Spectroscopic (LC‐ESI‐MS/MS, FT‐IR, NMR) and functional characterization of fruit seed oils extracted with green technology: A comparative study with Prunus cerasus and Punica granatum oils

2021 ◽  
Author(s):  
Bülent Başyiğit ◽  
Hidayet Sağlam ◽  
İbrahim Hayoğlu ◽  
Mehmet Karaaslan
Keyword(s):  
Comparative Study ◽  
Functional Characterization ◽  
Punica Granatum ◽  
Green Technology ◽  
Prunus Cerasus ◽  
Seed Oils ◽  
Esi Ms ◽  
Ft Ir

scholarly journals Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages

mBio ◽  
2018 ◽  
Vol 9 (5) ◽  
Author(s):  
Travis J. Wiles ◽  
Elena S. Wall ◽  
Brandon H. Schlomann ◽  
Edouard A. Hay ◽  
Raghuveer Parthasarathy ◽  
...  
Keyword(s):  
Comparative Study ◽  
Molecular Mechanisms ◽  
Genetic Manipulation ◽  
Functional Characterization ◽  
Symbiotic Bacteria ◽  
Bacterial Isolates ◽  
Major Barrier ◽  
Allelic Exchange ◽  
Genetic Techniques

ABSTRACTCorrelating the presence of bacteria and the genes they carry with aspects of plant and animal biology is rapidly outpacing the functional characterization of naturally occurring symbioses. A major barrier to mechanistic studies is the lack of tools for the efficient genetic manipulation of wild and diverse bacterial isolates. To address the need for improved molecular tools, we used a collection of proteobacterial isolates native to the zebrafish intestinal microbiota as a testbed to construct a series of modernized vectors that expedite genetic knock-in and knockout procedures across lineages. The innovations that we introduce enhance the flexibility of conventional genetic techniques, making it easier to manipulate many different bacterial isolates with a single set of tools. We developed alternative strategies for domestication-free conjugation, designed plasmids with customizable features, and streamlined allelic exchange using visual markers of homologous recombination. We demonstrate the potential of these tools through a comparative study of bacterial behavior within the zebrafish intestine. Live imaging of fluorescently tagged isolates revealed a spectrum of distinct population structures that differ in their biogeography and dominant growth mode (i.e., planktonic versus aggregated). Most striking, we observed divergent genotype-phenotype relationships: several isolates that are predicted by genomic analysis andin vitroassays to be capable of flagellar motility do not display this trait within living hosts. Together, the tools generated in this work provide a new resource for the functional characterization of wild and diverse bacterial lineages that will help speed the research pipeline from sequencing-based correlations to mechanistic underpinnings.IMPORTANCEA great challenge in microbiota research is the immense diversity of symbiotic bacteria with the capacity to impact the lives of plants and animals. Moving beyond correlative DNA sequencing-based studies to define the cellular and molecular mechanisms by which symbiotic bacteria influence the biology of their hosts is stalling because genetic manipulation of new and uncharacterized bacterial isolates remains slow and difficult with current genetic tools. Moreover, developing tools de novo is an arduous and time-consuming task and thus represents a significant barrier to progress. To address this problem, we developed a suite of engineering vectors that streamline conventional genetic techniques by improving postconjugation counterselection, modularity, and allelic exchange. Our modernized tools and step-by-step protocols will empower researchers to investigate the inner workings of both established and newly emerging models of bacterial symbiosis.

scholarly journals Functional characterization of α1 -adrenoceptor subtypes in vascular tissues using different experimental approaches:a comparative study

2003 ◽  
Vol 138 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Regina Gisbert ◽  
Yolanda Madrero ◽  
Valentina Sabino ◽  
M Antonia Noguera ◽  
M Dolores Ivorra ◽  
...  
Keyword(s):  
Comparative Study ◽  
Functional Characterization ◽  
Vascular Tissues

scholarly journals Comparative study of T84 and T84SF human colon carcinoma cells: in vitro and in vivo ultrastructural and functional characterization of cell culture and metastasis

2006 ◽  
Vol 449 (1) ◽  
pp. 48-61
Author(s):  
Riccardo Alessandro ◽  
Maria Antonietta Di Bella ◽  
Anna Maria Flugy ◽  
Simona Fontana ◽  
Francesca Damiani ◽  
...  
Keyword(s):  
Cell Culture ◽  
Comparative Study ◽  
Functional Characterization ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

