Lutein‐fortified potato soup and freeze‐dried lutein powder designed with supercritical carbon dioxide extract of yellow corn kernels are promising nutraceutical foods

2019 ◽  
Vol 43 (8) ◽  
Author(s):  
Sayani Pal ◽  
Paramita Bhattacharjee
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Yellow Corn ◽  
Freeze Dried ◽  
Corn Kernels ◽  
Supercritical Carbon

scholarly journals Compound Identification and In Vitro Cytotoxicity of the Supercritical Carbon Dioxide Extract of Papaya Freeze-Dried Leaf Juice

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 610
Author(s):  
Kooi-Yeong Khaw ◽  
Paul Nicholas Shaw ◽  
Marie-Odile Parat ◽  
Saurabh Pandey ◽  
James Robert Falconer
Keyword(s):  
Mass Spectrometry ◽  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Activities ◽  
Compound Identification ◽  
Freeze Dried ◽  
Leaf Juice ◽  
Supercritical Carbon

Carica papaya leaves are used as a remedy for the management of cancer. Freeze-dried C. papaya leaf juice was extracted using a supercritical fluid extraction system. Compound identification was carried out using analytical techniques including liquid chromatography coupled to high-resolution quadrupole time-of-flight mass spectrometry (LC–QToF-MS) and gas chromatography–mass spectrometry (GC–MS). The cytotoxic activities of the scCO2 extract and its chemical constituents were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on squamous cell carcinoma (SCC25) and human keratinocyte (HaCaT) cell lines. The chemical constituents were quantified by QToF-MS. The supercritical carbon dioxide (scCO2) extract of papaya freeze-dried leaf juice showed cytotoxic activity against SCC25. Three phytosterols, namely, β-sitosterol, campesterol, and stigmasterol, together with α-tocopherol, were confirmed to be present in the scCO2 extract. Quantitative analysis showed that β-sitosterol was the major phytosterol present followed by α-tocopherol, campesterol, and stigmasterol. β-Sitosterol and campesterol were active against SCC25 (half maximal inhibitory concentration (IC50) ≈ 1 µM), while stigmasterol was less active (~33 µM) but was biologically more selective against SCC25. Interestingly, an equimolar mixture of phytosterols was not more effective (no synergistic effect was observed) but was more selective than the individual compounds. The compounds identified are likely accountable for at least part of the cytotoxicity and selectivity effects of C. papaya.

Effect of Supercritical Carbon Dioxide on Physicochemical Properties of Freeze-Dried Bovine Liver

2015 ◽  
Vol 49 (4) ◽  
pp. 245-254
Author(s):  
Hye-Min Kim ◽  
◽  
Sung-Woon Woo ◽  
Sung-Won Kang ◽  
Ho-Jin Heo ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Physicochemical Properties ◽  
Bovine Liver ◽  
Freeze Dried ◽  
Supercritical Carbon
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