Ultrasound extraction of bioactive compounds from Citrus reticulata peel using electrolyzed water

2019 ◽  
Vol 43 (12) ◽  
Author(s):  
Marcela Bromberger Soquetta ◽  
Denise Tonato ◽  
Mariana Menezes Quadros ◽  
Caroline Pagnossim Boeira ◽  
Alexandre José Cichoski ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Citrus Reticulata ◽  
Electrolyzed Water ◽  
Ultrasound Extraction

scholarly journals Extraction process and method validation for bioactive compounds from Citrus reticulata cv. Chachiensis: Application of response surface methodology and HPLC–DAD

2020 ◽  
Author(s):  
Zhenying Mei ◽  
Rongfei Zhang ◽  
Zhimin Zhao ◽  
Guodong Zheng ◽  
Xinjun Xu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Liquid Chromatography ◽  
Response Surface ◽  
Bioactive Compounds ◽  
High Speed ◽  
Chinese Herb ◽  
Biological Properties ◽  
Resonance Spectroscopy ◽  
Citrus Reticulata ◽  
Chromatography Method

AbstractCitrus reticulata cv. Chachiensis, a traditional Chinese herb, has extensive medicinal and edible effects. 3′,4′,5,6,7,8-Hexamethoxyflavone (HM) and 5,6,7,8,4′-pentamethoxyflavone (PM) are main bioactive compounds in Chachiensis, which have been reported to possess various biological properties. In this study, supercritical CO2 extraction (SCE) and high-speed countercurrent chromatography (HSCCC) were utilized to prepare HM and PM from Chachiensis. The contents of target compounds were determined by a high-performance liquid chromatography method with diode-array detection (HPLC-DAD), which was validated using the following parameters: linearity, sensitivity, repeatability, stability, precision and accuracy. The SCE conditions were optimized using response surface methodology with central composite design. Obtained optimum conditions were temperature of 37.9 °C, pressure of 26.3 MPa, and modifier volume of 81.0 mL. Under above conditions, the recoveries of target compounds were 92.52 ± 0.83 and 96.36 ± 0.43%, respectively. The most appropriate solvent system for HSCCC was selected as n-hexane/ethyl acetate/methanol/water (1:0.8:1:1.2, v/v). The HSCCC fractions were detected by HPLC-DAD, liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance spectroscopy (1H NMR and 13C NMR). The results indicated that this method was successfully applied to obtain HM and PM with high purities and high recoveries from Chachiensis.

Effect of slightly acidic electrolyzed water on bioactive compounds and morphology of broccoli sprouts

2018 ◽  
Vol 105 ◽  
pp. 102-109 ◽  
Author(s):  
Lizhen Li ◽  
Jianxiong Hao ◽  
Shuhui Song ◽  
Satoru Nirasawa ◽  
Zhengqiang Jiang ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Electrolyzed Water ◽  
Broccoli Sprouts

scholarly journals Recovery of bioactive compounds from Arbutus unedo L. fruits: Comparative optimization study of maceration/microwave/ultrasound extraction techniques

2018 ◽  
Vol 109 ◽  
pp. 455-471 ◽  
Author(s):  
Bianca R. Albuquerque ◽  
M.A. Prieto ◽  
J.A. Vazquez ◽  
Maria Filomena Barreiro ◽  
Lillian Barros ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Extraction Techniques ◽  
Ultrasound Extraction ◽  
Arbutus Unedo ◽  
Optimization Study

scholarly journals Quantification of Flavonoids, Phenols and Antioxidant Potential from Dropped Citrus reticulata Blanco Fruits Influenced by Drying Techniques

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4159
Author(s):  
Dinesh Kumar ◽  
Milind S. Ladaniya ◽  
Manju Gurjar ◽  
Sunil Kumar ◽  
Sachin Mendke
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Phenolic Content ◽  
Total Phenol Content ◽  
Citrus Reticulata ◽  
Phenol Content ◽  
Citrus Reticulata Blanco ◽  
Hot Air ◽  
Freeze Dried ◽  
Drying Techniques

Physiologically dropped immature Citrus reticulata Blanco fruits are regarded as waste and discarded in the citrus orchard but are a good source of bioactive compounds including flavonoids, antioxidants and total phenols. A study was undertaken to identify and quantify these bioactive compounds and to investigate the influence of different drying techniques, namely freeze drying and hot air oven drying, on flavonoids namely flavanone glycosides, antioxidant potential and total phenol content in immature dropped fruits of Citrus reticulata Blanco. Flavonoids were quantified in high-performance liquid chromatography (HPLC). The antioxidant activity were investigated with three assays azino-bis [3-ethylbenzthiazoline-6-sulfonic acid]) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), Ferric Reducing Ability of Plasma (FRAP) and total phenol content was determined. Freeze dried samples of 12 and 14 mm size retained maximum hesperidin flavonoid content (27.03% and 27.20%) as compared to the hot air dried samples (17.99%) and retained higher phenolic content ranged from 50.54–54.19 mg GAEL−1. The antioxidant activity in freeze dried fruits was from 12.21–13.55 mM L−1 Trolox and 15.27–16.72 mM L−1 Trolox with ABTS, DPPH assay and FRAP values ranging from 7.31–9.07 mM L−1 Trolox. Significant positive correlation was found between the flavonoid hesperidin with antioxidant assays and total phenolic content (TPC). The results showed that waste citrus fruits can act as potential source of bioflavonoids, especially hesperidin, and antioxidants for pharmaceutical as well as nutraceutical industry.

Investigation of bioactive compounds in the genus Garcinia (Guttiferae) of Cameroon

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
B Biloa Messi ◽  
R Ho ◽  
A Meli Lannang ◽  
JG Tangmouo ◽  
A Marston ◽  
...  
Keyword(s):  
Bioactive Compounds

Strategies and Methods for a Sustainable Search for Bioactive Compounds

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
L Bohlin ◽  
C Alsmark ◽  
U Göransson ◽  
M Klum ◽  
C Wedén ◽  
...  
Keyword(s):  
Bioactive Compounds
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