Antibacterial Activity of H elichrysum italicum Oil on Vegetables and Its Mechanism of Action

2015 ◽  
Vol 39 (6) ◽  
pp. 2663-2672 ◽  
Author(s):  
Haiying Cui ◽  
Chengting Zhao ◽  
Lin Lin
Keyword(s):  
Antibacterial Activity ◽  
Mechanism Of Action

scholarly journals Proteomic response of Escherichia coli to a membrane lytic and iron chelating truncated Amaranthus tricolor defensin

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Tessa B. Moyer ◽  
Ashleigh L. Purvis ◽  
Andrew J. Wommack ◽  
Leslie M. Hicks
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antimicrobial Peptides ◽  
Mechanism Of Action ◽  
Gram Negative Bacteria ◽  
Amaranthus Tricolor ◽  
Core Motif ◽  
Gram Negative ◽  
E Coli ◽  
Membrane Lysis

Abstract Background Plant defensins are a broadly distributed family of antimicrobial peptides which have been primarily studied for agriculturally relevant antifungal activity. Recent studies have probed defensins against Gram-negative bacteria revealing evidence for multiple mechanisms of action including membrane lysis and ribosomal inhibition. Herein, a truncated synthetic analog containing the γ-core motif of Amaranthus tricolor DEF2 (Atr-DEF2) reveals Gram-negative antibacterial activity and its mechanism of action is probed via proteomics, outer membrane permeability studies, and iron reduction/chelation assays. Results Atr-DEF2(G39-C54) demonstrated activity against two Gram-negative human bacterial pathogens, Escherichia coli and Klebsiella pneumoniae. Quantitative proteomics revealed changes in the E. coli proteome in response to treatment of sub-lethal concentrations of the truncated defensin, including bacterial outer membrane (OM) and iron acquisition/processing related proteins. Modification of OM charge is a common response of Gram-negative bacteria to membrane lytic antimicrobial peptides (AMPs) to reduce electrostatic interactions, and this mechanism of action was confirmed for Atr-DEF2(G39-C54) via an N-phenylnaphthalen-1-amine uptake assay. Additionally, in vitro assays confirmed the capacity of Atr-DEF2(G39-C54) to reduce Fe3+ and chelate Fe2+ at cell culture relevant concentrations, thus limiting the availability of essential enzymatic cofactors. Conclusions This study highlights the utility of plant defensin γ-core motif synthetic analogs for characterization of novel defensin activity. Proteomic changes in E. coli after treatment with Atr-DEF2(G39-C54) supported the hypothesis that membrane lysis is an important component of γ-core motif mediated antibacterial activity but also emphasized that other properties, such as metal sequestration, may contribute to a multifaceted mechanism of action.

Kaurenoic acid and its sodium salt derivative: antibacterial activity againstPorphyromonas gingivalisand their mechanism of action

2018 ◽  
Vol 13 (14) ◽  
pp. 1585-1601 ◽  
Author(s):  
Carlos HG Martins ◽  
Fariza Abrão ◽  
Thaís S Moraes ◽  
Pollyanna F Oliveira ◽  
Denise C Tavares ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Mechanism Of Action ◽  
Sodium Salt ◽  
Kaurenoic Acid

scholarly journals Antibacterial activity and mechanism of action of analogues derived from the antimicrobial peptide mBjAMP1 isolated from Branchiostoma japonicum

2018 ◽  
Vol 73 (8) ◽  
pp. 2054-2063 ◽  
Author(s):  
Jonggwan Park ◽  
Hee Kyoung Kang ◽  
Moon-Chang Choi ◽  
Jeong Don Chae ◽  
Byoung Kwan Son ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antimicrobial Peptide ◽  
Mechanism Of Action

Essential oil from pink pepper (Schinus terebinthifolius Raddi): Chemical composition, antibacterial activity and mechanism of action

Food Control ◽  
2019 ◽  
Vol 95 ◽  
pp. 115-120 ◽  
Author(s):  
Guilherme da Silva Dannenberg ◽  
Graciele Daiana Funck ◽  
Wladimir Padilha da Silva ◽  
Ângela Maria Fiorentini
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Mechanism Of Action ◽  
Schinus Terebinthifolius

scholarly journals Antibacterial Activity and Mechanism of Action of Black Pepper Essential Oil on Meat-Borne Escherichia coli