Synthesis and Characterization of an Aminated D-Glucose and its Stability of Cu2+Complex

2011 ◽  
Vol 197-198 ◽  
pp. 65-68 ◽  
Author(s):  
Qiang Qiang Liao ◽  
Yi Jiu Li ◽  
Bo Xiang
Keyword(s):  
Uv Spectra ◽  
Ultraviolet Region ◽  
Buffer Solution ◽  
Nmr Spectrum ◽  
Esi Ms ◽  
H Nmr ◽  
Ft Ir ◽  
The Stability ◽  
Complex Ratio

An aminated D-glucose [N,N'-di-b-D-glucopyranosyl ethylenediamine] was prepared and thoroughly characterized by FT-IR, ESI-MS, NMR spectra and elemental analysis. Compared with D-glucose, the FT-IR spectrum of the aminated glucose showed a moderate peak at 1629~1608 cm-1which was attributed to dNH vibration, suggesting glucose reacted to ethylenediamine (en). The ESI-MS spectrum exhibited a strong peak atm/z383.2, which was assigned to the species [C14H27O10N2]-. The1H-NMR spectrum of the aminated D-glucose demonstrated the signal of the C1proton and the amino proton at 4.82~4.79ppm, illustrating the amino of ethylenediamine was substituted for the hydroxy group of C1. As for UV spectra, the aminated glucose hadn’t absorbance in the ultraviolet region while its complex with Cu2+had obvious absorption peak at about 236nm. The complex ratio of the aminated glucose to Cu2+was close to 1:1 and the stability constant of its Cu2+complex was 6.8*107in 0.01mol×L-1borax buffer solution.

Structural and functional characterization of kidney bean and field pea protein isolates: A comparative study

2015 ◽  
Vol 43 ◽  
pp. 679-689 ◽  
Author(s):  
Khetan Shevkani ◽  
Narpinder Singh ◽  
Amritpal Kaur ◽  
Jai Chand Rana
Keyword(s):  
Comparative Study ◽  
Functional Characterization ◽  
Field Pea ◽  
Kidney Bean ◽  
Protein Isolates ◽  
Pea Protein

scholarly journals Synthesis and Characterization of Bone Binding Antibiotic-1 (BBA-1), a Novel Antimicrobial for Orthopedic Applications

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1541
Author(s):  
Sumedh Kamble ◽  
Peter Valtchev ◽  
Aiken Dao ◽  
Théophile Pelras ◽  
Michael J Rogers ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Systemic Delivery ◽  
Protein Prenylation ◽  
Design And Synthesis ◽  
Ft Ir ◽  
Orthopedic Applications ◽  
Alkaline Phosphatase Expression ◽  
Rapid Binding

Osteomyelitis and orthopedic infections are major clinical problems, limited by a lack of antibiotics specialized for such applications. In this paper, we describe the design and synthesis of a novel bone-binding antibiotic (BBA-1) and its subsequent structural and functional characterization. The synthesis of BBA-1 was the result of a two-step chemical conjugation of cationic selective antimicrobial-90 (CSA-90) and the bisphosphonate alendronate (ALN) via a heterobifunctional linker. This was analytically confirmed by HPLC, FT-IR, MS and NMR spectroscopy. BBA-1 showed rapid binding and high affinity to bone mineral in an in vitro hydroxyapatite binding assay. Kirby—Baur assays confirmed that BBA-1 shows a potent antibacterial activity against Staphylococcus aureus and methicillin-resistant S. aureus comparable to CSA-90. Differentiation of cultured osteoblasts in media supplemented with BBA-1 led to increased alkaline phosphatase expression, which is consistent with the pro-osteogenic activity of CSA-90. Bisphosphonates, such as ALN, are inhibitors of protein prenylation, however, the amine conjugation of ALN to CSA-90 disrupted this activity in an in vitro protein prenylation assay. Overall, these findings support the antimicrobial, bone-binding, and pro-osteogenic activities of BBA-1. The compound and related agents have the potential to ensure lasting activity against osteomyelitis after systemic delivery.

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