scholarly journals Extraction, quantification and biochemical characterization of cereal industry by‐product cell wall

2020 ◽  
Author(s):  
Tabussam Tufail ◽  
Farhan Saeed ◽  
Imran Pasha ◽  
Muhammad Umair Arshad ◽  
Muhammad Afzaal ◽  
...  
Keyword(s):  
Cell Wall ◽  
Biochemical Characterization ◽  
Cereal Industry

Biochemical characterization of a cortexless mutant of a variant of Bacillus cereus

1976 ◽  
Vol 22 (7) ◽  
pp. 1007-1012 ◽  
Author(s):  
Susanne M. Pearce
Keyword(s):  
Cell Wall ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Bacillus Cereus ◽  
Biochemical Characterization ◽  
Diaminopimelic Acid ◽  
Peptidoglycan Synthesis ◽  
Biochemical Lesion ◽  
Do So

Previous studies on this cortexless mutant of Bacillus cereus var. alesti indicated that the forespore membrane was the site of the biochemical lesion. This hypothesis is supported by the results presented here: fatty acid composition of sporulating cells of the mutant is altered, while in vegetative cells it is comparable to the parent; soluble precursors of peptidoglycan synthesis are accumulated in the mutant, at the time of cortex formation; homogenates of the mutant prepared at the time of cortex formation are unable to incorporate tritiated diaminopimelic acid into peptidoglycan, while homogenates of cells forming germ cell wall do so to an extent comparable to that of the parent; lipid-linked intermediates are formed by the mutant as in the parent. Apparently the mutant is unable either to transfer disaccharide penta-peptide units from the carrier lipid to the growing peptidoglycan acceptor, or to transport lipid-linked intermediates across the forespore membrane.

scholarly journals Biochemical characterization of a 27kDa 1,3-β-d-glucanase from Trichoderma asperellum induced by cell wall of Rhizoctonia solani

2012 ◽  
Vol 87 (2) ◽  
pp. 1219-1223 ◽  
Author(s):  
Raquel da Silva Aires ◽  
Andrei Stecca Steindorff ◽  
Marcelo Henrique Soller Ramada ◽  
Saulo José Linhares de Siqueira ◽  
Cirano José Ulhoa
Keyword(s):  
Cell Wall ◽  
Rhizoctonia Solani ◽  
Biochemical Characterization ◽  
Trichoderma Asperellum

scholarly journals Biochemical characterization of wheat straw cell wall with special reference to bioactive profile

2018 ◽  
Vol 21 (1) ◽  
pp. 1303-1310 ◽  
Author(s):  
Tabussam Tufail ◽  
Farhan Saeed ◽  
Muhammad Imran ◽  
Muhammad Umair Arshad ◽  
Faqir Muhammad Anjum ◽  
...  
Keyword(s):  
Cell Wall ◽  
Special Reference ◽  
Wheat Straw ◽  
Biochemical Characterization

scholarly journals Genetic and Biochemical Characterization of the Cell Wall Hydrolase Activity of the Major Secreted Protein of Lactobacillus rhamnosus GG

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31588 ◽  
Author(s):  
Ingmar J. J. Claes ◽  
Geert Schoofs ◽  
Krzysztof Regulski ◽  
Pascal Courtin ◽  
Marie-Pierre Chapot-Chartier ◽  
...  
Keyword(s):  
Cell Wall ◽  
Biochemical Characterization ◽  
Lactobacillus Rhamnosus ◽  
Hydrolase Activity ◽  
Secreted Protein ◽  
Lactobacillus Rhamnosus Gg ◽  
Cell Wall Hydrolase

scholarly journals Biochemical characterization of a novel ulvan lyase from Pseudoalteromonas sp. strain PLSV

RSC Advances ◽  
2018 ◽  
Vol 8 (5) ◽  
pp. 2610-2615 ◽  
Author(s):  
Hui-Min Qin ◽  
Panpan Xu ◽  
Qianqian Guo ◽  
Xiaotao Cheng ◽  
Dengke Gao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Glucuronic Acid ◽  
Biochemical Characterization ◽  
Iduronic Acid ◽  
Green Seaweed

Ulvans, complex polysaccharides found in the ulvales (green seaweed) cell wall, contain predominantly 3-sulfated rhamnose (Rha3S) linked to either d-glucuronic acid, l-iduronic acid or d-xylose.

