepatocyte-produced prochemerin in NASH. Therefore, mice were infected with adeno-associated virus 8 to direct hepatic overexpression of prochemerin in a methionine–choline deficient dietary model of NASH. At the end of the study, hepatic and serum chemerin were higher in the chemerin-expressing mice. These animals had less hepatic oxidative stress, F4/80 and CC-chemokine ligand 2 (CCL2) protein, and mRNA levels of inflammatory genes than the respective control animals. In order to identify the underlying mechanisms, prochemerin was expressed in hepatocytes and the hepatic stellate cells, LX-2. Here, chemerin had no effect on cell viability, production of inflammatory, or pro-fibrotic factors. Notably, cultivation of human peripheral blood mononuclear cells (PBMCs) in the supernatant of Huh7 cells overexpressing chemerin reduced CCL2, interleukin-6, and osteopontin levels in cell media. CCL2 was also low in RAW264.7 cells exposed to Hepa1–6 cell produced chemerin. In summary, the current study showed that prochemerin overexpression had little effect on hepatocytes and hepatic stellate cells. Of note, hepatocyte-produced chemerin deactivated PBMCs and protected against inflammation in experimental NASH.

scholarly journals Microengineered cultures containing human hepatic stellate cells and hepatocytes for drug development

2017 ◽  
Vol 9 (8) ◽  
pp. 662-677 ◽  
Author(s):  
Matthew D. Davidson ◽  
David A. Kukla ◽  
Salman R. Khetani
Keyword(s):  
Drug Development ◽  
High Throughput ◽  
Drug Screening ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Human Hepatocytes ◽  
Alcoholic Steatohepatitis

Micropatterned tri-cultures (MPTCs) containing human hepatocytes, hepatic stellate cells, and fibroblasts in a high-throughput format are used to mimic aspects of non-alcoholic steatohepatitis (NASH) for drug screening.

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