2017 ◽  
Vol 7 ◽  
Author(s):  
Jing Zhang ◽  
Ke-Ping Ye ◽  
Xin Zhang ◽  
Dao-Dong Pan ◽  
Yang-Ying Sun ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Mechanism Of Action ◽  
Black Pepper

Effects of Copaifera duckei Dwyer oleoresin on the cell wall and cell division of Bacillus cereus

2013 ◽  
Vol 62 (7) ◽  
pp. 1032-1037 ◽  
Author(s):  
Elizabeth Cristina Gomes dos Santos ◽  
Claudio Luis Donnici ◽  
Elizabeth Ribeiro da Silva Camargos ◽  
Adriana Augusto de Rezende ◽  
Eloisa Helena de Aguiar Andrade ◽  
...  
Keyword(s):  
Cell Wall ◽  
Antibacterial Activity ◽  
Cell Division ◽  
Bacillus Cereus ◽  
Mechanism Of Action ◽  
Gram Negative Bacteria ◽  
Bactericidal Action ◽  
Transmission Electron ◽  
Sds Page ◽  
Division Process

The aim of this work was to evaluate the antibacterial activity of Copaifera duckei oleoresin and to determine its possible mechanism of action against bacteria of clinical and food interest. The antibacterial activity was determined by agar diffusion and dilution methods; the mechanism of action by transmission electron microscopy and by SDS-PAGE; the bioactive compounds by bioautography; and the chemical analysis by GC/MS. Oleoresin showed activity against nine of the 11 strains of bacteria tested. Bacillus cereus was the most sensitive, with a MIC corresponding to 0.03125 mg ml−1 and with a bactericidal action. Oleoresin acted on the bacterial cell wall, removing proteins and the S-layer, and interfering with the cell-division process. This activity probably can be attributed to the action of terpenic compounds, among them the bisabolene compound. Gram-negative bacteria tested were not inhibited. C. duckei oleoresin is a potential antibacterial, suggesting that this oil could be used as a therapeutic alternative, mainly against B. cereus.

Antimicrobial Capacity of Plant Polyphenols against Gram-positive Bacteria: A Comprehensive Review

2020 ◽  
Vol 27 (15) ◽  
pp. 2576-2606 ◽  
Author(s):  
Francisco Javier Álvarez-Martínez ◽  
Enrique Barrajón-Catalán ◽  
José Antonio Encinar ◽  
Juan Carlos Rodríguez-Díaz ◽  
Vicente Micol
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Mechanism Of Action ◽  
Molecular Targets ◽  
Membrane Receptors ◽  
Resistant Bacteria ◽  
Plant Polyphenols ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Promising Source

Background: Multi-drug-resistant bacteria such as Methicillin-Resistant Staphylococcus aureus (MRSA) disseminate rapidly amongst patients in healthcare facilities and suppose an increasingly important cause of community-associated infections and associated mortality. The development of effective therapeutic options against resistant bacteria is a public health priority. Plant polyphenols are structurally diverse compounds that have been used for centuries for medicinal purposes, including infections treatment and possess, not only antimicrobial activity, but also antioxidant, anti-inflammatory and anticancer activities among others. Based on the existing evidence on the polyphenols’ antibacterial capacity, polyphenols may be postulated as an alternative or complementary therapy for infectious diseases. Objective: To review the antimicrobial activity of plant polyphenols against Gram-positive bacteria, especially against S. aureus and its resistant strains. Determine the main bacterial molecular targets of polyphenols and their potential mechanism of action. Methodology: The most relevant reports on plant polyphenols’ antibacterial activity and their putative molecular targets were studied. We also performed virtual screening of thousand different polyphenols against proteins involved in the peptidoglycan biosynthesis to find potential valuable bioactive compounds. The bibliographic information used in this review was obtained from MEDLINE via PubMed. Results: Several polyphenols: phenolic acids, flavonoids (especially flavonols), tannins, lignans, stilbenes and combinations of these in botanical mixtures, have exhibited significant antibacterial activity against resistant and non-resistant Gram-positive bacteria at low μg/mL range MIC values. Their mechanism of action is quite diverse, targeting cell wall, lipid membrane, membrane receptors and ion channels, bacteria metabolites and biofilm formation. Synergic effects were also demonstrated for some combinations of polyphenols and antibiotics. Conclusion: Plant polyphenols mean a promising source of antibacterial agents, either alone or in combination with existing antibiotics, for the development of new antibiotic therapies.

Sign in / Sign up

Export Citation Format

Share Document