Side population cell fractions from hepatocellular carcinoma cell lines increased with tumor dedifferentiation, but lack characteristic features of cancer stem cells

2014 ◽  
Vol 29 (5) ◽  
pp. 1092-1101 ◽  
Author(s):  
Masamichi Nakayama ◽  
Sachiko Ogasawara ◽  
Jun Akiba ◽  
Kosuke Ueda ◽  
Keiko Koura ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Carcinoma Cell ◽  
Side Population ◽  
Side Population Cell ◽  
Carcinoma Cell Lines ◽  
Hepatocellular Carcinoma Cell ◽  
Characteristic Features ◽  
Cell Fractions

scholarly journals Identification of cancer stem cells from hepatocellular carcinoma cell lines and their related microRNAs

2013 ◽  
Vol 30 (5) ◽  
pp. 2056-2062 ◽  
Author(s):  
YANGMEI XU ◽  
YUNQING XIE ◽  
XIANGRU WANG ◽  
XUEFANG CHEN ◽  
QINGYIN LIU ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Hepatocellular Carcinoma Cell

scholarly journals Increased Oxidative Phosphorylation Is Required for Stemness Maintenance in Liver Cancer Stem Cells from Hepatocellular Carcinoma Cell Line HCCLM3 Cells

2020 ◽  
Vol 21 (15) ◽  
pp. 5276 ◽  
Author(s):  
Ge Liu ◽  
Qing Luo ◽  
Hong Li ◽  
Qiuping Liu ◽  
Yang Ju ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Energy Metabolism ◽  
Cancer Stem Cells ◽  
Liver Cancer ◽  
Oxidative Phosphorylation ◽  
Carcinoma Cell ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell ◽  
The Relationship

Cancer stem cells (CSCs) are considered to be the main cause of tumor recurrence, metastasis, and an unfavorable prognosis. Energy metabolism is closely associated with cell stemness. However, how the stemness of liver cancer stem cells (LCSCs) is regulated by metabolic/oxidative stress remains poorly understood. In this study, we compare the metabolic differences between LCSCs and the hepatocellular carcinoma cell line HCCLM3, and explore the relationship between metabolism and LCSC stemness. We found that LCSCs from the hepatocellular carcinoma cell HCCLM3 exhibited more robust glucose metabolism than HCCLM3, including glycolysis, oxidative phosphorylation (OXPHOS), and pyruvate produced by glycolysis entering mitochondria for OXPHOS. Moreover, 2-deoxy-D-glucose (2-DG) enhanced the LCSC stemness by upregulating OXPHOS. In contrast, Mdivi-1 reduced the levels of OXPHOS and weakened the stemness by inhibiting mitochondrial fission. Together, our findings clarify the relationship between energy metabolism and LCSC stemness and may provide theoretical guidance and potential therapeutic approaches for liver cancer.

scholarly journals A robust culture method for maintaining tumorigenic cancer stem cells in the hepatocellular carcinoma cell line Li‐7

2019 ◽  
Vol 110 (5) ◽  
pp. 1644-1652
Author(s):  
Yukako Sato ◽  
Takeshi Yamada ◽  
Takashi Hiroyama ◽  
Kazuhiro Sudo ◽  
Naoyuki Hasegawa ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Culture Method ◽  
Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell

scholarly journals CD44 standard isoform is involved in maintenance of cancer stem cells of a hepatocellular carcinoma cell line

2019 ◽  
Vol 8 (2) ◽  
pp. 773-782 ◽  
Author(s):  
Ryoma Asai ◽  
Hiroyuki Tsuchiya ◽  
Masataka Amisaki ◽  
Kazuki Makimoto ◽  
Ai Takenaga ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell Line ◽  
Hepatocellular Carcinoma Cell
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