Green tea extract: A natural antimicrobial with great potential for controlling native microbiota, Listeria innocua and Escherichia coli in fresh‐cut beet leaves

2017 ◽  
Vol 38 (1) ◽  
Author(s):  
María Verónica Fernández ◽  
María Victoria Agüero ◽  
Rosa Juana Jagus
2020 ◽  
Vol 8 (1) ◽  
pp. 45-51
Author(s):  
Kabita Giri ◽  
Bijay Kumar Shrestha ◽  
Jenish Shakya ◽  
Shiv Nandan Sah ◽  
Hemanta Khanal

Urinary tract infections (UTIs) caused by drug resistant (DR) Uropathogenic Escherichia coli have become a significant worldwide public health problem. Green tea (Camellia sinensis), has been reported to have antimicrobial activities against various pathogenic bacteria. The main aim of our study was to estimate the antibacterial effect of green tea extract against drug resistant Uropathogenic E. coli isolated from urine samples of patients visiting in tertiary care hospital from eastern Nepal. During the study 360 urine samples were collected from UTI suspected patients visiting a tertiary care hospital of Biratnagar. Urine samples were cultured by using semi-quantitative culture technique and bacteria was identified by standard microbiological procedure. Antibiotic susceptibility testing was done by Kirby-Bauer Disk Diffusion method according to NCCLS (2011) guidelines. The antibacterial effect of green tea extract was performed by preparing the 95% ethanol extract in Soxhlet apparatus which was dispensed in DMSO solution and sterilized by membrane filtration. Antibacterial activity of Green Tea Extract against MDR Uropathogenic Escherichia coli was performed by making different concentration of green tea. The overall prevalence of E. coli was 27.22% in study population whereas the prevalence of MDR E. coli was 21.08%. All the isolated E. coli exhibited 100% sensitivity towards Nitrofurantoin and it was still a drug of choice for the treatment of Urinary tract infection caused by E. coli. The green tea extracts exhibited effective antibacterial activity against MDR E. coli. The MIC of Green Tea Extract was found to be 600µg/ml for 24 MDR isolates and 1000µg/ml for remaining 11 isolates. Based on the present study it is concluded that Green Tea extracts have great potential as an antimicrobial agent against E. coli. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 45-51


Food Control ◽  
2012 ◽  
Vol 25 (1) ◽  
pp. 225-232 ◽  
Author(s):  
Motokazu Nakayama ◽  
Naofumi Shigemune ◽  
Takashi Tsugukuni ◽  
Hitomi Jun ◽  
Tomoyo Matsushita ◽  
...  

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Collins Amankwaah ◽  
Jianrong Li ◽  
Jaesung Lee ◽  
Melvin A. Pascall

Edible films can be designed to serve as carriers of antimicrobial agents and be used to control pathogenic foodborne viruses and bacteria. This research tested this concept by dissolving green tea extract (GTE) in chitosan film-forming solutions (FFS) and using it to prepare dried chitosan edible films. As a control, the GTE was also dissolved in deionized water (DW). The FFS and the dried chitosan films with the GTE and the DW without chitosan were all evaluated against murine norovirus (MNV-1), Escherichia coli K12, and Listeria innocua. Both the FFS and the DW with GTE were incubated with ~107 PFU/ml of the virus suspensions for 3 h. The chitosan films with GTE were incubated for 4 and 24 h at 23±1°C. The results showed that the DW containing 1, 1.5, and 2.5% aqueous GTE, significantly (p<0.05) reduced MNV-1 plaques by 1.7, 2.5, and 3.3 logs after 3 h exposure, respectively. Similarly, FFS containing 2.5 and 5.0% GTE reduced MNV-1 counts by 2.5 and 4.0 logs, respectively, after 3 h exposure. The dried chitosan films with 5, 10, and 15% GTE were also effective against MNV-1 infectivity. After 24 h incubation, the 5 and 10% chitosan GTE films produced significant (p<0.05) titer reductions of 1.6 and 4.5 logs, respectively. Chitosan films containing 15% GTE reduced MNV-1 plaques to undetectable levels in 24 h. All chitosan GTE films reduced E. coli K12 and L. innocua populations to undetectable levels in tryptic soy broth after 24 h exposure. The results of this study showed that edible films enriched with GTE have potential to reduce both foodborne viruses and bacteria.


2016 ◽  
Vol 91 (3) ◽  
pp. 279-284 ◽  
Author(s):  
Liang Yu Wu ◽  
Hui Xiao ◽  
Wen Jing Zhao ◽  
Ping Sun ◽  
Jin Ke Lin

2001 ◽  
Vol 6 (1) ◽  
pp. 57-61 ◽  
Author(s):  
YUKIKO HARA-KUDO ◽  
TSUTOMU OKUBO ◽  
SHIN TANAKA ◽  
DJONG-CHI CHU ◽  
LEKH RAJ JUNEJA ◽  
...  

2001 ◽  
Vol 42 (4) ◽  
pp. 248-251 ◽  
Author(s):  
Emiko Isogai ◽  
Hiroshi Isogai ◽  
Kimiharu Hirose ◽  
Shunji Hayashi ◽  
Keiji Oguma

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