Effective Rebaudioside A Separation from Stevia Extracts by Enzymatic Bioconversion

2021 ◽  
Author(s):  
Ha‐Nul Lee ◽  
Seong‐Jin Hong ◽  
HyeJin Kang ◽  
Jung‐Hyun Ju ◽  
Bo‐Ram Park ◽  
...  
Keyword(s):  
Rebaudioside A ◽  
Enzymatic Bioconversion

scholarly journals Investigations into Enzymatic Bioconversion to Form Rebaudioside A from Stevioside

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Experimental Design ◽  
Statistical Design ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Statistical Experimental Design ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Perennial Shrub ◽  
Enzymatic Bioconversion ◽  
Full Factorial

Stevia rebaudiana Bertoni is a perennial shrub from South America that produces steviol glycosides which are 200-300 times sweeter than sugar. Stevioside and rebaudioside A are the main sweetening components of its leaves. Steviol glycosides are diterpenoids whose biosynthetic pathways have four steps in common with gibberellic acid formation. The most important enzyme in the biosynthetic pathway expressed by the gene UGT76G1 is referred to as UDP-glycosyltransferase 76G1. It converts stevioside into rebaudioside A. The former has a bitter aftertaste and is a poorer sweetener but is most abundant. This enzyme can be produced in a next generation recombinant way by Escherichia coli and Saccharomyces cerevisiae. Trichoderma longibrachiatum produces the enzyme β-1,3-glucanase enzyme, which can perform a transglycosylation between stevioside to gain rebaudioside A. In our study, a full-factorial statistical experimental design that applies different glycosyl donors, temperatures, enzyme-to-substrate ratios and pH's as factors in order to achieve higher Reb A ratios in S. rebaudiana extracts after transglycosylation is reported. The presented statistical design was appropriate to indicate relevant and significant factors, providing a good basis for an upcoming experimental design of a real-world optimization.

scholarly journals Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4090
Author(s):  
Morteza Sheikhalipour ◽  
Behrooz Esmaielpour ◽  
Gholamreza Gohari ◽  
Maryam Haghighi ◽  
Hessam Jafari ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Salt Stress ◽  
Titanium Dioxide Nanoparticles ◽  
Chitosan Nanoparticles ◽  
Stevia Rebaudiana ◽  
High Salt ◽  
Steviol Glycosides ◽  
Rebaudioside A ◽  
Tio2 Nps ◽  
Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

scholarly journals The Application of Pureed Butter Beans and a Combination of Inulin and Rebaudioside A for the Replacement of Fat and Sucrose in Sponge Cake: Sensory and Physicochemical Analysis

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 254
Author(s):  
Aislinn M. Richardson ◽  
Andrey A. Tyuftin ◽  
Kieran N. Kilcawley ◽  
Eimear Gallagher ◽  
Maurice G. O’Sullivan ◽  
...  
Keyword(s):  
Physicochemical Analysis ◽  
Acceptance Testing ◽  
Rebaudioside A ◽  
Simultaneous Reduction ◽  
Sponge Cake ◽  
Overall Acceptability ◽  
Sensory Acceptance ◽  
Intensity Parameters ◽  
Sensory Parameters ◽  
Instrumental Texture

Determining minimum levels of fat and sucrose needed for the sensory acceptance of sponge cake while increasing the nutritional quality was the main objective of this study. Sponge cakes with 0, 25, 50 and 75% sucrose replacement (SR) using a combination of inulin and Rebaudioside A (Reb A) were prepared. Sensory acceptance testing (SAT) was carried out on samples. Following experimental results, four more samples were prepared where fat was replaced sequentially (0, 25, 50 and 75%) in sucrose-replaced sponge cakes using pureed butter beans (Pbb) as a replacer. Fat-replaced samples were investigated using sensory (hedonic and intensity) and physicochemical analysis. Texture liking and overall acceptability (OA) were the only hedonic sensory parameters significantly affected after a 50% SR in sponge cake (p < 0.05). A 25% SR had no significant impact on any hedonic sensory properties and samples were just as accepted as the control sucrose sample. A 30% SR was chosen for further experiments. After a 50% fat replacement (FR), no significant differences were found between 30% sucrose-replaced sponge cake samples in relation to all sensory (hedonic and intensity) parameters investigated. Flavour and aroma intensity attributes such as buttery and sweet and, subsequently, liking and OA of samples were negatively affected after a 75% FR (p < 0.05). Instrumental texture properties (hardness and chewiness (N)) did not discriminate between samples with increasing levels of FR using Pbb. Moisture content increased significantly with FR (p < 0.05). A simultaneous reduction in fat (42%) and sucrose was achieved (28%) in sponge cake samples without negatively affecting OA. Optimised samples contained significantly more dietary fibre (p < 0.05).

