Valorization of American lobster ( Homarus americanus ) cooking waters: preparation and characterization of a food ingredient

2021 ◽  
Author(s):  
Ariane Tremblay ◽  
Ronan Corcuff ◽  
Charles Goulet ◽  
Samuel B. Godefroy ◽  
Alain Doyen ◽  
...  
Keyword(s):  
Homarus Americanus ◽  
American Lobster ◽  
Food Ingredient

scholarly journals Purification and characterization of a digestive cysteine proteinase from the American lobster (Homarus americanus)

1989 ◽  
Vol 263 (2) ◽  
pp. 439-444 ◽  
Author(s):  
M V Laycock ◽  
T Hirama ◽  
S Hasnain ◽  
D Watson ◽  
A C Storer
Keyword(s):  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Gel Filtration ◽  
Homarus Americanus ◽  
Exchange Chromatography ◽  
American Lobster ◽  
Digestive Juice ◽  
Effects Of Ph ◽  
Km Value

A new cysteine proteinase was isolated from the digestive juice of the American lobster (Homarus americanus). The enzyme was purified by a combination of affinity and ion-exchange chromatography and gel filtration. The cysteine proteinase accounted for 80% of the proteolytic activity in the lumen of the hepatopancreas. The most potent heavy-metal inhibitors were Hg, Cu, and Ag ions. Inhibition by organic proteinase inhibitors, including E-64 [L-trans-epoxysuccinyl-leucylamido-(4-guanidino)butane] and activation of the enzyme by 2-mercaptoethanol and dithiothreitol are characteristic of cysteine proteinases. Several similarities to papain are noted and include the N-terminal sequence, of which 22 of the first 28 amino acids are identical. Some notable differences are the higher Mr of 28,000 compared with 23,350 for papain, and the low isoelectric point (pI 4.5) of the lobster enzyme. The effects of pH and temperature on catalytic activity of the lobster proteinase were studied with benzyloxycarbonylalanine p-nitrophenyl ester as the substrate. The kcat./Km value was effectively temperature-independent between 10 and 60 degrees C. The pH-activity profile for the lobster enzyme revealed four apparent protonation states, of which only two are active.

Identification and characterization of a cDNA encoding a crustin-like, putative antibacterial protein from the American lobster Homarus americanus

2007 ◽  
Vol 44 (13) ◽  
pp. 3333-3337 ◽  
Author(s):  
Andrew E. Christie ◽  
Szymon Rus ◽  
Christopher C. Goiney ◽  
Christine M. Smith ◽  
David W. Towle ◽  
...  
Keyword(s):  
Homarus Americanus ◽  
American Lobster ◽  
Antibacterial Protein ◽  
Identification And Characterization

Characterization of SSRs from the American lobster,Homarus americanus

2006 ◽  
Vol 7 (2) ◽  
pp. 330-332 ◽  
Author(s):  
ANDREA HODGINS-DAVIS ◽  
STEVEN ROBERTS ◽  
DIANE F. COWAN ◽  
JELLE ATEMA ◽  
MICHAEL BENNIE ◽  
...  
Keyword(s):  
Homarus Americanus ◽  
American Lobster

Characterization of Exoskeletal Proteins from the American Lobster, Homarus americanus

1998 ◽  
Vol 119 (1) ◽  
pp. 189-199 ◽  
Author(s):  
Marjaana Nousiainen ◽  
Kate Rafn ◽  
Lene Skou ◽  
Peter Roepstorff ◽  
Svend Olav Andersen
Keyword(s):  
Homarus Americanus ◽  
American Lobster

Characterization of an Acyl‐CoA:retinol Acyltransferase (ARAT) Activity in the Hepatopancreas of the American Lobster ( Homarus americanus)

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Stephanie E. Dew ◽  
Collin K. Wells ◽  
Olivia M. Honaker ◽  
Yuzhang Li
Keyword(s):  
Homarus Americanus ◽  
American Lobster

Purification and characterization of a mandibular organ protein from the American lobster, Homarus americanus: a putative farnesoic acid O-methyltransferase

2004 ◽  
Vol 34 (8) ◽  
pp. 785-798 ◽  
Author(s):  
Kenneth C. Holford ◽  
Kevin A. Edwards ◽  
William G. Bendena ◽  
Stephen S. Tobe ◽  
Zhiwei Wang ◽  
...  
Keyword(s):  
Homarus Americanus ◽  
American Lobster ◽  
Purification And Characterization ◽  
Farnesoic Acid
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