Contamination of freshly harvested Bambara groundnut ( Vigna subterranea ) seed from Mpumalanga, South Africa, with mycotoxigenic fungi

2020 ◽  
Vol 40 (6) ◽  
Author(s):  
Margot Otto ◽  
Beulah Pretorius ◽  
Quenton Kritzinger ◽  
Hettie Schönfeldt
2017 ◽  
Vol 32 (2) ◽  
pp. 208-224 ◽  
Author(s):  
Amara Evangeline Unigwe ◽  
Enrico Doria ◽  
Patrick Adebola ◽  
Abe. S Gerrano ◽  
Michael Pillay

2016 ◽  
Vol 8 (11) ◽  
pp. 69 ◽  
Author(s):  
Amara Evangeline Unigwe ◽  
Abe Shegro Gerrano ◽  
Patrick Adebola ◽  
Michael Pillay

<p>Bambara groundnut (<em>Vigna subterranea </em>L. Verdc) is an underutilized crop in the African continent. It is a drought tolerant crop and fixes atmospheric nitrogen. Bambara groundnut is primarily grown for the protein content of its seeds and is mainly produced by small scale farmers at subsistence level. The objective of the study was to assess the morphological variation of landraces of bambara groundnut in South Africa. Thirty accessions of bambara groundnut were evaluated for their variability in agronomic and morphological traits. The field experiment was conducted at ARC-VOPI in Roodeplaat research farm during the 2014/2015 summer cropping season. The field trial was arranged as a complete randomized block design with three replications. Eighteen quantitative traits were recorded to estimate the level of genetic variability among accessions. The analysis of variance revealed significant differences among the phenotypic traits evaluated. The UPGMA cluster analysis based on the quantitative traits produced four distinct groups of genotypes and a singleton. Genotypes SB11-1A, SB19-1A, SB12-3B and Bambara-12 were found to possess good vegetative characters and are recommended for use as suitable parents when breeding cultivars for fodder production. Desirable yield and yield-related traits were identified in B7-1, SB4-4C, SB19-1A, Bambara-12 and SB16-5A and are recommended as suitable parental lines for bambara groundnut grain production improvement. The phenotypic characters therefore provide a useful measure of genetic variability among bambara genotypes and will enable the identification of potential parental materials for future breeding programs in South Africa.</p>


Heliyon ◽  
2018 ◽  
Vol 4 (9) ◽  
pp. e00833 ◽  
Author(s):  
Taahir Harris ◽  
Victoria Jideani ◽  
Marilize Le Roes-Hill

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adré Minnaar-Ontong ◽  
Abe S. Gerrano ◽  
Maryke T. Labuschagne

AbstractWith its drought tolerant and protein-rich properties, Bambara groundnut [Vigna subterranea (L.) Verdc.], an indigenous African legume crop can contribute immensely to food security. This miracle crop is used as food and for the enhancement of soil fertility in South Africa. Knowledge on the genetic diversity and structure among the Bambara groundnut landraces can pave the way for the effective use and cultivation of this crop in southern Africa, especially South Africa. The aim of this study was to assess the genetic diversity and structure among Bambara groundnut landraces collected across South Africa and compared to a limited number of accessions from southern Africa using SSR markers. Seventy-eight Bambara groundnut accessions were genotyped using 19 Bambara specific SSR markers. SSR loci explored in this study, were all polymorphic. A total of 127 alleles were detected with a mean of 6.7 alleles per locus. Allele diversity and frequency among genotypes varied from 0.21 to 0.85 with an average of 0.62 per locus. Genetic variation as described by the analysis of molecular variance indicated higher genetic diversity (92%) within landraces than between (8%) different landraces. Population structure analysis showed that three subpopulations existed, and most of the South African accessions were restricted to one subpopulation, indicating that Bambara landraces has the ability to form unique haplotypes in different environments. Information harnessed in this study is helpful for further use in breeding programs for crop improvement.


2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn


Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.


PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0204817 ◽  
Author(s):  
Juliet Mubaiwa ◽  
Vincenzo Fogliano ◽  
Cathrine Chidewe ◽  
Evert Jan Bakker ◽  
Anita R. Linnemann

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Siti Fatimah ◽  
ARIFFIN ARIFFIN ◽  
ARDIARINI NOER RAHMI ◽  
KUSWANTO KUSWANTO

Abstract. Fatimah S, Ariffin, Rahmi AN, Kuswanto. 2020. Tolerance and determinants of drought character descriptors of the Madurese landrace bambara groundnut (Vigna subterranea). Biodiversitas 21: 3108-3116. Bambara groundnut (Vigna subterranea L. Verdc.) is legume of African origin overlooked in Indonesia. It has underdeveloped in Indonesia; for example, in East Java, it is only cultivated in Gresik, Lamongan, and Bangkalan of Madura. This plant can potentially be developed in dry lands, such as Madura as it has the ability to grow and develop well in a dry environment with low nutrient level. At present, there are sparse researches on the selection and determination of the drought-tolerant character descriptors of bambara groundnut in Indonesia. The present study used the expected lines of bambara groundnut selected from local lines of various regions in Indonesia using the nested design. The results showed that the drought stress treatment led to stunted growth of 12 bambara groundnut genotypes, including the number of leaves, plant height, canopy diameter, leaf thickness, number of flowers, number of stems or branches, number of internodes, length of leaf stems, root length, root wet weight, canopy wet weight, canopy dry weight, root dry weight, and leaf chlorophyll content. However, drought leads to a slight increase in the width and length of the stomata opening and leaf proline contents. The cluster analysis based on stress index and sensitivity index can classify 3 expected lines originating from Gresik Regency (G1, G2, G3), falling into the drought stress-tolerant category. Accumulated proline contents cannot be used as a descriptor of tolerance to drought stresses in bambara groundnut since the expected lines with an increase in proline contents in leaves during drought stresses based on the cluster analysis do not fall into the genotypic cluster tolerant to drought stresses.


2019 ◽  
pp. 1-12 ◽  
Author(s):  
Justina Y. Talabi ◽  
Babawande A. Origbemisoye ◽  
Beatrice O. Ifesan ◽  
Victor N. Enujuigha

The nutrient composition and the acceptability of biscuit from composite flours of wheat, Bambara groundnut (Vigna subterranea), Ground bean seed (Macrotyloma) and Moringa seed (Moringa oleifera) were evaluated. Bambara groundnut (Vigna subterranea), Ground bean seed (Macrotyloma) and Moringa seed (Moringa oleifera) were dried, and processed into flour. The flour blends developed was used as a substitute for wheat flour as composite flour. The resulting mixtures were then used to produce biscuits at different ratios of wheat flour to flour blends; 100:0, 90:10, 80:20 and 70:30 level of the flour blends. The pasting properties, proximate composition, minerals, physical (spread ratio, weight, thickness and colour) and sensory properties of the composite biscuit were evaluated. The pasting properties of the flours showed that pasting temperature ranged from 68.50°C - 70.0°C and the peak viscousity range from 101.17 RVU – 207.17 RVU, while Break down (43.0 RVU) was highest in 90% wheat: 10% (Bambara- groundnut-ground bean seed- moringa seed flour) (WFF1). The protein content increased from 12.50% in the control (100% wheat flour) to a range of 14.40% - 16.19% in the biscuits; crude fibre decreased from 2.83 to 2.40 - 1.84%, ash content increased from 1.26% to a range of 1.53 - 2.01%, while carbohydrate and energy value reduced from 69.20 to 65.54 - 63.36% and 384.04 Kcal/100 g to 391.34 - 391.55 Kcal/100 g respectively. As the ratio of blends level increase, the thickness, diameter and weight increased but the spread ratio decreased. In conclusion incorporation of bambara groundnut, ground bean seed and moringa seed flour blends played important role in enhancing the nutritional properties of biscuits through improving their protein content, energy value and mineral elements especially calcium and potassium.


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