Investigations on the Stability of Stevioside and Rebaudioside A in Soft Drinks

2010 ◽  
Vol 58 (23) ◽  
pp. 12216-12220 ◽  
Author(s):  
Ursula Wölwer-Rieck ◽  
Werner Tomberg ◽  
Andreas Wawrzun
Keyword(s):  
Soft Drinks ◽  
Rebaudioside A ◽  
The Stability

In vitro metabolism of rebaudioside B, D, and M under anaerobic conditions: Comparison with rebaudioside A

2014 ◽  
Vol 68 (2) ◽  
pp. 259-268 ◽  
Author(s):  
Sidd Purkayastha ◽  
George Pugh ◽  
Barry Lynch ◽  
Ashley Roberts ◽  
David Kwok ◽  
...  
Keyword(s):  
In Vitro Metabolism ◽  
Rebaudioside A ◽  
Anaerobic Conditions

scholarly journals Glycemic Effects of Rebaudioside A and Erythritol in People with Glucose Intolerance

2016 ◽  
Vol 40 (4) ◽  
pp. 283 ◽  
Author(s):  
Dong Hee Shin ◽  
Ji Hye Lee ◽  
Myung Shin Kang ◽  
Tae Hoon Kim ◽  
Su Jin Jeong ◽  
...  
Keyword(s):  
Glucose Intolerance ◽  
Rebaudioside A

The effect of iodosteviol glycoside on hormonal status and the level of proinflammatory cytokines in experimental hypothyroidism

2021 ◽  
Vol 22 (1) ◽  
pp. 14-19
Author(s):  
L. F. Almakaeva ◽  
◽  
G. A. Bajburina ◽  
F. H. Kamilov ◽  
D. Yu. Grebnev ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Inflammatory Cytokines ◽  
Recovery Period ◽  
Experimental Comparison ◽  
Intragastric Administration ◽  
Hormonal Status ◽  
Rebaudioside A ◽  
Parathyroid Hormones ◽  
White Rats ◽  
Pro Inflammatory Cytokines

Aim. Study of the hormonal status and the level of pro-inflammatory cytokines in the blood plasma in experimental hypothyroidism and the effect of the organoiodine complex with rebaudioside A. Materials and methods. Endemic thyroid dysfunction was modeled in sexually mature male white rats by daily intragastric administration of thiamazole for 21 days at a dose of 25 mg / kg. In the blood serum, the content of thyrotropin, total and free thyroxine, total triiodothyronine, testosterone, luteinizing, follicle-stimulating and parathyroid hormones, corticosterone, interleukins-1-beta and -6, tumor necrosis factor-alpha was studied. The animals were divided into four groups of 10 each: control, experimental, comparison and main. Results. The development of hypothyroidism was characterized by an increase in the content of thyroid-stimulating, luteinizing, follicle-stimulating and parathyroid hormones, as well as pro-inflammatory cytokines. At the same time, testosterone levels decreased, while corticosterone levels were within physiological fluctuations. Conclusion. The use within 30 days of the recovery period after the development of hypothyroidism of the iodosaccharide complex based on steviol glycoside rebaudioside A used in the food industry daily at a dose of 25 mg / kg of rat weight led to the normalization of the content of the studied hormones and cytokines in the blood plasma, characterizing the effectiveness of the new organoiodine product.

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