scholarly journals Cloning and Characterization of a Novel Invertase from the Obligate Biotroph Uromyces fabae and Analysis of Expression Patterns of Host and Pathogen Invertases in the Course of Infection

2006 ◽  
Vol 19 (6) ◽  
pp. 625-634 ◽  
Author(s):  
Ralf T. Voegele ◽  
Stefan Wirsel ◽  
Ulla Möll ◽  
Melanie Lechner ◽  
Kurt Mendgen
Keyword(s):  
Cell Wall ◽  
Expression Analysis ◽  
Biochemical Characterization ◽  
Higher Plants ◽  
Rust Fungus ◽  
Expression Patterns ◽  
Invertase Activity ◽  
Pathogen Infection ◽  
Uromyces Fabae

Invertases are key enzymes in carbon partitioning in higher plants. They gain additional importance in the distribution of carbohydrates in the event of wounding or pathogen attack. Although many researchers have found an increase in invertase activity upon infection, only a few studies were able to determine whether the source of this activity was host or parasite. This article analyzes the role of invertases involved in the biotrophic interaction of the rust fungus Uromyces fabae and its host plant, Vicia faba. We have identified a fungal gene, Uf-INV1, with homology to invertases and assessed its contribution to pathogenesis. Expression analysis indicated that transcription began upon penetration of the fungus into the leaf, with high expression levels in haustoria. Heterologous expression of Uf-INV1 in Saccharomyces cerevisiae and Pichia pastoris allowed a biochemical characterization of the enzymatic activity associated with the secreted gene product INV1p. Expression analysis of the known vacuolar and cell-wall-bound invertase isoforms of V. faba indicated a decrease in the expression of a vacuolar invertase, whereas one cell-wall-associated invertase exhibited increased expression. These changes were not confined to the infected tissue, and effects also were observed in remote plant organs, such as roots. These findings hint at systemic effects of pathogen infection. Our results support the hypothesis that pathogen infection establishes new sinks which compete with physiological sink organs.

scholarly journals Characterization of FcXTH2, a Novel Xyloglucan Endotransglycosylase/Hydrolase Enzyme of Chilean Strawberry with Hydrolase Activity

2020 ◽  
Vol 21 (9) ◽  
pp. 3380 ◽  
Author(s):  
Luis Morales-Quintana ◽  
Dina Beltrán ◽  
Ángela Mendez-Yañez ◽  
Felipe Valenzuela-Riffo ◽  
Raúl Herrera ◽  
...  
Keyword(s):  
Cell Wall ◽  
Structural Model ◽  
Biochemical Characterization ◽  
Comparative Modeling ◽  
Xyloglucan Endotransglycosylase ◽  
Protein Ligand Interactions ◽  
Modeling Methodology ◽  
Ligand Interactions ◽  
Km Value

Xyloglucan endotransglycosylase/hydrolases (XTHs) are cell wall enzymes with hydrolase (XEH) and/or endotransglycosylase (XET) activities. As they are involved in the modification of the xyloglucans, a type of hemicellulose present in the cell wall, they are believed to be very important in different processes, including growth, development, and fruit ripening. Previous studies suggest that XTHs might play a key role in development and ripening of Fragaria chiloensis fruit, and its characterization is pending. Therefore, in order to provide a biochemical characterization of the FcXTH2 enzyme to explain its possible role in strawberry development, the molecular cloning and the heterologous expression of FcXTH2 were performed. The recombinant FcXTH2 was active and displayed mainly XEH activity. The optimal pH and temperature are 5.5 and 37 °C, respectively. A KM value of 0.029 mg mL−1 was determined. Additionally, its protein structural model was built through comparative modeling methodology. The model showed a typically β-jelly-roll type folding in which the catalytic motif was oriented towards the FcXTH2 central cavity. Using molecular docking, protein-ligand interactions were explored, finding better interaction with xyloglucan than with cellulose. The data provided groundwork for understanding, at a molecular level, the enzymatic mechanism of FcXTH2, an important enzyme acting during the development of the Chilean strawberry.

Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall

2017 ◽  
Vol 486 (1) ◽  
pp. 130-136 ◽  
Author(s):  
Yohei Uehara ◽  
Shunsuke Tamura ◽  
Yusuke Maki ◽  
Kenta Yagyu ◽  
Tadashi Mizoguchi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Biochemical Characterization ◽  
Rhamnogalacturonan I
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